DLG1

Gene Symbol DLG1
Entrez Gene 1739
Alt Symbol DLGH1, SAP-97, SAP97, dJ1061C18.1.1, hdlg
Species Human
Gene Type protein-coding
Description discs, large homolog 1 (Drosophila)
Other Description disks large homolog 1|presynaptic protein SAP97|synapse-associated protein 97
Swissprots B9EIQ5 B7ZMM0 B4DGZ8 Q12959 E7EWL7 A5YKK7 B4DGU1 Q12958 D3DXB9 E9PG21 D3DXB8
Accessions CCQ43249 EAW53610 EAW53611 EAW53612 EAW53613 EAW53614 Q12959 AA837080 AB209536 BAD92773 AI223994 AK293967 BAG57339 AK294772 BAG57902 AK294855 BAG57959 AK304280 BAG65140 AK310596 AK310893 AK311129 AL831922 CAD38582 BC015560 AAH15560 BC028486 BC042118 BC140841 AAI40842 BC144651 AAI44652 BM728664 EF553524 ABQ66269 U13896 AAA50598 U13897 AAA50599 XM_005269289 XP_005269346 XM_005269301 XP_005269358 XM_011512493 XP_011510795 XM_011512494 XP_011510796 XM_011512495 XP_011510797 XM_011512496 XP_011510798 XM_011512497 XP_011510799 XM_011512498 XP_011510800 XM_011512499 XP_011510801 XM_011512500 XP_011510802 XM_011512501 XP_011510803 XM_011512502 XP_011510804 XM_011512503 XP_011510805 XM_011512504 XP_011510806 XM_011512505 XP_011510807 XM_011512506 XP_011510808 XM_011512507 XP_011510809 XM_011512508 XP_011510810 XM_011512509 XP_011510811 XR_246039 XR_924108 NM_001098424 NP_001091894 NM_001204386 NP_001191315 NM_001204387 NP_001191316 NM_001204388 NP_001191317 NM_001290983 NP_001277912 NM_004087 NP_004078
Function Essential multidomain scaffolding protein required for normal development (By similarity). Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. May play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. Functional regulator of Kv1.5 channel. {ECO:0000250, ECO:0000269|PubMed:10656683, ECO:0000269|PubMed:12445884, ECO:0000269|PubMed:14699157, ECO:0000269|PubMed:15263016, ECO:0000269|PubMed:19213956, ECO:0000269|PubMed:20605917}.
Subcellular Location Membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Basolateral cell membrane {ECO:0000250}. Endoplasmic reticulum membrane {ECO:0000250}. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density {ECO:0000250}. Cell junction, synapse. Cell membrane, sarcolemma. Note=Colocalizes with EPB41 at regions of intercellular contacts. Basolateral in epithelial cells. May also associate with endoplasmic reticulum membranes. Mainly found in neurons soma, moderately found at postsynaptic densities (By similarity). {ECO:0000250}.
Tissue Specificity Abundantly expressed in atrial myocardium (at protein level). Expressed in lung fibroblasts, cervical epithelial and B-cells (at protein level). Widely expressed, with isoforms displaying different expression profiles. {ECO:0000269|PubMed:12445884, ECO:0000269|PubMed:19213956, ECO:0000269|PubMed:7937897}.
Top Pathways T cell receptor signaling pathway, HTLV-I infection, Viral carcinogenesis, Hippo signaling pathway

SAP 97 (D-9) - sc-514478 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SAP 97 (D-9): sc-514478. Western blot analysis of SAP 97 expression in SK-N-SH (A) and SH-SY5Y (B) whole cell lysates.

SAP 97 (S-19) - sc-26532 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
SAP 97 (S-19): sc-26532. Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.

SAP 97 (2D11) - sc-9961 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SAP 97 (2D11):sc-9961. Western blot analysis of SAP 97 expression in MCF7 (A) and HeLa (B) whole cell lysates.

SAP 97 (H-60) - sc-25661 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
SAP 97 (H-60): sc-25661. Western blot analysis of SAP 97 expression in MCF7 (A) and SK-N-SH (B) whole cell lysates.

SAP 97 (N-17) - sc-26530 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
SAP 97 (N-17): sc-26530. Western blot analysis of SAP 97 expression in mouse lung tissue extract.

SAP 97 (P-14) - sc-26533 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
SAP 97 (P-14): sc-26533. Western blot analysis of SAP 97 expression in non-transfected: sc-117752 (A) and mouse SAP 97 transfected: sc-123350 (B) 293T whole cell lysates.

Anti-SAP97 antibody - ab3437 from Abcam

Prices $400.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescent analysis of SAP97 (green) showing staining in the cytoplasm and membrane of NIH-3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3437 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 �C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Anti-SAP97 antibody - ab104681 from Abcam

Host Rabbit
Clonality Polyclonal
ICC/IF image of ab104681 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab104681 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-Rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-SAP97 antibody - ab60551 from Abcam

Prices $376.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SAP97 antibody (ab60551) at 1 µg/mlLane 1 : Thymus (Mouse) Tissue Lysate Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lane 3 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-SAP97 antibody [EPR6821] - ab134156 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6821
All lanes : Anti-SAP97 antibody [EPR6821] (ab134156) at 1/1000 dilutionLane 1 : SH-SY5Y cell lysateLane 2 : HeLa cell lysateLane 3 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-SAP97 antibody [S64-15] - ab189269 from Abcam

Prices $378.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone S64-15
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue, labeling SAP97 using ab189269 at 20 µg/ml.

Anti-SAP97 antibody [S64-15] - ab99042 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone S64-15
Immunohistochemical detection of SAP97 in frozen sections of Mouse brain extract using ab99042.

Anti-SAP97 antibody - N-terminal - ab200158 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-SAP97 antibody - N-terminal (ab200158) at 1/1000 dilutionLane 1 : Mouse brain tissue extractLane 2 : Mouse thymus tissue extractLane 3 : HeLa cell extractLane 4 : Jurkat cell extract

Anti-DLG1 / SAP97 Antibody (aa115-133) - LS-C17750 from LifeSpan Bioscience

Prices $515.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-DLG1 / SAP97 Antibody (N-Terminus) - LS-C17749 from LifeSpan Bioscience

Prices $355.00
Sizes 25 µg
Host Mouse
Clonality Monoclonal

Anti-DLG1 / SAP97 Antibody (aa1-166, clone 2D11) - LS-C35906 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2D11

Anti-DLG1 / SAP97 Antibody (N-Terminus, clone RPI197.4) - LS-C63294 from LifeSpan Bioscience

Prices $295.00, $465.00
Sizes 50 µg, 200 µg
Host Mouse
Clonality Monoclonal
Clone RPI197.4

Anti-DLG1 / SAP97 Antibody (aa115-133) - LS-C93466 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-DLG1 / SAP97 Antibody (clone S64-15) - LS-C153141 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone S64-15

Anti-DLG1 / SAP97 Antibody (aa1-104, clone S64-15, Atto 390) - LS-C228211 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone S64-15

Anti-DLG1 / SAP97 Antibody (clone 1D8) - LS-C197053 from LifeSpan Bioscience

Prices $355.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 1D8

Anti-DLG1 / SAP97 Antibody (aa1-104, clone S64-15, Atto 488) - LS-C228212 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone S64-15

Anti-DLG1 / SAP97 Antibody (aa1-104, clone S64-15, Atto 565) - LS-C228214 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone S64-15

Anti-DLG1 / SAP97 Antibody (Internal, clone EPR6821) - LS-C138977 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6821
Western blot analysis on (A) SH-SY5Y, (B) HeLa, (C) 293T cell lysates using anti-SAP97 antibody.

Anti-DLG1 / SAP97 Antibody (aa661-673) - LS-C87222 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Antibody (0.3 ug/ml) staining of KELLY lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
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