ZWILCH

Gene Symbol ZWILCH
Entrez Gene 55055
Alt Symbol KNTC1AP, hZwilch
Species Human
Gene Type protein-coding
Description zwilch kinetochore protein
Other Description Zwilch, kinetochore associated, homolog|homolog of Drosophila Zwilch|protein zwilch homolog
Swissprots Q96SY7 Q8N404 Q9NWG7 B3KVB8 Q9H900 Q6N049
Accessions EAW77777 EAW77778 EAW77779 Q9H900 AK000898 AK023175 BAB14446 AK027468 BAB55133 AK122787 BAG53730 AK291048 BAF83737 BC036900 AAH36900 BC090041 AAH90041 BQ775697 BX640701 CAE45821 NR_003105 NM_001287821 NP_001274750 NM_001287822 NP_001274751 NM_001287823 NP_001274752 NM_017975 NP_060445
Function Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. {ECO:0000269|PubMed:15824131}.
Subcellular Location Chromosome, centromere, kinetochore {ECO:0000269|PubMed:20462495}.

Zwilch (C-18) - sc-66302 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Zwilch (S-20) - sc-66305 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Zwilch (NB-C38) - sc-135615 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Zwilch (NB-C38): sc-135615. Western blot analysis of Zwilch expression in human Zwilch transfected (A) and non-transfected (B) 293T whole cell lysates.

Anti-ZWILCH antibody - ab154534 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-ZWILCH antibody (ab154534) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg

Anti-ZWILCH antibody - ab57533 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab57533 ZWILCH antibody at 1ug/ml.

Anti-ZWILCH antibody - ab105117 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
HeLa cell lysate immunoprecipitated using ab105117 (Left Lane) at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane) for the analysis of ZWILCH expression. Subsequent WB detection was performed using ab105117 at 1 µg/ml (as the sample is enriched by IP). Rightr Lane represents IP with IgG control. Exposure time: 10 seconds.

Anti-ZWILCH antibody - C-terminal - ab174618 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-ZWILCH antibody - C-terminal (ab174618) at 1/100 dilution + Mouse spleen lysate at 35 µg

Anti-ZWILCH Antibody (aa482-508, AP) - LS-C243598 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ZWILCH Antibody (aa482-508, APC) - LS-C243600 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ZWILCH Antibody (aa482-508, FITC) - LS-C243602 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ZWILCH Antibody (aa482-508, PE) - LS-C243603 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ZWILCH Antibody (aa482-508, Biotin) - LS-C243601 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ZWILCH Antibody (aa541-591) - LS-C289662 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ZWILCH Antibody (aa482-508, Azide-free, HRP) - LS-C247423 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ZWILCH Antibody (aa481-508) - LS-C326759 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ZWILCH Antibody (aa482-508) - LS-C157530 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
ZWILCH Antibody western blot of mouse spleen tissue lysates (35 ug/lane). The ZWILCH antibody detected the ZWILCH protein (arrow).

ZWILCH Antibody - NBP1-84651 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: ZWILCH Antibody [NBP1-84651] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10<br/>Lane 2: Human cell line RT-4<br/>Lane 3: Human cell line U-251MG sp

ZWILCH Antibody - NBP2-21057 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: ZWILCH Antibody [NBP2-21057] - Sample (30 ug of whole cell lysate) A: MCF-7 7. 5% SDS PAGE gel, diluted at 1:1000.

ZWILCH Antibody (1C9) - H00055055-M01 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1C9
Western Blot: ZWILCH Antibody (1C9) [H00055055-M01] - Analysis of ZWILCH expression in transfected 293T cell line by ZWILCH monoclonal antibody (M01), clone 1C9.Lane 1: ZWILCH transfected lysate(54.224 KDa).Lane 2: Non-transfected lysate.

ZWILCH Antibody - NBP1-49690 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunoprecipitation: ZWILCH Antibody [NBP1-49690] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells.  Affinity purified rabbit anti-Zwilch antibody used for IP at 3 mcg/mg lysate.  Zwilch was also immunoprecipitated by another rabbit anti-Zwilch antibody, which recognizes an upstream epitope.

ZWILCH Antibody - NBP2-21058 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: ZWILCH Antibody [NBP2-21058] - Sample (30 ug of whole cell lysate) A: IMR32 7. 5% SDS PAGE gel, diluted at 1:1000.

ZWILCH Antibody - H00055055-D01P from Novus Biologicals

Prices $399.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

ZWILCH Antibody - H00055055-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: ZWILCH Antibody [H00055055-B01] - analysis of ZWILCH expression in transfected 293T cell line by ZWILCH MaxPab polyclonal antibody (B01). Lane 1: ZWILCH transfected lysate (54.20 KDa). Lane 2: Non-transfected lysate.

ZWILCH Antibody (1C10) - H00055055-M11 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1C10

Monoclonal Anti-ZWILCH antibody produced in mouse - SAB1405139 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1C10
<B>Immunoblotting</B><BR/>Monoclonal Anti-ZWILCH: <B>Cat. No. SAB1405139</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~54.2 kDa using ZWILCH transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.
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