ATF2

Gene Symbol ATF2
Entrez Gene 1386
Alt Symbol CRE-BP1, CREB-2, CREB2, HB16, TREB7
Species Human
Gene Type protein-coding
Description activating transcription factor 2
Other Description activating transcription factor 2 splice variant ATF2-var2|cAMP response element-binding protein CRE-BP1|cAMP responsive element binding protein 2, formerly|cAMP-dependent transcription factor ATF-2|cAMP-responsive element-binding protein 2|cyclic AMP-dependent transcription factor ATF-2|cyclic AMP-responsive element-binding protein 2|histone acetyltransferase ATF2
Swissprots A1L3Z2 A4D7V1 D3DPE9 Q8TAR1 A4D7U5 Q53RY2 Q96JT8 P15336 Q13000 G8JLM5 A4D7U4 Q3B7B7 Q4ZFU9
Accessions AAX88876 AAY15004 EAX11109 EAX11110 EAX11111 EAX11112 EAX11113 P15336 AF283776 AI200584 AK128731 BAG54719 AL050192 AY029364 AAK55760 BC026175 AAH26175 BC107698 AAI07699 BC130335 AAI30336 BC130337 AAI30338 BX648469 DA995464 DQ003036 AAY17202 DQ003037 AAY17203 DQ003038 AAY17204 DQ003039 AAY17205 DQ003040 AAY17206 DQ003041 AAY17207 DQ003042 AAY17208 DQ003043 AAY17209 DQ003044 AAY17210 DQ003045 AAY17211 DQ003046 AAY17212 DQ003047 AAY17213 DQ003048 AAY17214 DQ003049 AAY17215 HQ258270 ADR83024 M31630 AAA35951 X15875 CAA33886 NM_001256090 NP_001243019 NM_001256091 NP_001243020 NM_001256092 NP_001243021 NM_001256093 NP_001243022 NM_001256094 NP_001243023 NM_001880 NP_001871 NR_045768 NR_045769 NR_045770 NR_045771 NR_045772 NR_045773 NR_045774
Function Transcriptional activator which regulates the transcription of various genes, including those involved in anti- apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5'-TGACGTCA-3') or to AP-1 (activator protein 1) consensus sequences (5'-TGACTCA-3'). In the nucleus, contributes to global transcription and the DNA damage response, in addition to specific transcriptional activities that are related to cell development, proliferation and death. In the cytoplasm, interacts with and perturbs HK1- and VDAC1-containing complexes at the mitochondrial outer membrane, thereby impairing mitochondrial membrane potential, inducing mitochondrial leakage and promoting cell death. The phosphorylated form (mediated by ATM) plays a role in the DNA damage response and is involved in the ionizing radiation (IR)-induced S phase checkpoint control and in the recruitment of the MRN complex into the IR-induced foci (IRIF).
Subcellular Location Nucleus. Cytoplasm. Mitochondrion outer membrane. Note=Shuttles between the cytoplasm and the nucleus and heterodimerization with JUN is essential for the nuclear localization. Localization to the cytoplasm is observed under conditions of cellular stress and in disease states. Localizes at the mitochondrial outer membrane in response to genotoxic stress. Phosphorylation at Thr-52 is required for its nuclear localization and negatively regulates its mitochondrial localization. Co- localizes with the MRN complex in the IR-induced foci (IRIF).
Tissue Specificity Ubiquitously expressed, with more abundant expression in the brain.
Top Pathways Estrogen signaling pathway, MAPK signaling pathway, Glucagon signaling pathway, Thyroid hormone synthesis, cGMP-PKG signaling pathway

Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb (PE Conjugate) - 13850 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone 11G2
Flow cytometric analysis of Jurkat cells, untreated (blue) or treated with Anisomycin #2222 (green), using Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb (PE Conjugate).

Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb (Biotinylated) - 4036 from Cell Signaling Technology

Host Rabbit
Clonality Monoclonal
Clone 11G2

Phospho-ATF-2 (Thr71) Antibody - 9221 from Cell Signaling Technology

Prices $287.00, $678.00
Sizes 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa, C6 and NIH/3T3 cells, untreated or anisomycin-treated, using Phospho-ATF-2 (Thr71) Antibody.

Phospho-ATF-2 (Thr69/71) Antibody - 9225 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from 293 cells, untreated or UV-treated (lanes 1 and 2), NIH/3T3 cells, untreated or anisomycin-treated (lanes 3 and 4), and C6 cells, untreated or anisomycin-treated (lanes 5 and 6), using Phospho-ATF-2 (Thr69/71) Antibody.

Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb - 5112 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 11G2
Western blot analysis of extracts from untreated or UV-treated COS cells, NIH/3T3 cells and C6 cells, using Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb (upper), or ATF-2 (20F1) Rabbit mAb #9226 (lower).

ATF-2 (20F1) Rabbit mAb - 9226 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 20F1
Western blot analysis of extracts from 293 and NIH/3T3 cells, untreated or UV-treated, using ATF-2 (20F1) Rabbit mAb.

Phospho-ATF-2 (Thr71) Antibody - 5150 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

PhosphoPlus® ATF-2 (Thr71) Antibody Kit - 9220 from Cell Signaling Technology

Clonality Polyclonal
Western blot analysis of extracts from HeLa, C6 and NIH/3T3 cells, untreated or anisomycin-treated, using Phospho-ATF-2 (Thr71) Antibody.

ATF-2 Antibody - 9222 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

ATF-2 (C-19) - sc-187 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
ATF-2 (C-19): sc-187. Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear staining.

ATF-2 (N-96) - sc-6233 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
ATF-2 (N-96): sc-6233. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human tonsil showing nuclear localization.

ATF-2 (F2BR-1) - sc-242 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ATF-2 (F2BR-1): sc-242. Western blot analysis of ATF-2 expression in A-673 nuclear extract.

p-ATF-2 (F-1) - sc-8398 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of ATF-2 phosphorlation in untreated (A,C) and anisomycin induced (B,D) NIH/3T3 cells. Antibodies tested include p-ATF-2 (F-1): sc-8398 (A,B) and ATF-2 (N-96): sc-6233 (C,D).

p-ATF-2 (Thr 71) - sc-7982 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-ATF-2 (Thr 71)-R: sc-7982-R. Western blot analysis of ATF-2 phosphorylation in anisomycin-treated NIH/3T3 cells (A-C). Antibody was preincubated with either the cognate phosphopeptide (B) or unphosphorylated peptide (C).

p-ATF-2 (Ser 112/94) - sc-101636 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-ATF-2 (Ser 112/94): sc-101636. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue extract showing nuclear staining.

p-ATF-2 (Ser 490/Ser 498) - sc-135686 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
p-ATF-2 (Ser 490/Ser 498): sc-135686. Western blot analysis of ATF-2 phosphorylation in human melanoma cells treated with varying doses of the radiomimetic drug neocarzinostatin (NCS).

p-ATF-2 (Ser 62/44) - sc-101635 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-ATF-2 (Ser 62/44): sc-101635. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue extract showing nuclear staining.

p-ATF2 (103C411) - sc-52941 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal

p-ATF-2 (Thr 69) - sc-101637 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-ATF-2 (Thr 69): sc-101637. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue extract showing nuclear staining.

p-ATF-2 (Thr 73/55) - sc-101638 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-ATF-2 (Thr 73/55): sc-101638. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue extract showing nuclear staining.

Anti-ATF2 (phospho S112) antibody - ab195930 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-ATF2 (phospho S112) antibody (ab195930) at 1/500 dilutionLane 1 : MDA-MB-435 whole cell lysate with antigen-specific peptideLane 2 : MDA-MB-435 whole cell lysateLysates/proteins at 12.5 µg per lane.

Anti-ATF2 (phospho S472) antibody - ab194530 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue labeling ATF2 (phospho S472) with ab194530 at 1/50 dilution. The image on the right is treated with blocking peptide.

Anti-ATF2 (phospho S480) antibody - ab62210 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence analysis of HeLa cells, using ATF2 (phospho S480) antibody (ab62210) at 1/500 dilution, in the presence (right) and absence (left) of immunising phosphopeptide.

Anti-ATF2 (phospho S490 + S498) antibody - ab22186 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ATF2 (phospho S490) antibody - ab111482 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab111482, at 1/50 dilution, staining ATF2 in paraffin-embedded Human brain tissue by Immunohistochemistry, in the presence (right panel) or absence (left panel) of immunising peptide.
Search more