ARF1

Gene Symbol ARF1
Entrez Gene 375
Alt Symbol -
Species Human
Gene Type protein-coding
Description ADP-ribosylation factor 1
Other Description -
Swissprots P84077
Accessions AAA35511 EAW69831 EAW69832 EAW69833 EAW69834 EAW69835 EAW69836 P84077 AF052179 AAC28623 AF055002 AAC09356 AF493881 AAM12595 AK000620 AK057838 AK057875 AK130737 AK293553 BAG57030 AK296020 BAG58790 AK297792 BAG60132 AM393261 CAL38139 AM393462 CAL38339 AM393595 CAL38472 AM393712 CAL38588 BC009247 AAH09247 BC010415 BC010429 AAH10429 BC011358 AAH11358 BF337061 BG421840 BM353046 BM844480 BQ935470 BQ949763 BT007393 AAP36057 M36340 AAA35552 M84326 AAA35512 NM_001024226 NP_001019397 NM_001024227 NP_001019398 NM_001024228 NP_001019399 NM_001658 NP_001649
Function GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasicity of excitatory synapses and spine shrinkage during long-term depression (LTD).
Subcellular Location Golgi apparatus {ECO:0000269|PubMed:17555535}. Cytoplasm, perinuclear region {ECO:0000269|PubMed:17555535}. Cell junction, synapse, synaptosome {ECO:0000250}. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density {ECO:0000250}.
Top Pathways Vibrio cholerae infection, Endocytosis, Legionellosis

ARF (H-50) - sc-9063 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
ARF (H-50) PE: sc-9063 PE. Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: sc-3871.

ARF (C-20) - sc-1870 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

ARF (ARFS 3F1) - sc-53167 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ARF (ARFS 3F1): sc-53167. Western blot analysis of ARF1 expression in non-transfected: sc-117752 (A) and human ARF1 transfected: sc-113571 (B) 293T whole cell lysates.

ARF (N-19) - sc-7622 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

ARF1 (ARFS 1A9/5) - sc-53168 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ARF1 (ARFS 1A9/5): sc-53168. Western blot analysis of ARF1 expression in non-transfected: sc-117752 (A) and human ARF1 transfected: sc-113571 (B) 293T whole cell lysates.

ARF1 (ARFS 5F2/2) - sc-53169 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ARF1 (ARFS 5F2/2): sc-53169. Western blot analysis of ARF1 expression in non-transfected: sc-117752 (A) and mouse ARF1 transfected: sc-124986 (B) 293T whole cell lysates.

Anti-ARF 1+3 antibody - ab91169 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-ARF 1+3 antibody (ab91169) at 1/50 dilution + MCF-7 cell line lysate at 35 µg

Anti-ARF1 antibody - ab76082 from Abcam

Prices $388.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab76082 staining ARF1 in human neuroblastoma cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized for 15 minutes in 0.01% Triton X-100, blocked using 5% serum for 30 minutes at 20°C, then incubated with ab76082 at a 1/100 dilution for 2 hours at 20°C. The secondary used was a Dylight 488 conjugated donkey anti-rabbit IgG (H+L) used at a 1/500 dilution.See Abreview

Anti-ARF1 antibody - ab191376 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-ARF1 antibody (ab191376) at 1/500 dilutionLane 1 : Neuro2A whole cell lysateLane 2 : GL261 whole cell lysateLane 3 : C8D30 whole cell lysateLane 4 : NIH-3T3 whole cell lysateLane 5 : BCL-1 whole cell lysateLane 6 : Raw264.7 whole cell lysateLane 7 : C2C12 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-ARF1 antibody - ab182755 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling ARF1 with ab182755 at 1/25 dilution, in the presence (right panel) or absence (left panel) of immunizing peptide.

Anti-ARF1 antibody - ab96791 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-ARF1 antibody (ab96791) at 1/1000 dilutionLane 1 : A431 whole cell lysateLane 2 : H1299 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-ARF1 antibody - ab96789 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-ARF1 antibody (ab96789) at 1/500 dilution + HepG2 whole cell lysate at 30 µg

Anti-ARF1 antibody [2F7D2] - ab170050 from Abcam

Prices $378.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2F7D2
Anti-ARF1 antibody [2F7D2] (ab170050) at 1/100 dilution + ARF1 Proteins species Human

Anti-ARF1 antibody [ARFS 1A9/5] - ab18108 from Abcam

Prices $378.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone ARFS 1A9/5
ICC/IF image of ab18108 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18108, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-ARF1 antibody [ARFS 3F1] - ab11038 from Abcam

Prices $398.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone ARFS 3F1
ab11038 detecting ARF1 in AGS Human gastric carcinoma cells by sandwich ELISA (detection). Samples were blocked with 5% BSA for 1 hour at 23°C and were incubated with the primary antibody (1/200 in blocking buffer) for 16 hours at 4°C. Anti-mouse HRP (ab6728) (1/1000) was used as the secondary antibody. The Capture antibody was ab436 - a mouse anti-human IgG (20 ug/ml, 4°C overnight)See Abreview

Anti-ARF1 antibody [ARFS 5F2/2] - ab18109 from Abcam

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone ARFS 5F2/2

Anti-ARF1 antibody [EP442Y] - ab32524 from Abcam

Prices $391.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP442Y
Anti-ARF1 antibody [EP442Y] (ab32524) at 1/2000 dilution + 293 cell lysate

Anti-ARF1 antibody [EPR443] - ab108347 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR443
ICC/IF image of ab108347 stained CaCo2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab108347 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-ARF1 Antibody (C-Terminus) - LS-C121112 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-ARF1 Antibody (clone 4G6) - LS-C196684 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4G6

Anti-ARF1 Antibody (C-Terminus, clone ARFS3F1) - LS-C63143 from LifeSpan Bioscience

Prices $305.00, $465.00
Sizes 50 µg, 200 µg
Host Mouse
Clonality Monoclonal
Clone ARFS3F1
Lane 1: MWM, Lane 2: Rat Brain, Lane 3: Mouse Brain, Lane 4: MDCK

Anti-ARF1 Antibody (aa80-106, APC) - LS-C251611 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ARF1 Antibody (aa80-106, FITC) - LS-C251614 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ARF1 Antibody (aa80-106, AP) - LS-C251609 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ARF1 Antibody (aa80-106, Biotin) - LS-C251612 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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