EGFR

Gene Symbol EGFR
Entrez Gene 1956
Alt Symbol ERBB, ERBB1, HER1, NISBD2, PIG61, mENA
Species Human
Gene Type protein-coding
Description epidermal growth factor receptor
Other Description avian erythroblastic leukemia viral (v-erb-b) oncogene homolog|cell growth inhibiting protein 40|cell proliferation-inducing protein 61|erb-b2 receptor tyrosine kinase 1|proto-oncogene c-ErbB-1|receptor tyrosine-protein kinase erbB-1
Swissprots Q9H3C9 Q9H2C9 Q9UMD7 Q9UMG5 Q14225 Q9UMD8 O00732 O00688 Q68GS5 P00533 Q92795 Q9BZS2 P06268 Q9GZX1
Accessions AAA52370 AAA63171 AAC50796 AAC50797 AAC50798 AAC50799 AAC50800 AAC50801 AAC50803 AAC50804 AAG35786 AAG35787 AAG35788 AAG35789 AAG35790 AAG43243 AAG43244 AAG43245 AAS07524 AAS83109 AFK93471 AFK93472 AFK93473 AFK93474 AFK93475 AFK93476 AFK93477 AFK93478 AIE16170 AIE16171 BAE15958 BAF79636 BAF80003 BAF80004 BAL40868 BAL41217 CAA29668 CAA34902 CAJ55813 CAM91181 CAV33298 CBK51921 CBK51922 CBK51923 CBK51924 CCO13722 CCO75437 CCO75439 CCV20014 EAW50962 EAW50963 EAW50964 EAW50965 P00533 AB209442 BAD92679 AF125253 AAG43240 AF277897 AAK01080 AI217671 AK127817 AK225422 AK290352 BAF83041 AK294750 BAH11869 AL598260 AU137334 AW163038 AW295229 AY550966 AAT52212 AY698023 AAT97978 AY698024 AAT97979 BC070081 BC094761 AAH94761 BC118665 AAI18666 BC128419 AAI28420 CB160831 DQ088980 AAZ66620 EF210210 ABQ66237 EF210211 ABQ66238 GU228573 GU228584 GU255993 ADL28125 HM437234 ADN43066 HM437235 HQ730875 HQ912715 ADZ75461 HQ999997 ADY76965 K03193 AAA52371 U48722 AAC50802 U51732 U95089 AAB53063 X00588 CAA25240 X00663 CAA25282 NM_005228 NP_005219 NM_201282 NP_958439 NM_201283 NP_958440 NM_201284 NP_958441
Function Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS- RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-ca
Subcellular Location Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome. Endosome membrane. Nucleus. Note=In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF).
Tissue Specificity Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers. {ECO:0000269|PubMed:17671655}.
Top Pathways Estrogen signaling pathway, Oxytocin signaling pathway, MicroRNAs in cancer, Proteoglycans in cancer, Central carbon metabolism in cancer

PathScan ® Phospho-EGF Receptor (Tyr1068) Sandwich ELISA Kit - 7240 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1: Treatment of A431 cells with EGF stimulates phosphorylation of EGF Receptor at Tyr1068, detected by PathScan® Phospho-EGF Receptor (Tyr1068) Sandwich ELISA kit #7240, but does not affect the level of total EGF Receptor detected by PathScan® Total EGF Receptor Sandwich ELISA kit #7250. OD 450 nm readings are shown in the top figure, while the corresponding Western blot using Phospho-EGF Receptor (Tyr1068) Antibody #2234 (right panel) or EGF Receptor Antibody #2232 (left panel), is shown in the bottom figure.

PathScan ® Phospho-EGF Receptor (Tyr845) Sandwich ELISA Kit - 7189 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 2: The relationship between protein concentration of lysates from untreated and EGF-treated A431 cells and kit assay optical density readings. After starvation, A431 cells (85% confluence) were treated with EGF (100 ng/ml) for 5 min at 37°C, and then lysed.

PathScan ® Phospho-EGF Receptor (Tyr1173) Sandwich ELISA Kit - 7187 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 2: The relationship between protein concentration of lysates from untreated and EGF-treated A431 cells and kit assay optical density readings. After starvation, A431 cells (85% confluence) were treated with EGF (100 ng/ml) for 5 min at 37°C, and then lysed.

PathScan ® Phospho-EGF Receptor (Tyr1068) Chemiluminescent Sandwich ELISA Kit - 7036 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1. The relationship between protein concentration of lysates from A-431 cells, untreated or treated with hEGF #8916, and immediate light generation with chemiluminescent substrate. After starvation, A-431 cells (85% confluence) were treated with EGF (100 ng/ml, 5 min at 37°C) and then lysed. Graph inset corresponding to the shaded area shows high sensitivity and a linear response at the low protein concentration range.

PathScan ® Phospho-EGF Receptor (panTyr) Sandwich ELISA Kit - 7911 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1. Treatment of A-431 cells with hEGF #8916 stimulates tyrosine phosphorylation of EGF receptor detected by PathScan ® Phospho-EGF Receptor (panTyr) Sandwich ELISA Kit #7911, but does not affect the level of total EGF receptor detected by PathScan ® Total EGF Receptor Sandwich ELISA Kit #7250. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blots using EGF Receptor Antibody #2232 (left panel) or Phospho-EGF Receptor (Tyr1173) (53A2) Rabbit mAb #4407 (right panel) are shown in the bottom figure.

PathScan ® Total EGF Receptor Sandwich ELISA Kit - 7250 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 2: The relationship between protein concentration of lysates from untreated and EGF-treated A431 cells and kit assay optical density readings. After starvation, A431 cells (85% confluence) were treated with EGF (100 ng/ml) for 5 min at 37°C, and then lysed.

PathScan ® Total EGF Receptor Chemiluminescent Sandwich ELISA Kit - 7297 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1. The relationship between protein concentration of lysates from A-431 cells, untreated or treated with hEGF #8916, and immediate light generation with chemiluminescent substrate. After starvation, A-431 cells (85% confluence) were treated with hEGF (100 ng/ml, 5 min at 37°C) and then lysed. Graph inset corresponding to the shaded area shows high sensitivity and a linear response at the low protein concentration range.

PathScan ® EGF Receptor (E746-A750del Specific) Sandwich ELISA Kit - 8950 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)

PathScan ® EGF Receptor (L858R Mutant Specific) Sandwich ELISA Kit - 8951 from Cell Signaling Technology

Category ELISA Kit

HTScan® EGF Receptor Kinase Assay Kit - 7909 from Cell Signaling Technology

Category Assay Kit
Figure 1. EGFR kinase activity was measured in a radiometric assay using the following reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 5 mM MgCl2, 5 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, 100 µM ATP, 250 µM PTP1B (Tyr66) Biotinylated Peptide (#1325) and variable amount of Recombinant EGFR. Reaction mixture incubated at room temperature for 10 minutes.

HTScan® EGFR Kinase Assay Kit - 7410 from Cell Signaling Technology

Category Assay Kit
Figure 5. EGF-R kinase activity was measured in a radioisotopic filtration assay using the following reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 uM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 ug / 50 ul PEG20.000, Substrate:PolyEY, 10 ug/50 ul, Recombinant EGF-R: 5 Units/50 ul.

EGFR (ERBB) Human ELISA Kit - ab100505 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
Sensitivity < 4 pg/ml
Range 2.74 pg/ml - 2000 pg/ml
EGFR measured in cell lysates showing quantity (ng) per 1 million cells

EGFR (pS1070) + total EGFR Human ELISA Kit - ab126435 from Abcam

Category ELISA Kit
Prices $499.00
Sizes 1 x 96 tests
A431 cells were treated with recombinant human EGF at 37°C for 20 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.

EGFR (pS1070) Human ELISA Kit - ab126434 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
A431 cells were treated with recombinant human EGF at 37°C for 20 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.

EGFR (pY1045) + total EGFR Human ELISA Kit - ab126437 from Abcam

Category ELISA Kit
Prices $499.00
Sizes 1 x 96 tests
A431 cells were treated with recombinant Human EGF at 37°C for 20 min. Solubilize cells at 2 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.

EGFR (pY1045) Human ELISA Kit - ab126436 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
A431 cells were treated with recombinant Human EGF at 37°C for 20 min. Solubilize cells at 2 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.

EGFR (pY1068) + total EGFR Human ELISA Kit - ab126439 from Abcam

Category ELISA Kit
Prices $499.00
Sizes 1 x 96 tests
A431 cells were treated with recombinant human EGF at 37°C for 10 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed using ab126439.

EGFR (pY1068) Human ELISA Kit - ab126438 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
A431 cells were treated with recombinant human EGF at 37°C for 10 min. Cells were solubilized at 4 x 107 cells/ml in Lysis Buffer. Serial dilutions of lysates were analyzed using ab126438.

EGFR (pY1086) + total EGFR Human ELISA Kit - ab126441 from Abcam

Category ELISA Kit
Prices $499.00
Sizes 1 x 96 tests
A431 cells were treated with recombinant human EGF at 37°C for 20 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.

EGFR (pY1086) Human ELISA Kit - ab126440 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
A431 cells were treated with recombinant human EGF at 37°C for 20 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed using ab126440.

EGFR (pY992) + total EGFR Human ELISA Kit - ab126443 from Abcam

Category ELISA Kit
Prices $499.00
Sizes 1 x 96 tests
A431 cells were treated with recombinant human EGF at 37°C for 20 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.

EGFR (pY992) Human ELISA Kit - ab126442 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
A431 cells were treated with recombinant Human EGF at 37°C for 20 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed using ab126442.

EGFR (Tyr1068) Human In-Cell ELISA Kit - ab126420 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
A431 cells were stimulated by different concentrations of EGF for 10 min at 37°C.

EGFR (Tyr845) In-Cell ELISA Kit - ab126421 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
A431 cells were stimulated by different concentrations of EGF for 10 min at 37°C.
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