RAG1

Gene Symbol RAG1
Entrez Gene 5896
Alt Symbol RAG-1, RNF74
Species Human
Gene Type protein-coding
Description recombination activating gene 1
Other Description RING finger protein 74|V(D)J recombination-activating protein 1|recombination activating protein 1
Swissprots Q8IY72 Q8NER2 E9PPC4 P15918
Accessions AAG38421 AAM77798 CBX87169 EAW68118 P15918 AY235716 BC037344 AAH37344 M29474 AAA60248 XM_005253041 XP_005253098 XM_011520250 XP_011518552 XM_011520251 XP_011518553 NM_000448 NP_000439
Function Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T- lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'- hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt,
Subcellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00820}.
Tissue Specificity Maturing lymphoid cells.
Top Pathways Primary immunodeficiency

RAG1 (D36B3) Rabbit mAb - 3968 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D36B3
Western blot analysis of extracts from MOLT-4 and HBP-ALL cells using RAG1 (D36B3) Rabbit mAb.

RAG-1 (D-5) - sc-377127 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
RAG-1 (D-5): sc-377127. Western blot analysis of RAG-1 expression in Hs 67 whole cell lysate.

RAG-1 (H-300) - sc-5599 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
RAG-1 (H-300): sc-5599. Immunofluorescence staining of formalin-fixed HeLa cells showing nuclear localization. Kindly provided by Yang Xiang, Ph.D., Division of Newborn Medicine, Boston Childrens Hospital, Cell Biology Department, Harvard Medical School.

RAG-1 (K-20) - sc-363 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
RAG-1 (K-20): sc-363. Western blot analysis of RAG-1 expression in LADMAC nuclear extract.

RAG-1 (P-20) - sc-34269 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

RAG-1 (T-20) - sc-34270 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
RAG-1 (T-20): sc-34270. Western blot analysis of RAG-1 expression in LADMAC nuclear extract.

Anti-RAG1 antibody - ab113821 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-RAG1 antibody (ab113821) at 1 µg/ml + Mouse liver lysate at 10 µg

Anti-RAG1 antibody [EPRAGR1] - ab172637 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPRAGR1
Anti-RAG1 antibody [EPRAGR1] (ab172637) at 1/1000 dilution + Mouse thymus lysate at 10 µg

Anti-RNF74 / RAG1 Antibody (clone 1D9) - LS-C169127 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1D9
Western blot using RAG1 monoclonal antibody against HEK293 (1) and RAG1 (AA: 818-868)-hIgGFc transfected HEK293 (2) cell lysate.

Anti-RNF74 / RAG1 Antibody (Internal) - LS-C210173 from LifeSpan Bioscience

Prices $295.00
Sizes 250 µl
Host Rabbit
Clonality Polyclonal

Anti-RNF74 / RAG1 Antibody (Internal) - LS-C210172 from LifeSpan Bioscience

Prices $295.00
Sizes 250 µl
Host Rabbit
Clonality Polyclonal

Anti-RNF74 / RAG1 Antibody (Internal) - LS-C210171 from LifeSpan Bioscience

Prices $295.00
Sizes 250 µl
Host Rabbit
Clonality Polyclonal

Anti-RNF74 / RAG1 Antibody (aa174-360) - LS-C311825 from LifeSpan Bioscience

Prices $250.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RNF74 / RAG1 Antibody (aa946-995) - LS-C135124 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
RAG1 antibody LS-C135124 Western blot of Mouse Liver lysate. Antibody concentration 1 ug/ml.

RAG1 Antibody (1D9) - NBP2-37449 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1D9
Western Blot: RAG1 Antibody (1D9) [NBP2-37449] - Western blot analysis using RAG1 mAb against HEK293 (1) and RAG1 (AA: 818-868)-hIgGFc transfected HEK293 (2) cell lysate.

RNF74 Polyclonal Antibody - bs-6941R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

CD6-Antibody - AO1759a from Abgent, a WuXi AppTec company

Prices $385.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 6B9
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Rag1 antibody - 70R-8553 from Fitzgerald

Prices $345.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

RAG1 antibody - abx011872 from Abbexa

Host Mouse
Clonality Monoclonal

Anti-RAG1 monoclonal antibody - DCABH-13165 from Creative Diagnostics

Host Rabbit
Clonality Monoclonal

RAG1 Monoclonal Antibody (1D9) - MA5-17163 from Thermo Fisher Scientific

Prices $309.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1D9
Western blot analysis of RAG1 using a RAG1 monoclonal antibody (Product # MA5-17163) against a human RAG1 recombinant protein.

RAG1 Antibody (D36B3) - A16618 from Thermo Fisher Scientific

Host Rabbit
Clonality Monoclonal
Clone D36B3
Western blot analysis of extracts from MOLT-4 and HBP-ALL cells using RAG1 Monoclonal Anti ...

Rag-1 - MBS648489 from MyBioSource

Prices $395.00
Sizes 250 µl
Host Rabbit
Clonality Polyclonal

Rag-1 - MBS646482 from MyBioSource

Prices $395.00
Sizes 250 µl
Host Rabbit
Clonality Polyclonal

Rag-1 - MBS646521 from MyBioSource

Prices $395.00
Sizes 250 µl
Host Rabbit
Clonality Polyclonal
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