Gene Symbol | Hcst |
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Entrez Gene | 23900 |
Alt Symbol | DAP10, KAP10 |
Species | Mouse |
Gene Type | protein-coding |
Description | hematopoietic cell signal transducer |
Other Description | DNAX-activation protein 10|membrane protein DAP10|transmembrane adapter protein KAP10 |
Swissprots | Q9R1E7 Q9QUJ0 |
Accessions | AAK52752 EDL24024 EDL24025 Q9QUJ0 AF072846 AAD46988 AF122905 AAD47912 AF172930 AAD50294 AK008005 BAB25404 BC069220 AAH69220 BC145545 AAI45546 BC145824 AAI45825 BC145826 AAI45827 NM_011827 NP_035957 |
Function | Transmembrane adapter protein which associates with KLRK1 to form an activation receptor KLRK1-HCST in lymphoid and myeloid cells; this receptor plays a major role in triggering cytotoxicity against target cells expressing cell surface ligands such as MHC class I chain-related MICA and MICB, and UL16-binding proteins (ULBPs); these ligands are up-regulated by stress conditions and pathological state such as viral infection and tumor transformation. Functions as docking site for PI3-kinase PIK3R1 and GRB2. Interaction of ULBPs with KLRK1-HCST triggers calcium mobilization and activation of the PIK3R1, MAP2K/ERK, and JAK2/STAT5 signaling pathways. Both PIK3R1 and GRB2 are required for full KLRK1-HCST-mediated activation and ultimate killing of target cells. In NK cells, KLRK1-HCST signaling directly induces cytotoxicity and enhances cytokine production initiated via DAP12/TYROBP-associated receptors. In T-cells, it provides primarily costimulation for TCR-induced signals. KLRK1-HCST rece |
Subcellular Location | Membrane {ECO:0000269|PubMed:17100879}; Single-pass type I membrane protein {ECO:0000269|PubMed:17100879}. |
Top Pathways | Natural killer cell mediated cytotoxicity |
DAP10 CRISPR/Cas9 KO Plasmid (m) - sc-423889 from Santa Cruz Biotechnology
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DAP10 HDR Plasmid (m) - sc-423889-HDR from Santa Cruz Biotechnology
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DAP10 Double Nickase Plasmid (m) - sc-423889-NIC from Santa Cruz Biotechnology
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DAP10 Double Nickase Plasmid (m2) - sc-423889-NIC-2 from Santa Cruz Biotechnology
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DAP10 CRISPR Activation Plasmid (m) - sc-423889-ACT from Santa Cruz Biotechnology
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DAP10 CRISPR Activation Plasmid (m2) - sc-423889-ACT-2 from Santa Cruz Biotechnology
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DAP10 Lentiviral Activation Particles (m) - sc-423889-LAC from Santa Cruz Biotechnology
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DAP10 Lentiviral Activation Particles (m2) - sc-423889-LAC-2 from Santa Cruz Biotechnology
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DAP10 siRNA (m) - sc-42853 from Santa Cruz Biotechnology
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DAP10 shRNA Plasmid (m) - sc-42853-SH from Santa Cruz Biotechnology
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DAP10 shRNA (m) Lentiviral Particles - sc-42853-V from Santa Cruz Biotechnology
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CompoZr® Knockout ZFN Kit - CKOZFN33130 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND33130 from Sigma-Aldrich
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HCST siRNA (Mouse) - CRM4573 from Cohesion Biosciences
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HCST - MBS8225602 from MyBioSource
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shRNA set against Mouse Hc (NM_011827.3) - SHH310709 from Creative biogene
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HCST shRNA Plasmid (Mouse) - CHM4573 from Cohesion Biosciences
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