AKT1

Gene Symbol AKT1
Entrez Gene 207
Alt Symbol AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
Species Human
Gene Type protein-coding
Description v-akt murine thymoma viral oncogene homolog 1
Other Description AKT1m|PKB alpha|RAC-PK-alpha|RAC-alpha serine/threonine-protein kinase|protein kinase B alpha|proto-oncogene c-Akt|rac protein kinase alpha
Swissprots B2RAM5 Q9BWB6 P31749 B7Z5R1
Accessions AAL55732 ABY87524 AGC09587 CAA03677 CAA03712 CDL93491 EAW81881 EAW81882 EAW81883 P31749 AB451242 BAG70056 AB451367 BAG70181 AK094287 AK122894 BAG53786 AK131465 AK299310 BAH12997 AK314256 BAG36922 BC000479 AAH00479 BC001737 AAH01737 BC063408 BC084538 AAH84538 BX647722 BX648205 CN414251 EU832491 ACE87663 EU832571 ACE86973 KF836747 AHA61679 KF836748 AHA61680 KF836749 AHA61681 KF836750 AHA61682 M63167 AAA36539 X61037 CAA43372 XM_005267401 XP_005267458 XM_011536543 XP_011534845 XM_011536544 XP_011534846 NM_001014431 NP_001014431 NM_001014432 NP_001014432 NM_005163 NP_005154
Function AKT1 is one of 3 closely related serine/threonine- protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at 'Ser-50' negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling. Phosphorylation of TBC1D4 triggers the binding of this effector to inhibitory 14-3-3 proteins, which is required for insulin-stimulated glucose transport. AKT regulates also the storage of glucose in the form of glyco
Subcellular Location Cytoplasm. Nucleus. Cell membrane. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus.
Tissue Specificity Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages. {ECO:0000269|PubMed:1718748, ECO:0000269|PubMed:17932490, ECO:0000269|PubMed:20333297}.
Top Pathways Apoptosis, Tight junction, VEGF signaling pathway, Focal adhesion, Toll-like receptor signaling pathway

Phospho-Akt (Thr308) (D25E6) XP ® Rabbit mAb (PE Conjugate) - 13842 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D25E6
Flow cytometric analysis of serum-starved NIH/3T3 cells, untreated (blue) or treated with mouse platelet-derived growth factor BB (200 ng/ml, 10 min; green), using Phospho-Akt (Thr308) (D25E6) XP ® Rabbit mAb (PE Conjugate).

Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb (PE Conjugate) - 5315 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D9E
Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 (PI3 Kinase Inhibitor) #9901, Wortmannin (PI3 Kinase Inhibitor) #9551 and U0126 (MEK1/2 Inhibitor) #9903 (blue), using Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb (PE Conjugate).

Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate) - 4071 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D9E
Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 #9901, Wortmannin #9951 and U0126 #9903 (blue), using Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate).

Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb (APC Conjugate) - 11962 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D9E
Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 #9901, Wortmannin #9951, and U0126 #9903 (blue), using Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb (APC Conjugate).

Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb (Alexa Fluor ® 647 Conjugate) - 4075 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D9E
Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 #9901, wortmannin #9951 and U0126 #9903 (blue), using Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb (Alexa Fluor ® 647 Conjugate).

Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb (Biotinylated) - 5012 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D9E
Western blot analysis of extracts of Jurkat cells, treated with either Calyculin A #9902 or LY294002 #9901, using Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb (Biotinylated) #5012 and detected with Streptavidin-HRP #3999.

Phospho-Akt (Thr308) (C31E5E) Rabbit mAb (Alexa Fluor ® 488 Conjugate) - 2918 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone C31E5E
Flow cytometric analysis of NIH/3T3 cells, untreated (blue) or PDGF-treated (green) using Phospho-Akt (Thr308) (C31E5E) Rabbit mAb (Alexa Fluor® 488 Conjugate).

Phospho-Akt (Thr308) (C31E5E) Rabbit mAb (Alexa Fluor ® 647 Conjugate) - 3375 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone C31E5E
Flow cytometric analysis of NIH-3T3 cells, untreated (blue), or PDGF-treated (green), using Phospho-Akt (Thr308) (C31E5E) Rabbit mAb (Alexa Fluor® 647 Conjugate).

Phospho-Akt (Ser473) (587F11) Mouse mAb (Biotinylated) - 5102 from Cell Signaling Technology

Prices $299.00
Sizes 200 µl (200 immunoprecipitations)
Host Mouse
Clonality Monoclonal
Clone 587F11
Akt phosphorylated at serine 473 was immunoprecipitated from untreated or LY294002 treated Jurkat cell extracts, using Phospho-Akt (Ser473) (587F11) Mouse mAb (Biotinylated). This immunocomplex was then pulled down using streptavidin beads. Western blotting was performed with Phospho-Akt (Ser473) Antibody #9271.

Phospho-Akt (Thr308) (C31E5E) Rabbit mAb (Biotinylated) - 5056 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C31E5E
Western blot analysis of extracts from NIH/3T3 cells, untreated or PDGF-treated, using Phospho-Akt (Thr308) (C31E5E) Rabbit mAb (Biotinylated) and detected using Streptavidin-HRP #3999.

Phospho-Akt (Ser473) (193H12) Rabbit mAb (Alexa Fluor ® 647 Conjugate) - 2337 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone 193H12
Flow cytometric analysis of Jurkat cells, untreated (green) or LY294002 and wortmannin treated (blue), using Phospho-Akt (Ser473) (193H12) Rabbit mAb (Alexa Fluor ® 647 Conjugate).

Phospho-Akt (Ser473) (193H12) Rabbit mAb (Alexa Fluor ® 488 Conjugate) - 2336 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone 193H12
Flow cytometric analysis of Jurkat cells, untreated (green) or LY294002 and wortmannin treated (blue), using Phospho-Akt (Ser473) (193H12) Rabbit mAb (Alexa Fluor ® 488 conjugate).

Phospho-Akt (Thr308) (C31E5E) Rabbit mAb (PE Conjugate) - 9088 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone C31E5E
Flow cytometric analysis of NIH/3T3 cells, treated with Human Platelet-Derived Growth Factor BB (hPDGF-BB) #8912 (green) or treated with LY294002 #9901, Wortmannin #9951, and U0126 #9903 (blue), using Phospho-Akt (Thr308) (C31E5E) Rabbit mAb (PE Conjugate).

Akt (pan) (C67E7) Rabbit mAb (HRP Conjugate) - 8596 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C67E7
Western blot analysis of extracts from various cell lines using Akt (pan) (C67E7) Rabbit mAb (HRP Conjugate).

Akt (5G3) Mouse mAb (Alexa Fluor ® 488 Conjugate) - 2917 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Mouse
Clonality Monoclonal
Clone 5G3
Flow cytometric analysis of Jurkat cells, untreated (left) or or treated with LY294002, wortmannin and U0126 (right), using Akt (5G3) Mouse mAb (Alexa Fluor ® 488 Conjugate) and Phospho-Akt (Ser473) (D9E) Rabbit mAb (Alexa Fluor ® 647Conjugate) #4075.

Akt (5G3) Mouse mAb (Alexa Fluor ® 647 Conjugate) - 2944 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Mouse
Clonality Monoclonal
Clone 5G3
Flow cytometric analysis of Jurkat cells, untreated (left) or or treated with LY294002, wortmannin and U0126 (right), using Akt (5G3) Mouse mAb (Alexa Fluor ® 647 Conjugate) and Phospho-Akt (Ser473) (D9E) Rabbit mAb (Alexa Fluor ® 488Conjugate) #4071.

Akt (pan) (C67E7) Rabbit mAb (Alexa Fluor ® 647 Conjugate) - 5186 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone C67E7
Confocal immunofluorescent analysis of C2C12 cells, treated with LY294002 #9901 (50 μM, 2 hrs; left) or treated with insulin (100 ng/mL, 15 min; right), using Akt (pan) (C67E7) Rabbit mAb (Alexa Fluor ® 647 Conjugate) (red pseudocolor) and Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb (Alexa Fluor ® 488 Conjugate) #9683 (green).

Akt (pan) (C67E7) Rabbit mAb (PE Conjugate) - 8790 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone C67E7
Flow cytometric analysis of Jurkat cells using Akt (pan) (C67E7) Rabbit mAb (PE Conjugate) (green) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (PE Conjugate) #5742 (red).

Akt (pan) (40D4) Mouse mAb (Biotinylated) - 4821 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 40D4
Western blot analysis of extracts from various cell lines using Akt (pan) (40D4) Mouse mAb (Biotinylated).

Akt (pan) (C67E7) Rabbit mAb (Alexa Fluor ® 488 Conjugate) - 5084 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone C67E7
Flow cytometric analysis of Jurkat cells using Akt (pan) (C67E7) Rabbit mAb (Alexa Fluor ® 488 Conjugate) (green) compared to Rabbit (DA1E) mAb IgG XP ® Isotype Control (Alexa Fluor ® 488 Conjugate) #2975 (red).

Akt (pan) (40D4) Mouse mAb (HRP Conjugate) - 4298 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 40D4
Western blot analysis of extracts from various cell lines using Akt (pan) (40D4) Mouse mAb (HRP Conjugate).

Akt (pan) (C67E7) Rabbit mAb (Biotinylated) - 5373 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C67E7
Western blot analysis of extracts from various cell lines using Akt (pan) (C67E7) Rabbit mAb (Biotinylated).

Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb (HRP Conjugate) - 5171 from Cell Signaling Technology

Host Rabbit
Clonality Monoclonal
Clone D9E
Western blot analysis of extracts from NIH/3T3 and Jurkat cells, untreated or treated with either PDGF, Calyculin A or LY294002 #9901 as indicated, using Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb (HRP Conjugate).

Phospho-Akt (Thr308) (244F9) Rabbit mAb (Biotinylated) - 3454 from Cell Signaling Technology

Host Rabbit
Clonality Monoclonal
Clone 244F9

Phospho-Akt (Ser473) (4E2) Mouse mAb (Biotinylated) - 5101 from Cell Signaling Technology

Host Mouse
Clonality Monoclonal
Clone 4E2
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