PRKACB

Gene Symbol	PRKACB
Entrez Gene	5567
Alt Symbol	PKA C-beta, PKACB
Species	Human
Gene Type	protein-coding
Description	protein kinase, cAMP-dependent, catalytic, beta
Other Description	cAMP-dependent protein kinase catalytic beta subunit isoform 4ab\|cAMP-dependent protein kinase catalytic subunit beta\|protein kinase A catalytic subunit beta
Swissprots	Q96B09 Q59GC0 P22694 Q5BNF3 B1APG4 Q5BNF1 Q5T1K3 Q8IZQ0 Q5BNE9 Q5BNF0 Q7Z3M1 B4DKB0 B4E2Q1 Q14VH1 Q5BNF2 Q8IYR5 Q5CZ92
Accessions	AAN16454 ABG25919 EAW73247 EAW73248 EAW73249 P22694 AA761973 AB209189 BAD92426 AB451239 BAG70053 AB451364 BAG70178 AI445551 AK074904 AK091420 BAG52356 AK296482 BAG59122 AK304322 BAG65172 AK304375 BAG65213 AK314550 BAG37136 AK316115 BAH14486 AL050208 AM392526 CAL37404 AM393233 CAL38111 AM393317 CAL38195 AM393497 CAL38374 AY927364 AAX19487 AY927365 AAX19488 AY927366 AAX19489 AY927367 AAX19490 AY927368 AAX19491 BC016285 AAH16285 BC035058 AAH35058 BI598037 BM905428 BX369882 BX485407 BX537705 CAD97818 BX641026 CAE46017 BX647515 CR936631 CAI56774 DA188681 DA228647 DC322547 DC418574 EU832783 ACE87794 EU832835 ACE87846 EU832837 ACE87848 EU832842 ACE87853 GQ129231 ACT64365 GQ129233 ACT64367 GQ129248 ACT64382 GQ129404 ACT64538 HY160843 M34181 AAA60170 XM_005271015 XP_005271072 XM_005271016 XP_005271073 XM_005271017 XP_005271074 XM_005271018 XP_005271075 XM_005271019 XP_005271076 XM_005271020 XP_005271077 XM_006710758 XP_006710821 XM_011541764 XP_011540066 NM_001242857 NP_001229786 NM_001242858 NP_001229787 NM_001242859 NP_001229788 NM_001242860 NP_001229789 NM_001242861 NP_001229790 NM_001242862 NP_001229791 NM_001300915 NP_001287844 NM_001300916 NP_001287845 NM_001300917 NP_001287846 NM_002731 NP_002722 NM_182948 NP_891993 NM_207578 NP_997461
Function	Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. {ECO:0000269\|PubMed:12420224, ECO:0000269\|PubMed:21423175}.
Subcellular Location	Cytoplasm {ECO:0000269\|PubMed:21423175}. Cell membrane {ECO:0000269\|PubMed:21423175}. Nucleus {ECO:0000250}. Note=Translocates into the nucleus (monomeric catalytic subunit). The inactive holoenzyme is found in the cytoplasm. {ECO:0000250}.
Tissue Specificity	Isoform 1 is most abundant in the brain, with low level expression in kidney. Isoform 2 is predominantly expressed in thymus, spleen and kidney. Isoform 3 and isoform 4 are only expressed in the brain. {ECO:0000269\|PubMed:11589697}.
Top Pathways	Adrenergic signaling in cardiomyocytes, Ovarian steroidogenesis, Wnt signaling pathway, Proteoglycans in cancer, Estrogen signaling pathway

PKAβ cat (C-20) - sc-904 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Cat
Clonality	Polyclonal

PKAβ cat (C-20): sc-904. Western blot analysis of PKAβ catalytic subunit expression in mouse brain extract (A), NIH/3T3 (B), KNRK (C), Mv 1 Lu (D), MDCK (E), MCF7 (F), PC-3 (G) and A549 (H) whole cell lysates.

PKAβ cat (N-17) - sc-30665 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Cat
Clonality	Polyclonal

PKAβ cat (N-17): sc-30665. Western blot analysis of PKAβ cat expression in NIH/3T3 whole cell lysate.

Anti-PKA beta (catalytic subunit) (phospho S338) antibody - ab5816 from Abcam

Prices	$370.00
Sizes	50 µl
Host	Rabbit
Clonality	Polyclonal

Peptide Competition: Lysates prepared from 3T3-L1 cells were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either treated with lambda phosphatase (1) or left untreated (2-5), blocked with a 5% BSA-TBSTbuffer for two hours at room temperature, and incubated with ab5816 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP-conjugate and bands were detected using the Pierce SuperSignalTM method. The data show that the peptide corresponding to PKA cat beta [pS338] blocks the antibody signal, thereby verifying the specificity of the antibody. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.

Anti-PKA beta (catalytic subunit) (phospho T198) antibody - ab118531 from Abcam

Prices	$407.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Human Kidney tissue (formalin-fixed, paraffin-embedded) stained with ab118531 at 5 µg/ml followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-PKA beta (catalytic subunit) antibody - ab187515 from Abcam

Prices	$378.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab187515. Blue DAPI for nuclear staining.

Anti-PKA beta (catalytic subunit) antibody - ab201187 from Abcam

Prices	$359.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PKA beta (catalytic subunit) antibody (ab201187) at 1/1000 dilution + HeLa cell lysate

Anti-PKA beta (catalytic subunit) antibody - ab197047 from Abcam

Prices	$376.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PKA beta (catalytic subunit) antibody (ab197047) at 1/500 dilution + SW480 cell extract

Anti-PKA beta (catalytic subunit) antibody - ab94612 from Abcam

Prices	$376.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PKA beta (catalytic subunit) antibody (ab94612) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal lung lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-PKA beta (catalytic subunit) antibody - ab38233 from Abcam

Prices	$359.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-PKA beta (catalytic subunit) antibody (ab38233) at 1/100 dilution + A375 cell lysate

Anti-PKA beta (catalytic subunit) antibody - ab64801 from Abcam

Prices	$376.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-PKA beta (catalytic subunit) antibody (ab64801) at 1/500 dilutionLane 1 : Extracts from Jurkat cells at 5-30ug with no immunizing peptideLane 2 : Extracts from CoLo205 cells at 5-30ug with no immunizing peptideLane 3 : CoLo205 cells at 5-30ug with immunizing peptide at 5-10ug

Anti-PRKACB Antibody (phospho-Ser338) - LS-C38512 from LifeSpan Bioscience

Prices	$485.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PRKACB Antibody (aa17-47) - LS-C98977 from LifeSpan Bioscience

Prices	$295.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

The anti-PRKACB antibody is used in Western blot to detect PRKACB in A375 cell lysate.

Anti-PRKACB Antibody (C-Terminus) - LS-C111915 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

PRKACB antibody LS-C111915 Western blot of Fetal Heart lysate. Antibody concentration 1 ug/ml.

Anti-PRKACB Antibody (aa291-319, PE) - LS-C267091 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PRKACB Antibody (N-Terminus, APC) - LS-C281372 from LifeSpan Bioscience

Prices	$515.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PRKACB Antibody (N-Terminus, AP) - LS-C281371 from LifeSpan Bioscience

Prices	$515.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PRKACB Antibody (N-Terminus) - LS-C117066 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

PRKACB antibody LS-C117066 Western blot of HCT15 cell lysate.

Anti-PRKACB Antibody (N-Terminus, Biotin) - LS-C281373 from LifeSpan Bioscience

Prices	$515.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PRKACB Antibody (aa291-340) - LS-C118825 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western blot of extracts from Jurkat/COLO205 cells, using KAPCB Antibody. The lane on the right is treated with the synthesized peptide.

Anti-PRKACB Antibody (N-Terminus, Azide-free, HRP) - LS-C281376 from LifeSpan Bioscience

Prices	$515.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PRKACB Antibody (N-Terminus, FITC) - LS-C281377 from LifeSpan Bioscience

Prices	$515.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PRKACB Antibody (N-Terminus, PE) - LS-C281378 from LifeSpan Bioscience

Prices	$515.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PRKACB Antibody (aa291-319, AP) - LS-C284762 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PRKACB Antibody (aa291-319, APC) - LS-C284763 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PRKACB Antibody (aa291-319, Biotin) - LS-C284764 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

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