MGAT5

Gene Symbol MGAT5
Entrez Gene 4249
Alt Symbol GNT-V, GNT-VA
Species Human
Gene Type protein-coding
Description mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase
Other Description N-acetylglucosaminyl-transferase V|alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A|alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase|glcNAc-T V|mannoside acetylglucosaminyltransferase 5
Swissprots Q09328 D3DP70
Accessions AAB81939 AAX93248 AAY14747 AAY14791 EAX11657 EAX11658 Q09328 AF055029 AF113921 AAD22449 AK056461 AK125207 BC041917 BC156313 AAI56314 BC157048 AAI57049 BU191961 BX647087 D17716 BAA04570 DB223638 DB237089 XM_005263668 XP_005263725 XM_005263669 XP_005263726 XM_005263670 XP_005263727 XM_006712534 XP_006712597 XM_006712535 XP_006712598 XM_011511199 XP_011509501 XM_011511200 XP_011509502 XM_011511201 XP_011509503 XM_011511202 XP_011509504 XM_011511203 XP_011509505 XM_011511204 XP_011509506 NM_002410 NP_002401
Function Catalyzes the addition of N-acetylglucosamine in beta 1- 6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides.
Subcellular Location Golgi apparatus membrane; Single-pass type II membrane protein.
Top Pathways N-Glycan biosynthesis

GnT-V (S-15) - sc-19088 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GnT-V (S-15): sc-19088. Western blot analysis of GnT-V expression in non-transfected: sc-117752 (A) and human GnT-V transfected: sc-159554 (B) 293T whole cell lysates.

GnT-V (T-19) - sc-34140 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GnT-V (T-19): sc-34140. Western blot analysis of GnT-V expression in non-transfected: sc-117752 (A) and human GnT-V transfected: sc-159554 (B) 293T whole cell lysates.

GnT-V (H-126) - sc-366149 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
GnT-V (H-126): sc-366149. Western blot analysis of GnT-V expression in c4 whole cell lysate.

Anti-MGAT5 antibody - ab87977 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Overlay histogram showing Jurkat cells stained with ab87977 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab87977, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-MGAT5 antibody - ab68595 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MGAT5 antibody (ab68595) at 1 µg/mlLane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 3 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

Anti-MGAT5 antibody - ab89267 from Abcam

Prices $370.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
ab89267 at 1/100 dilution staining MGAT5 in human liver by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue showing cytoplasmic staining (left image). Right image is blocked with immunizing peptide.

Anti-GNT-V / MGAT5 Antibody (aa652-680, APC) - LS-C263127 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GNT-V / MGAT5 Antibody (aa652-680, AP) - LS-C263125 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GNT-V / MGAT5 Antibody (aa652-680, FITC) - LS-C263129 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GNT-V / MGAT5 Antibody (aa652-680, PE) - LS-C263131 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GNT-V / MGAT5 Antibody (aa652-680, Azide-free, HRP) - LS-C263130 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GNT-V / MGAT5 Antibody (aa651-681) - LS-C323012 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GNT-V / MGAT5 Antibody (aa652-680, Biotin) - LS-C263128 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GNT-V / MGAT5 Antibody (C-Terminus) - LS-C345438 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of MGAT5 Antibody with human OVCAR-3 Whole Cell lysate.

Anti-GNT-V / MGAT5 Antibody (aa652-680) - LS-C164175 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
MGAT5 Antibody western blot of NCI-H460 cell line lysates (35 ug/lane). The MGAT5 antibody detected the MGAT5 protein (arrow).

Anti-GNT-V / MGAT5 Antibody (clone 3E9) - LS-C133911 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3E9
Immunoperoxidase of monoclonal antibody to MGAT5 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]

N-Acetylglucosaminyltransferase V/MGAT5 Antibody (706824) - MAB5469 from Novus Biologicals

Prices $99.00, $249.00
Sizes 25 µg, 100 µg
Host Mouse
Clonality Monoclonal
Clone 706824
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human N‑Acetylglucosaminyltransferase V/MGAT5 Monoclonal Antibody (Catalog # MAB5469) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for N‑Acetylglucosaminyltransferase V/MGAT5 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

N-Acetylglucosaminyltransferase V/MGAT5 Antibody (3E9) - H00004249-M09 from Novus Biologicals

Prices $349.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3E9
Western Blot: MGAT5 Antibody (3E9) [H00004249-M09] - MGAT5 monoclonal antibody (M09), clone 3E9. Western Blot analysis of MGAT5 expression in Jurkat(Cat # L017V1 ).

N-Acetylglucosaminyltransferase V/MGAT5 Antibody - NBP1-83354 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry: N-Acetylglucosaminyltransferase V/MGAT5 Antibody [NBP1-83354] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.

N-Acetylglucosaminyltransferase V/MGAT5 Antibody - 28400002 from Novus Biologicals

Prices $329.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ELISA: N-Acetylglucosaminyltransferase V/MGAT5 Antibody [28400002]

N-Acetylglucosaminyltransferase V/MGAT5 Antibody - H00004249-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal

MGAT5 Polyclonal Antibody - bs-5841R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Monoclonal Anti-MGAT5 antibody produced in mouse - SAB1401244 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3E9
<B>Immunoblotting</B><BR/>Monoclonal Anti-MGAT5, antibody produced in mouse, clone 3E9, purified immunoglobulin: <B>Cat. No. SAB1401244</B>: Antibody concentration: 1.0 - 5.0 μg/mL. Specific band of ~84.54 kDa using Jurkat lysate.

Anti-MGAT5 antibody produced in rabbit - HPA010919 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-MGAT5: <B>Cat. No. HPA010919</B>: Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.

ANTI-MGAT5(C-TERMINAL) antibody produced in rabbit - SAB1306957 from Sigma-Aldrich

Prices $326.00
Sizes 40 tst
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-MGoat5 (C-term): <B>Cat. No. SAB1306957</B>: MGoat5 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35 μg/lane).
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