NFATC3

Gene Symbol NFATC3
Entrez Gene 4775
Alt Symbol NFAT4, NFATX
Species Human
Gene Type protein-coding
Description nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3
Other Description NF-ATc3|T cell transcription factor NFAT4|nuclear factor of activated T-cells c3 isoform IE-Xa|nuclear factor of activated T-cells, cytoplasmic 3
Swissprots O75211 Q99840 Q99842 Q14516 Q12968 Q99841
Accessions CAD88555 EAW83210 EAW83211 EAW83212 EAW83213 Q12968 AB209824 BAD93061 AK057574 AK290370 BAF83059 BC001050 AAH01050 BE350846 BU849493 CA418693 DQ895384 ABM86310 EU887605 ACG55625 EU887606 ACG55626 EU887607 ACG55627 EU887608 ACG55628 EU887609 ACG55629 EU887610 ACG55630 EU887611 ACG55631 EU887612 ACG55632 EU887613 ACG55633 EU887614 ACG55634 EU887615 ACG55635 EU887616 ACG55636 EU887617 ACG55637 EU887618 ACG55638 EU887619 ACG55639 EU887620 ACG55640 EU887621 ACG55641 EU887622 ACG55642 EU887623 ACG55643 EU887624 ACG55644 EU887625 ACG55645 L41067 AAA79174 U14510 AAA86308 U85428 AAB46595 U85429 AAB46596 U85430 AAB46597 NM_004555 NP_004546 NM_173163 NP_775186 NM_173165 NP_775188 NM_173164
Function Acts as a regulator of transcriptional activation. Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2. {ECO:0000269|PubMed:18815128}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:18815128}. Nucleus {ECO:0000269|PubMed:18815128}. Note=Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
Tissue Specificity Isoform 1 is predominantly expressed in thymus and is also found in peripheral blood leukocytes and kidney. Isoform 2 is predominantly expressed in skeletal muscle and is also found in thymus, kidney, testis, spleen, prostate, ovary, small intestine, heart, placenta and pancreas. Isoform 3 is expressed in thymus and kidney. Isoform 4 is expressed in thymus and skeletal muscle.
Top Pathways Wnt signaling pathway, HTLV-I infection, MAPK signaling pathway, B cell receptor signaling pathway, T cell receptor signaling pathway

NFAT4 Antibody - 4998 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Jurkat and Ramos cells using NFAT4 Antibody.

NFATc3 (M-75) - sc-8321 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
NFATc3 (M-75): sc-8321. Western blot analysis of NFATc3 isoform expression in Ramos whole cell lysate.

NFATc3 (F-1) - sc-8405 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NFATc3 (F-1): sc-8405. Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic and nuclear staining of cells in tubuli. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

NFATc3 (C-20) - sc-1152 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
NFATc3 (C-20): sc-1152. Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing nuclear staining of cells in glomeruli and nuclear and cytoplasmic staining of cells in tubules.

NFATc3 (A-17) - sc-23814 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

p-NFATc3 (A-1) - sc-365785 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of NFATc3 phosphorylation in untreated (A,B,D,E) and lambda protein phosphatase (sc-200312A) treated (C,F) Jurkat whole cell lysates (A,C,D,F) and Jurkat nuclear extracts (B,E). Antibodies tested include p-NFATc3 (A-1): sc-365785 (A,B,C) and NFATc3 (M-75): sc-8321 (D,E,F).

p-NFATc3 (Ser 171) - sc-68702 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

p-NFATc3 (C-3) - sc-365786 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of NFATc3 phosphorylation in untreated (A,B,D,E) and lambda protein phosphatase (sc-200312A) treated (C,F) Jurkat whole cell lysates (A,D) and Jurkat nuclear extracts (B,C,E,F). Antibodies tested include p-NFATc3 (C-3): sc-365786 (A,B,C) and NFATc3 (F-1): sc-8405 (D,E,F).

p-NFATc3 (Ser 169) - sc-68701 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of NFATc3 phosphorylation in untreated (A,C) and lambda protein phosphatase (sc-200312A) treated (B,D) Jurkat whole cell lysates. Antibodies tested include p-NFATc3 (Ser 169): sc-68701 (A,B) and NFATc3 (M-75): sc-8321 (C,D).

p-NFATc3 (H-2) - sc-365883 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of NFATc3 phosphorylation in untreated (A,C) and lambda protein phosphatase (sc-200312A) treated (B,D) Jurkat whole cell lysates. Antibodies tested include p-NFATc3 (H-2): sc-365883 (A,B) and NFATc3 (M-75): sc-8321 (C,D).

p-NFATc3 (Ser 174) - sc-68704 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal

p-NFATc3 (Ser 240) - sc-68706 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of NFATc3 phosphorylation in untreated (A,B,D,E) and lambda protein phosphatase (sc-200312A) treated (C,F) Jurkat whole cell lysates (A,D) and Jurkat nuclear extracts (B,C,E,F). Antibodies tested include p-NFATc3 (Ser 240): sc-68706 (A,B,C) and NFATc3 (F-1): sc-8405 (D,E,F).

p-NFATc3 (Ser 244) - sc-68707 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

p-NFATc3 (Ser 265) - sc-32982 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of NFATc3 phosphorylation in untreated (A,C) and IL-2-treated (B,D) Jurkat whole cell lysates. Antibodies tested include p-NFATc3 (Ser 265): sc-32982 (A,B) and NFATc3 (M-75): sc-8321 (C,D).

p-NFATc3 (Ser 236) - sc-68705 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

p-NFATc3 (Ser 344) - sc-32983 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of NFATc3 phosphorylation in untreated (A,B,D,E) and lambda protein phosphatase (sc-200312A) treated (C,F) Jurkat whole cell lysates (A,C,D,F) and Jurkat nuclear extracts (B,E). Antibodies tested include p-NFATc3 (Ser 344): sc-32983 (A,B,C) and NFATc3 (M-75): sc-8321 (D,E,F).

p-NFATc3 (Ser 172) - sc-68703 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal

Anti-NFAT4 (phospho S165) antibody - ab59204 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NFAT4 (phospho S165) antibody (ab59204) at 1/500 dilutionLane 1 : HeLa cells treated with calcium (40nM, 30 mins)Lane 2 : HeLa cells treated with calcium (40nM, 30 mins) with immunizing phosphopeptide

Anti-NFAT4 (phospho S165) antibody - ab182649 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NFAT4 (phospho S165) antibody (ab182649) at 1/500 dilutionLane 1 : HeLa cell extractLane 2 : HeLa cell extract with antigen-specific peptide

Anti-NFAT4 (phospho S165) antibody - ab195948 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NFAT4 (phospho S165) antibody (ab195948) at 1/500 dilutionLane 1 : HeLa whole cell lysate with antigen-specific peptideLane 2 : HeLa whole cell lysateLysates/proteins at 12.5 µg per lane.

Anti-NFAT4 antibody - ab93628 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab93628 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab93628 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-NFAT4 antibody - ab83832 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-NFAT4 antibody (ab83832) at 0.2 µg/ml + HeLa cell lysate at 10 µg

Anti-NFAT4 antibody - ab193946 from Abcam

Prices $376.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-NFAT4 antibody (ab193946) at 0.03 µg/ml + Jurkat nuclear lysate (in RIPA buffer) at 35 µgdeveloped using the ECL technique

Anti-NFAT4 antibody - ab59236 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NFAT4 antibody (ab59236) at 1/500 dilutionLane 1 : HeLa cell extracts treated with Ca+ (40nM, 30mins)Lane 2 : HeLa cell extracts treated with Ca+ (40nM, 30mins) and with the Immunising peptide

Anti-NFAT4 antibody [442C2a] - ab54407 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 442C2a
Anti-NFAT4 antibody [442C2a] (ab54407) + immunised recombinant protein
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