CHST4

Gene Symbol CHST4
Entrez Gene 10164
Alt Symbol GST3, GlcNAc6ST2, HECGLCNAC6ST, LSST
Species Human
Gene Type protein-coding
Description carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4
Other Description GST-3|HEC-GlcNAc6ST|L-selectin ligand sulfotransferase|N-acetylglucosamine 6-O-sulfotransferase 2|carbohydrate sulfotransferase 4|galactose/N-acetylglucosamine/N-acetylgalactosamine 6-O-sulfotransferase 3|galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3|glcNAc6ST-2|gn6st-2|high endothelial cells N-acetylglucosamine 6-O-sulfotransferase
Swissprots Q9Y5R3 Q8NCG5 Q8IV46
Accessions EAW59235 Q8NCG5 AF131235 AAD33015 AF149783 AAK48417 AF280088 AAG48246 AK026635 AK074746 BAC11177 AL709927 BC035282 AAH35282 DA045171 DA496702 DQ892110 ABM83036 DQ895304 ABM86230 NM_001166395 NP_001159867 NM_005769 NP_005760
Function Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within mucin-associated glycans that ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X on receptors SPN/CD43, GLYCAM1 and MADCAM1. Also involved in biosynthesis of SELL ligand recognized by MECA-79 antibody. Plays a central role in lymphocyte trafficking during chronic inflammation. Has a catalytic preference for core 2- branched mucin-type O-glycans. Can use GlcNAcbeta1-6[Galbeta1- 3]GalNAc-pNP (core 2), GlcNAcbeta1-6ManOMe and GlcNAcbeta1-2Man oligosaccharide structures as acceptors. Has also activity toward core 3 of GlcNAcbeta1-3GalNAc-pNP. Its substrate specificity may be influenced by its subcellular location.
Subcellular Location Golgi apparatus membrane {ECO:0000269|PubMed:12855678}; Single-pass type II membrane protein {ECO:0000269|PubMed:12855678}.
Tissue Specificity Specifically expressed in HEV. Weakly expressed in spleen. Not expressed in other tissues. Expressed in colonic mucinous adenocarcinoma. {ECO:0000269|PubMed:10330415, ECO:0000269|PubMed:11310842, ECO:0000269|PubMed:12107080}.
Top Pathways Glycosaminoglycan biosynthesis - keratan sulfate

HEC-GlcNAc6ST (C-13) - sc-49025 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
HEC-GlcNAc6ST (C-13): sc-49025. Western blot analysis of HEC-GlcNAc6ST expression in IMR-32 (A), HeLa (B) and K-562 (C) whole cell lysates and human adrenal gland tissue extract (D).

HEC-GlcNAc6ST (G-14) - sc-49026 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

HEC-GlcNAc6ST (G-17) - sc-49027 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

HEC-GlcNAc6ST (H-60) - sc-368595 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

HEC-GlcNAc6ST (Q-14) - sc-49031 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-Carbohydrate sulfotransferase 4 antibody - ab28742 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Carbohydrate sulfotransferase 4 antibody (ab28742) at 1.25 µg/ml + Jurkat cell lysateSecondaryHRP conjugated anti-Rabbit IgG 1: 50,000 - 100,000

Anti-CHST4 / GlcNAc6ST2 Antibody - LS-C125248 from LifeSpan Bioscience

Prices $605.00
Sizes 100 µg
Host Sheep
Clonality Polyclonal

Anti-CHST4 / GlcNAc6ST2 Antibody (aa29-386) - LS-C143676 from LifeSpan Bioscience

Prices $465.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-CHST4 / GlcNAc6ST2 Antibody - LS-C121137 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-CHST4 / GlcNAc6ST2 Antibody (aa30-388) - LS-C127216 from LifeSpan Bioscience

Prices $605.00
Sizes 100 µg
Host Sheep
Clonality Polyclonal

Anti-CHST4 / GlcNAc6ST2 Antibody (aa221-320) - LS-C307796 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-CHST4 / GlcNAc6ST2 Antibody (aa156-205) - LS-C30920 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
CHST4 / GST3 antibody LS-C30920 was used in IHC to stain formalin-fixed, paraffin-embedded human lung.

Anti-CHST4 / GlcNAc6ST2 Antibody (aa234-283) IHC-plus™ - LS-B8069 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-GlcNAc6ST2 / CHST4 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8069 dilution 5-10 ug/ml.

Carbohydrate Sulfotransferase 4/CHST4 Antibody - AF5357 from Novus Biologicals

Prices $99.00, $329.00
Sizes 25 µg, 100 µg
Host Sheep
Clonality Polyclonal
Western blot shows lysates of SW13 human adrenal cortex adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Carbohydrate Sulfotransferase 4/CHST4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5357) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Carbohydrate Sulfotransferase 4/CHST4 at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Carbohydrate Sulfotransferase 4/CHST4 Antibody (595223) - MAB5357 from Novus Biologicals

Prices $99.00, $249.00
Sizes 25 µg, 100 µg
Host Mouse
Clonality Monoclonal
Clone 595223

Carbohydrate Sulfotransferase 4/CHST4 Antibody - NBP2-15697 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: Carbohydrate-sulfotransferase-4 Antibody [NBP2-15697] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:1000.

Carbohydrate Sulfotransferase 4/CHST4 Antibody - NBP1-91748 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry: Carbohydrate Sulfotransferase 4/CHST4 Antibody [NBP1-91748] - Staining of human nasopharynx shows strong positivity in subsets of respiratory epithelial cells.

Carbohydrate Sulfotransferase 4/CHST4 Antibody - H00010164-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal

Carbohydrate Sulfotransferase 4/CHST4 Antibody [Biotin] - BAF5357 from Novus Biologicals

Prices $390.00
Sizes 50 µg
Host Sheep
Clonality Polyclonal

Monoclonal Anti-CHST4 antibody produced in mouse - SAB1402502 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4D7
<B>ELISA</B><BR/>Monoclonal Anti-CHST4, antibody produced in mouse, clone 4D7, purified immunoglobulin: <B>Cat. No. SAB1402502</B>: Detection limit for recombinant GST tagged CHST4 is approximately 3 ng/mL.

Anti-CHST4 (AB1) antibody produced in rabbit - AV35786 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Cell Type: HepG2 (<B>Cat. No. AV35786</B>).<BR/><B>Lanes</B><BR/>1. Antibody dilution (concentration) 0.5 μg/mL in cell type HepG2

Anti-CHST4 (AB2) antibody produced in rabbit - AV35787 from Sigma-Aldrich

Prices $267.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-CHST4 (ab2): <B>Cat. No. AV35787</B>: Immunohistochemistry of CHST4 in Human Lung tissue with CHST4 antibody at 4-8 μg/mL.

Anti-CHST4 antibody produced in rabbit - HPA021955 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-CHST4: <B>Cat. No. HPA021955</B>: Immunohistochemical staining of human nasopharynx shows strong positivity in subsets of respiratory epithelial cells.

CHST4 antibody - middle region (ARP35786_P050) - ARP35786_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

CHST4 antibody - middle region (ARP35787_T100) - ARP35787_T100 from Aviva Systems Biology

Prices $229.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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