RHOF

Gene Symbol RHOF
Entrez Gene 54509
Alt Symbol ARHF, RIF
Species Human
Gene Type protein-coding
Description ras homolog family member F (in filopodia)
Other Description ras homolog gene family, member F (in filopodia)|rho family GTPase Rif|rho in filopodia|rho-related GTP-binding protein RhoF
Swissprots Q9NXH6 Q8WVB1 Q9HBH0
Accessions CAI61864 EAW98278 EAW98279 Q9HBH0 AF239923 AAG24952 AK000254 BAA91034 AK130459 AK289482 BAF82171 BC018208 AAH18208 BC018721 BC032529 BC043395 BG574506 BM830620 BQ053707 BQ422695 BU527972 CA426077 NM_019034 NP_061907
Function Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Causes the formation of thin, actin-rich surface projections called filopodia. Functions cooperatively with CDC42 and Rac to generate additional structures, increasing the diversity of actin- based morphology.
Subcellular Location Cell membrane {ECO:0000305}; Lipid-anchor {ECO:0000305}; Cytoplasmic side {ECO:0000305}. Cytoplasm, cytoskeleton.

Rho F (S-16) - sc-162092 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Rho F (S-16): sc-162092. Immunoperoxidase staining of formalin fixed, paraffin-embedded human smooth muscle tissue showing cytoplasmic staining of smooth muscle cells.

Rho F (Y-12) - sc-162094 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-RhoF antibody - ab81024 from Abcam

Host Rabbit
Clonality Polyclonal
Immunofluorescence analysis of COS-7 cells expressing rat RhoF-GFP, staining alpha smooth muscle Actin with ab81024. Cells were fixed with paraformaldehyde, permeabilized with 0.2% Saponin and blocked with 10% serum for 10 minutes at 20°C. Samples were incubated with primary antibody (1/200) for 30 minutes at 20°C. An AlexaFluor®568-conjugated goat anti-rabbit polyclonal IgG (1/400) was used as the secondary antibody. See Abreview

Anti-RhoF antibody - ab101349 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
IHC image of RhoF staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab101349, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-RhoF antibody - ab191534 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-RhoF antibody (ab191534)Lane 1 : HeLa whole cell lysateLane 2 : mouse Raw 264.7 whole cell lysateLane 3 : rat H9C2 whole cell lysate

Anti-RhoF antibody - ab169630 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-RhoF antibody (ab169630) at 1 µg/mlLane 1 : RhoF transfected 293T cell lysateLane 2 : 293T cell lysateLysates/proteins at 15 µl per lane.SecondaryGoat anti-Mouse HRP conjugated antibody at 1/2500 dilutiondeveloped using the ECL technique

Anti-RhoF antibody - ab129630 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-RhoF antibody (ab129630) at 1/100 dilution + A375 cell line lysates at 35 µg

Anti-RhoF antibody [1D4B6] - ab201976 from Abcam

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1D4B6
Anti-RhoF antibody [1D4B6] (ab201976) at 1/500 dilution + RhoF recombinant protein

Anti-RHOF Antibody (N-Terminus) - LS-C47030 from LifeSpan Bioscience

Prices $275.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RHOF Antibody (aa130-180) - LS-C94764 from LifeSpan Bioscience

Prices $325.00
Sizes 500 µg
Host Rabbit
Clonality Polyclonal

Anti-RHOF Antibody (aa178-206, AP) - LS-C239976 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RHOF Antibody (aa178-206, APC) - LS-C239978 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RHOF Antibody (aa178-206, Biotin) - LS-C239979 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RHOF Antibody (aa178-206, FITC) - LS-C239980 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RHOF Antibody (aa178-206, PE) - LS-C239981 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RHOF Antibody (aa178-206, Azide-free, HRP) - LS-C247996 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RHOF Antibody (aa177-206) - LS-C324666 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RHOF Antibody - LS-C341030 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RHOF Antibody (aa107-156) - LS-C112026 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
RHOF antibody LS-C112026 Western blot of 293T cell lysate.

Anti-RHOF Antibody (aa178-206) - LS-C162596 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
RHOF Antibody western blot of A375 cell line lysates (35 ug/lane). The RHOF antibody detected the RHOF protein (arrow).

Anti-RHOF Antibody (N-Terminus) - LS-C345539 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of RHOF Antibody with human Lymph Node Tumor lysate.

RhoF Antibody - NBP2-32509 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry: RhoF Antibody [NBP2-32509] - Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.

RhoF Antibody - NBP1-55116 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: RhoF Antibody [NBP1-55116] - 293T cells lysate, concentration 0.2-1 ug/ml.

RhoF Antibody - NBP2-20157 from Novus Biologicals

Prices $349.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: RhoF Antibody [NBP2-20157] - Sample (30 ug of whole cell lysate) A: U87-MG 12% SDS PAGE gel, diluted at 1:500.

RhoF Antibody - NBP1-20190 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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