HDAC2

Gene Symbol HDAC2
Entrez Gene 3066
Alt Symbol HD2, RPD3, YAF1
Species Human
Gene Type protein-coding
Description histone deacetylase 2
Other Description YY1-associated factor 1|transcriptional regulator homolog RPD3
Swissprots Q92769 B4DL58 Q5SRI8 Q5SZ86 E1P561 B3KRS5 Q8NEH4
Accessions EAW48252 EAW48253 EAW48254 EAW48255 Q92769 AA704179 AB209190 BAD92427 AK092156 BAG52487 AK097376 BAG53457 AK296856 BAG59420 AV719958 BC013141 BC031055 AAH31055 BC146376 AAI46377 BC148797 AAI48798 BM550464 BP201509 CN304190 CN315003 U31814 AAC50814 XM_011535786 XP_011534088 XM_011535787 XP_011534089 XM_011535788 XP_011534090 NM_001527 NP_001518 NR_033441 NR_073443
Function Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. May be involved in the transcriptional repression of circadian target genes, such as PER1, mediated by CRY1 through histone deacetylation. Involved in MTA1-m
Subcellular Location Nucleus {ECO:0000269|PubMed:24970816}. Cytoplasm {ECO:0000269|PubMed:24970816}.
Tissue Specificity Widely expressed; lower levels in brain and lung.
Top Pathways Viral carcinogenesis, Alcoholism, Epstein-Barr virus infection, Cell cycle, Huntington's disease

HDAC2 Antibody - 2540 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of various cell types using HDAC2 Antibody.

HDAC2 Antibody (IP Preferred) - 2545 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from 293 and DLD-1 cells using HDAC2 Antibody (IP Preferred).

HDAC2 (3F3) Mouse mAb - 5113 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 3F3
Western blot analysis of extracts from various cell lines using HDAC2 (3F3) Mouse mAb.

HDAC2 (3F3) - sc-81599 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
HDAC2 (3F3): sc-81599. Western blot analysis of HDAC2 expression in K-562 (A), phorbol-treated Jurkat (B), NIH/3T3 (C), HeLa (D) and Hep G2 (E) whole cell lysates.

HDAC2 (B-2) - sc-55541 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
HDAC2 (B-2): sc-55541. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

HDAC2 (C-19) - sc-6296 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
HDAC2 (C-19): sc-6296. Western blot analysis of HDAC2 expression in K-562 (A) and phorbol-induced Jurkat (B) nuclear extracts.

HDAC2 (C-8) - sc-9959 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
HDAC2 (C-8): sc-9959. Western blot analysis of HDAC2 expression in K-562 (A) and NIH/3T3 (B) nuclear extracts.

HDAC2 (F-6) - sc-55542 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
HDAC2 (F-6): sc-55542. Western blot analysis of HDAC2 expression in Jurkat (A) and NIH/3T3 (B) nuclear extracts.

HDAC2 (H-54) - sc-7899 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
HDAC2 (H-54): sc-7899. Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse liver tissue showing nuclear localization.

HDAC2 (HDAC2-62) - sc-56685 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal

p-HDAC2 (Ser 394) - sc-135639 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-HDAC2 (Ser 394): sc-135639. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

Anti-HDAC2 (phospho S394) antibody - ab75602 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-HDAC2 (phospho S394) antibody (ab75602) at 1/500 dilutionLane 1 : Extracts from HT-29 cells (untreated)Lane 2 : Extracts from HT-29 cells treated with UV (20min)

Anti-HDAC2 (phospho S394) antibody - C-terminal - ab139127 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-HDAC2 (phospho S394) antibody - C-terminal (ab139127) at 1/500 dilution + LOVO cells lysates at 30 µg

Anti-HDAC2 antibody - ab16032 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-HDAC2 antibody (ab16032) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell LysateLysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-HDAC2 antibody - ab39669 from Abcam

Prices $379.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-HDAC2 antibody - ab74296 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Staining of formalin-fixed, paraffin-embedded human tonsil tissue using 1/50 ab74296.

Anti-HDAC2 antibody - ab101028 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-HDAC2 antibody (ab101028)Lane 1 : Jurkat lysate at 3 µgLane 2 : Jurkat lysate at 10 µgLane 3 : Jurkat lysate at 30 µg

Anti-HDAC2 antibody - ab90505 from Abcam

Prices $359.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-HDAC2 antibody (ab90505) at 1/1000 dilutionLane 1 : HeLa cell lysate at 3 µgLane 2 : HeLa cell lysate at 10 µgLane 3 : HeLa cell lysate at 30 µg

Anti-HDAC2 antibody - ab136136 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-HDAC2 antibody (ab136136) at 1/1000 dilution (RT for 2 hrs )Lane 1 : Human 293T total cell extract Lane 2 : Mouse thymus total cell extract

Anti-HDAC2 antibody [1194CT1851] - ab188385 from Abcam

Prices $376.00
Sizes 400 µl
Host Mouse
Clonality Monoclonal
Clone 1194CT1851
Anti-HDAC2 antibody [1194CT1851] (ab188385) at 1/1000 dilution + Hela cell lysate at 35 µgdeveloped using the ECL technique

Anti-HDAC2 antibody [EPR5001] - ab124974 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5001
All lanes : Anti-HDAC2 antibody [EPR5001] (ab124974) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : SH-SY5Y cell lysateLane 3 : Jurkat cell lysateLane 4 : A431 cell lysateLysates/proteins at 10 µg per lane.

Anti-HDAC2 antibody, prediluted - ab27499 from Abcam

Prices $376.00
Sizes 7 ml
Host Rabbit
Clonality Polyclonal
Anti-HDAC2 antibody (ab74296) at 1/25 dilution + K562 cell lysate

Anti-HDAC2 antibody [Y461] - ab32117 from Abcam

Prices $404.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone Y461
Overlay histogram showing HeLa cells stained with ab32117 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32117, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Anti-HDAC2 antibody [Y461] (Alexa Fluor® 488) - ab196471 from Abcam

Prices $439.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone Y461
Overlay histogram showing HeLa cells stained with ab196471 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab196471, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-HDAC2 antibody [Y461] (Alexa Fluor® 647) - ab196518 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone Y461
Overlay histogram showing HeLa cells stained with ab196518 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab196518, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 647 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a solid-state 25mW red diode laser (635 nm) and 675/30 bandpass filter.
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