DDB1

Gene Symbol DDB1
Entrez Gene 1642
Alt Symbol DDBA, UV-DDB1, XAP1, XPCE, XPE, XPE-BF
Species Human
Gene Type protein-coding
Description damage-specific DNA binding protein 1, 127kDa
Other Description DDB p127 subunit|DNA damage-binding protein 1|DNA damage-binding protein a|HBV X-associated protein 1|UV-DDB 1|UV-damaged DNA-binding factor|UV-damaged DNA-binding protein 1|XAP-1|XPE-binding factor|xeroderma pigmentosum group E-complementing protein
Swissprots O15176 Q58F96 B2R648 Q16531 Q13289 B4DG00 A6NG77
Accessions AAX44048 EAW73933 EAW73934 EAW73935 EAW73936 EAW73937 Q16531 AA665872 AJ002955 CAA05770 AK294341 BAG57611 AK294493 BAH11787 AK299650 BAG61570 AK302924 BAH13845 AK303032 BAG64157 AK311476 AK312436 BAG35345 AL831958 BC011686 AAH11686 BC021044 BC032080 BC050530 AAH50530 BC051764 AAH51764 CD242268 DC334780 L40326 AAA62838 U18299 AAC50349 U32986 AAA88883 NM_001923 NP_001914
Function Required for DNA repair. Binds to DDB2 to form the UV- damaged DNA-binding protein complex (the UV-DDB complex). The UV- DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin- protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate t
Subcellular Location Cytoplasm. Nucleus. Note=Primarily cytoplasmic. Translocates to the nucleus following UV irradiation and subsequently accumulates at sites of DNA damage.
Top Pathways Ubiquitin mediated proteolysis, Nucleotide excision repair, Viral carcinogenesis

DDB-1 (D4C8) Rabbit mAb - 6998 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D4C8
Western blot analysis of extracts from various cell lines using DDB-1 (D4C8) Rabbit mAb.

DDB-1 Antibody - 5428 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using DDB-1 Antibody.

DDB1 (B-1) - sc-137142 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
DDB1 (B-1): sc-137142. Western blot analysis of DDB1 expression in non-transfected: sc-117752 (A) and mouse DDB1 transfected: sc-119698 (B) 293T whole cell lysates.

DDB1 (V-17) - sc-16292 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
DDB1 (V-17): sc-16292. Western blot analysis of DDB1 expression in non-transfected: sc-117752 (A) and human DDB1 transfected: sc-116124 (B) 293T whole cell lysates.

DDB1 (C-2) - sc-137132 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
DDB1 (C-2): sc-137132. Western blot analysis of DDB1 expression in non-transfected 293T: sc-117752 (A), human DDB1 transfected 293T: sc-116124 (B) and UV irradiated HeLa (C) whole cell lysates.

DDB1 (E-11) - sc-376860 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
DDB1 (E-11): sc-376860. Western blot analysis of DDB1 expression in non-transfected: sc-117752 (A) and human DDB1 transfected: sc-116124 (B) 293T whole cell lysates.

DDB1 (H-300) - sc-25367 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
DDB1 (H-300): sc-25367. Immunofluorescence staining of formalin-fixed HeLa cells showing nuclear localization. Kindly provided by Yang Xiang, Ph.D., Division of Newborn Medicine, Boston Childrens Hospital, Cell Biology Department, Harvard Medical School.

DDB1 (8) - sc-136180 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
DDB1 (8): sc-136180. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

Anti-DDB1 antibody - ab182906 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling DDB1 with ab182906 at 1/25 dilution. The right image has been treated with the immunizing peptide. (Original magnification: ×200)

Anti-DDB1 antibody - ab117254 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab117254, at 5 µg/ml, staining DDB1 in Formalin-fixed, Paraffin-embedded Human Brain cortex tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-DDB1 antibody [1322] - ab13562 from Abcam

Prices $388.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 1322

Anti-DDB1 antibody [EPR6088] - ab124672 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6088
All lanes : Anti-DDB1 antibody [EPR6088] (ab124672) at 1/10000 dilutionLane 1 : HeLa cell lysatesLane 2 : HepG2 cell lysatesLane 3 : NIH 3T3 cell lysatesLane 4 : Jurkat cell lysatesLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution

Anti-DDB1 antibody [EPR6089] - ab109027 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6089
ab109027 stained UV-treated HeLa cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109027 at 1/100 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab150081 used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-DDB1 antibody [EPR6089] (HRP) - ab199026 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6089
All lanes : Anti-DDB1 antibody [EPR6089] (HRP) (ab199026) at 1/5000 dilutionLane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab150035)Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell LysateLane 4 : Placenta (Human) Tissue Lysate - adult normal tissue (ab29745)Lysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

Anti-DDB1 antibody - N-terminal - ab176359 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-DDB1 antibody - N-terminal (ab176359) at 1/1000 dilutionLane 1 : Total cell extracts from HeLaLane 2 : Total cell extracts from JurkatLane 3 : Total cell extracts from T47D

Anti-DDB1 with anti-GAPDH internal loading control antibody - ab75393 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DDB1 with anti-GAPDH internal loading control antibody (ab75393) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Hela Whole Cell Lysate - UV IrradiatedLane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 4 : T47D (Human ductal breast epithelial tumor cell line) Whole Cell Lysate (ab14899)Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 7 : Caco 2 (Human colonic carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-DDB1 Antibody (C-Terminus) - LS-C287347 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human and Mouse DDB1 by Immunohistochemistry. Sample: FFPE section of human pancreatic islet cell tumor (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-DDB1 used at a dilution of 1:1000 (0.2 ug/ml). Detection: DAB.

Anti-DDB1 Antibody (clone 4C5) - LS-C133877 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4C5
Immunoperoxidase of monoclonal antibody to DDB1 on formalin-fixed paraffin-embedded human adrenal gland. [antibody concentration 3 ug/ml]

Anti-DDB1 Antibody - LS-C332277 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry of paraffin-embedded human cervical cancer tissue using DDB1 antibody.

Anti-DDB1 Antibody (C-Terminus) - LS-C286404 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Mouse DDB1 by Immunohistochemistry. Sample: FFPE section of mouse squamous cell carcinoma tumor. Antibody: Affinity purified rabbit anti-DDB1 used at a dilution of 1:250.

Anti-DDB1 Antibody (C-Terminus, clone EPR6089) - LS-C138588 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6089
IHC of paraffin-embedded human breast tissue using anti-DDB1 antibody.

Anti-DDB1 Antibody (Internal) IHC-plus™ - LS-B364 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-DDB1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B364 dilution 1:500.

Anti-DDB1 Antibody (clone 1322) IHC-plus™ - LS-B1691 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 1322
Anti-DDB1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1691 concentration 5 ug/ml.

Anti-DDB1 Antibody (C-Terminus) IHC-plus™ - LS-B1692 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-DDB1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1692 concentration 5 ug/ml.

Anti-DDB1 Antibody (aa1128-1140) IHC-plus™ - LS-B11164 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-DDB1 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11164 concentration 5 ug/ml.
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