DIAPH1

Gene Symbol DIAPH1
Entrez Gene 1729
Alt Symbol DFNA1, DIA1, DRF1, LFHL1, hDIA1
Species Human
Gene Type protein-coding
Description diaphanous-related formin 1
Other Description diaphanous homolog 1|diaphanous-related formin-1|protein diaphanous homolog 1
Swissprots Q59FH8 B7ZKW2 O60610 Q17RN4 A6NF18 Q9UC76 E9PEZ2
Accessions EAW61919 EAW61920 O60610 AB209482 BAD92719 AF051782 AAC05373 AK023345 BAB14533 AK057994 AK094264 AK301195 BAG62775 AK304291 BAG65149 AK307822 AL050086 AY007129 AY360322 AAQ64023 AY363395 AAQ63049 BC007411 AAH07411 BC117257 AAI17258 BC143413 AAI43414 BC143414 BQ945734 BT007318 AAP35982 DA593734 DQ067452 AAZ23039 DQ067453 AAZ23040 XM_005268384 XP_005268441 XM_011537572 XP_011535874 XM_011537573 XP_011535875 NM_001079812 NP_001073280 NM_005219 NP_005210
Function Acts in a Rho-dependent manner to recruit PFY1 to the membrane. Required for the assembly of F-actin structures, such as actin cables and stress fibers. Nucleates actin filaments. Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization. Required for cytokinesis, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration (By similarity). Has neurite outgrowth promoting activity (By similarity). In hear cells, it may play a role in the regulation of actin polymerization in hair cells. The MEMO1-RHOA- DIAPH1 signaling pathway plays an important role in ERBB2- dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membr
Subcellular Location Cell membrane. Cell projection, ruffle membrane. Cytoplasm, cytoskeleton. Note=Membrane ruffles, especially at the tip of ruffles, of motile cells.
Tissue Specificity Expressed in brain, heart, placenta, lung, kidney, pancreas, liver, skeletal muscle and cochlea.
Top Pathways Focal adhesion, Regulation of actin cytoskeleton

Diap1 Antibody - 5486 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using Diap1 Antibody.

Diap1 (E1E4K) Rabbit mAb - 14634 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone E1E4K
Western blot analysis of extracts from various cell lines using Diap1 (E1E4K) Rabbit mAb.

Dia 1 (E-4) - sc-373807 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Dia 1 (E-4): sc-373807. Western blot analysis of Dia 1 expression in HeLa (A) and Nocodozole treated HeLa (B) whole cell lysates.

Dia 1 (C-20) - sc-10885 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Dia 1 (C-20): sc-10885. Western blot analysis of Dia 1 expression in whole cell lysates prepared from untreated (A) and nocodazole-treated (B) HeLa whole cell lysates.

Dia 1 (D-3) - sc-373895 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Dia 1 (D-3): sc-373895. Western blot analysis of Dia 1 expression in HeLa whole cell lysate.

Dia 1 (H-58) - sc-28775 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Dia 1 (H-58): sc-28775. Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebral cortex tissue showing cytoplamic staining of neuronal cells and glial cells.

Dia 1 (N-16) - sc-10888 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Dia 1 (N-16): sc-10888. Western blot analysis of Dia 1 expression in HeLa (A), HeLa + nocodazole (B) and KNRK (C) whole cell lysates.

Dia 1 (V-20) - sc-10886 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Dia 1 (V-20): sc-10886. Immunofluorescence staining of methanol-fixed KNRK cells showing cytoplasmic localization.

Anti-DIAPH1 antibody - ab11173 from Abcam

Prices $394.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling DIAPH1 with ab11173 at 1/1000 (1µg/ml). Detection: DAB.

Anti-DIAPH1 antibody - ab96784 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DIAPH1 antibody (ab96784) at 1/1000 dilutionLane 1 : H1299 whole cell lysateLane 2 : Raji whole cell lysateLysates/proteins at 30 µg per lane.

Anti-DIAPH1 antibody - ab99499 from Abcam

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-DIAPH1 antibody (ab99499) at 0.3 µg/ml (for 1 hour) + NIH3T3 lysate in RIPA buffer at 35 µgdeveloped using the ECL technique

Anti-DIAPH1 antibody - ab11172 from Abcam

Prices $372.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-DIAPH1 antibody - ab103560 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed paraffin-embedded section of Human ovarian carcinoma. ab103560 used at a dilution of 1/100. Detection: DAB staining.

Anti-DIAPH1 antibody - ab171889 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-DIAPH1 antibody (ab171889) at 1 µgLane 1 : DIAPH1 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilutiondeveloped using the ECL technique

Anti-DIAPH1 antibody - ab54629 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab54629 DIAPH1 antibody at 1ug/ml.

Anti-DIAPH1 antibody [EPR7948] - ab129167 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7948
All lanes : Anti-DIAPH1 antibody [EPR7948] (ab129167) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : HeLa cell lysateLane 3 : Human fetal brain lysateLane 4 : K562 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

Anti-DIAPH1 antibody [EPR7949] - ab133683 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7949
All lanes : Anti-DIAPH1 antibody [EPR7949] (ab133683) at 1/1000 dilutionLane 1 : K562 cell lysateLane 2 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-DIAPH1 antibody - C-terminal - ab189840 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DIAPH1 antibody - C-terminal (ab189840) at 1/500 dilutionLane 1 : HL60 cell extractLane 2 : MCF7 cell extractLane 3 : 293T cell extractLane 4 : HepG2 cell extractLane 5 : Mouse liver extractLane 6 : Mouse lung extract

Anti-DIAPH1 Antibody (aa727-765) - LS-C36165 from LifeSpan Bioscience

Prices $560.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-DIAPH1 Antibody - LS-C64716 from LifeSpan Bioscience

Prices $575.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-DIAPH1 Antibody - LS-C64717 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-DIAPH1 Antibody (aa1196-1245) - LS-C151574 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
DIAPH1 antibody Western Blot of HepG2.

Anti-DIAPH1 Antibody (Internal, clone EPR7949) - LS-C154630 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7949
Western blot analysis on (A) K562 and (B) 293T cell lysates using anti-DIAPH1 antibody.

Anti-DIAPH1 Antibody (aa822-832) - LS-C112650 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
DIAPH1 antibody (0.3 ug/ml) staining of NIH3T3 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-DIAPH1 Antibody - LS-C310133 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
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