RAG1

Gene Symbol	RAG1
Entrez Gene	5896
Alt Symbol	RAG-1, RNF74
Species	Human
Gene Type	protein-coding
Description	recombination activating gene 1
Other Description	RING finger protein 74|V(D)J recombination-activating protein 1|recombination activating protein 1
Swissprots	Q8IY72 Q8NER2 E9PPC4 P15918
Accessions	AAG38421 AAM77798 CBX87169 EAW68118 P15918 AY235716 BC037344 AAH37344 M29474 AAA60248 XM_005253041 XP_005253098 XM_011520250 XP_011518552 XM_011520251 XP_011518553 NM_000448 NP_000439
Function	Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T- lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'- hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt,
Subcellular Location	Nucleus {ECO:0000255|PROSITE- ProRule:PRU00820}.
Tissue Specificity	Maturing lymphoid cells.
Top Pathways	Primary immunodeficiency

• Antibodies
• Assays/Kits
• Proteins/Peptides
• siRNAs/Functional Genomics
• cDNA Clones
• Cell/Tissue/Blood Products

Human RNF74 / RAG1 ELISA Kit (Sandwich ELISA) - LS-F12466 from LifeSpan Bioscience Category ELISA Kit Prices $600.00 Sizes 1 plate Sensitivity 0.156 Range 0.156-10 ng/ml

Recombination Activating Gene 1, RAG-1, Human, ELISA Kit - E-EL-H2319 from ARP American Research Products Category ELISA Kit Prices $522.00 Sizes 96 t Sensitivity 9.375 Range 15.625-1000pg/mL

V(D)J recombination-activating protein 1, RAG1, Human, ELISA Kit - E1550h from ARP American Research Products Category ELISA Kit Prices $596.00 Sizes 96 t Range 0.156-10 ng/mL

Recombination Activating Gene 1, RAG-1, Rabbit, ELISA Kit - E-EL-RB1842 from ARP American Research Products Category ELISA Kit Prices $585.00 Sizes 96 t

Recombination Activating Gene 1, RAG-1, Chicken, ELISA Kit - E-EL-Ch0333 from ARP American Research Products Category ELISA Kit Prices $445.50 Sizes 96 t

Recombination Activating Gene 1, RAG-1, Porcine, ELISA Kit - E-EL-P0990 from ARP American Research Products Category ELISA Kit Prices $585.00 Sizes 96 t

Recombination Activating Gene 1, RAG-1, Sheep, ELISA Kit - E-EL-S0393 from ARP American Research Products Category ELISA Kit Prices $648.00 Sizes 96 t

Recombination Activating Gene 1, RAG-1, Guinea Pig, ELISA Kit - E-EL-GP1159 from ARP American Research Products Category ELISA Kit Prices $522.00 Sizes 96 t

Recombination Activating Gene 1, RAG-1, Monkey, ELISA Kit - E-EL-MK1093 from ARP American Research Products Category ELISA Kit Prices $585.00 Sizes 96 t

Human RAG-1 ELISA Kit - HP0598 from NeoScientific Category ELISA Kit Prices $495.00 Sizes 96 t

Human RAG-1(Recombination Activating Gene 1) ELISA Kit - E-EL-H2319 from Elabscience Category ELISA Kit Sensitivity 9.38 pg/mL Range 15.63—1000 pg/mL

RAG-1, ELISA Kit - MBS2515547 from MyBioSource Category ELISA Kit Prices $410.00, $490.00, $2,040.00, $3,590.00 Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells Sensitivity 9.375pg/mL Range 15.625--1000pg/mL

Human Recombination activating gene 1 ELISA - E01P0591 from BlueGene Biotech Category ELISA Kit Sensitivity 1.0 pg/ml

Pig RNF74 / RAG1 ELISA Kit (Custom ELISA) - LS-F17975 from LifeSpan Bioscience Category ELISA Kit Prices $700.00 Sizes 1 plate Sensitivity To be determined Range 0.156-10 ng/ml

Rabbit RNF74 / RAG1 ELISA Kit - LS-F17977 from LifeSpan Bioscience Category ELISA Kit Prices $700.00 Sizes 1 plate Sensitivity To be determined Range To be determined

Chicken RNF74 / RAG1 ELISA Kit (Custom ELISA) - LS-F17978 from LifeSpan Bioscience Category ELISA Kit Prices $700.00 Sizes 1 plate Sensitivity To be determined Range 0.156-10 ng/ml

Mouse RNF74 / RAG1 ELISA Kit (Custom ELISA) - LS-F17976 from LifeSpan Bioscience Category ELISA Kit Prices $600.00 Sizes 1 plate Sensitivity To be determined Range 0.156-10 ng/ml

V(D)J recombination-activating protein 1, ELISA Kit - MBS288328 from MyBioSource Category ELISA Kit Prices $430.00, $570.00, $2,515.00, $4,520.00 Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells Range 0.31 ng/ml -20.0 ng/ml.

V(D)J recombination-activating protein 1, ELISA Kit - MBS288098 from MyBioSource Category ELISA Kit Prices $380.00, $500.00, $2,170.00, $3,840.00 Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells

V(D)J recombination-activating protein 1, ELISA Kit - MBS288325 from MyBioSource Category ELISA Kit Prices $430.00, $570.00, $2,515.00, $4,520.00 Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells

V(D)J recombination-activating protein 1, ELISA Kit - MBS288537 from MyBioSource Category ELISA Kit Prices $380.00, $500.00, $2,170.00, $3,840.00 Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells Range 0.15 ng/mL -10.0 ng/mL.

Human RAG-1(Recombination Activating Gene 1) ELISA Kit - E-EL-H2319 from Iwai North America Inc. Category ELISA Kit Prices $580.00 Sizes 1 kit Sensitivity 9.38 pg/mLpg/mL Range 15.63~1000 pg/mL

V(D)J recombination-activating protein 1, ELISA Kit - MBS288249 from MyBioSource Category ELISA Kit Prices $490.00, $645.00 Sizes 48 -strip-wells, 96 -strip-wells

Human RAG-1 ELISA Kit - abx054149 from Abbexa Category ELISA Kit Sensitivity 0.01 ng/ml Range 0.07 ng/ml - 5 ng/ml

Human RNF74 / RAG1 ELISA Kit (Sandwich ELISA) - LS-F27172 from LifeSpan Bioscience Category ELISA Kit Prices $870.00 Sizes 1 plate Sensitivity 1 pg/ml Range 0.156-10 ng/ml

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