AKT1

Gene Symbol AKT1
Entrez Gene 207
Alt Symbol AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
Species Human
Gene Type protein-coding
Description v-akt murine thymoma viral oncogene homolog 1
Other Description AKT1m|PKB alpha|RAC-PK-alpha|RAC-alpha serine/threonine-protein kinase|protein kinase B alpha|proto-oncogene c-Akt|rac protein kinase alpha
Swissprots B2RAM5 Q9BWB6 P31749 B7Z5R1
Accessions AAL55732 ABY87524 AGC09587 CAA03677 CAA03712 CDL93491 EAW81881 EAW81882 EAW81883 P31749 AB451242 BAG70056 AB451367 BAG70181 AK094287 AK122894 BAG53786 AK131465 AK299310 BAH12997 AK314256 BAG36922 BC000479 AAH00479 BC001737 AAH01737 BC063408 BC084538 AAH84538 BX647722 BX648205 CN414251 EU832491 ACE87663 EU832571 ACE86973 KF836747 AHA61679 KF836748 AHA61680 KF836749 AHA61681 KF836750 AHA61682 M63167 AAA36539 X61037 CAA43372 XM_005267401 XP_005267458 XM_011536543 XP_011534845 XM_011536544 XP_011534846 NM_001014431 NP_001014431 NM_001014432 NP_001014432 NM_005163 NP_005154
Function AKT1 is one of 3 closely related serine/threonine- protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at 'Ser-50' negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling. Phosphorylation of TBC1D4 triggers the binding of this effector to inhibitory 14-3-3 proteins, which is required for insulin-stimulated glucose transport. AKT regulates also the storage of glucose in the form of glyco
Subcellular Location Cytoplasm. Nucleus. Cell membrane. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus.
Tissue Specificity Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages. {ECO:0000269|PubMed:1718748, ECO:0000269|PubMed:17932490, ECO:0000269|PubMed:20333297}.
Top Pathways Apoptosis, Tight junction, VEGF signaling pathway, Focal adhesion, Toll-like receptor signaling pathway

PathScan ® Phospho-Akt (Thr308) Sandwich ELISA Antibody Pair - 7144 from Cell Signaling Technology

Category Antibody Pair
Prices $459.00
Sizes 1 kit (Reagents for 4 x 96 well plates)
The relationship between lysate protein concentration from untreated and PDGF-treated NIH/3T3 cells and the absorbance at 450 nm using PathScan ® Phospho-Akt (Thr308) Sandwich ELISA Antibody Pair #7144 is shown. After overnight starvation, NIH/3T3 cells were treated with PDGF (50 ng/ml) for 10 minutes at 37ºC and then lysed.

PathScan ® Phospho-Akt1 (Ser473) Chemiluminescent Sandwich ELISA Kit - 7134 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1: Relationship between protein concentration of lysates from untreated and PDGF-treated NIH/3T3 cells and immediate light generation with chemiluminescent substrate is shown. Cells (80% confluence) were treated with PDGF #9909 (50 ng/ml) and lysed after incubation at 37ºC for 20 minutes. Graph inset corresponding to the shaded area shows high sensitivity and a linear response at the low protein concentration range.

PathScan ® Phospho-Akt (Thr308) Chemiluminescent Sandwich ELISA Kit - 7135 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1: Relationship between protein concentration of lysates from untreated and PDGF-treated NIH/3T3 cells and immediate light generation with chemiluminescent substrate is shown. Cells (80% confluence) were treated with PDGF #9909 (50 ng/ml) and lysed after incubation at 37ºC for 5 minutes. Graph inset corresponding to the shaded area shows high sensitivity and a linear response at the low protein concentration range.

PathScan ® Phospho-Akt1 (Ser473) Sandwich ELISA Antibody Pair - 7143 from Cell Signaling Technology

Category Antibody Pair
Prices $459.00
Sizes 1 kit (Reagents for 4 x 96 well plates)
The relationship between lysate protein concentration from untreated and PDGF-treated NIH/3T3 cells and the absorbance at 450 nm using PathScan Phospho-Akt1 (Ser473) Sandwich ELISA Antibody Pair #7143 is shown. After overnight starvation, NIH/3T3 cells were treated with PDGF (50 ng/ml) for 20 minutes at 37ºC and then lysed.

PathScan ® Phospho-Akt (Thr308) Sandwich ELISA Kit - 7252 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1: Treatment of NIH/3T3 cells with PDGF stimulates phosphorylation of Akt at Thr308, detected by PathScan® Phospho-Akt (Thr308) Sandwich ELISA Kit #7252, but does not affect the level of total Akt detected by PathScan® Total Akt1 Sandwich ELISA Kit #7170. OD 450 nm readings are shown in the top figure, while the corresponding Western blots using Phospho-Akt (Thr308) Antibody #9275 (right panel) or Akt Antibody #9272 (left panel), are shown in the bottom figure.

PathScan ® Phospho-Akt1 (Ser473) Sandwich ELISA Kit - 7160 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1: Phospho-Akt1 (Ser473) is detected in untreated Jurkat cells, where as no signal is detected with treatment with LY294002 , a specific PI3 Kinase inhibitor using PathScan ® Phospho-Akt1 (Ser473) Sandwich ELISA Kit #7160.

PathScan ® Total Akt1 Sandwich ELISA Antibody Pair - 7142 from Cell Signaling Technology

Category Antibody Pair
Prices $459.00
Sizes 1 kit (Reagents for 4 x 96 well plates)
The relationship between lysate protein concentration from untreated and PDGF-treated NIH/3T3 cells and the absorbance at 450 nm using PathScan ® Total Akt1 Sandwich ELISA Antibody Pair #7142. After overnight starvation, NIH/3T3 cells were treated with PDGF (50 ng/ml) for 10 minutes at 37ºC and then lysed.

PathScan ® Total Akt1 Chemiluminescent Sandwich ELISA Kit - 7132 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Relationship between protein concentration of lysates from untreated and PDGF-treated NIH/3T3 cells and immediate light generation with chemiluminescent substrate is shown. Cells (80% confluence) were treated with PDGF #9909 (50 ng/ml) and lysed after incubation at 37ºC for 20 minutes. Graph inset corresponding to the shaded area shows high sensitivity and a linear response at the low protein concentration range.

PathScan ® Total Akt1 Sandwich ELISA Kit - 7170 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1: Treatment of NIH/3T3 cells with various concentrations of LY294002 blocks PDGF-stimulated phosphorylation of Akt1 at serine 473 detected by PathScan Phospho-Akt1 (Ser473) Sandwich ELISA kit , #7160, but does not affect the level of total Akt1 protein detected by PathScan Total Akt1 Sandwich ELISA kit #7170. OD450 readings are shown in the top figure, while the corresponding Western blot, using Phospho-Akt (Ser473) Antibody #9271 or Akt Antibody #9272, is shown in the bottom figure.

PathScan ® Akt Signaling Antibody Array Kit (Fluorescent Readout) - 9700 from Cell Signaling Technology

Category Antibody Array
Prices $649.00
Sizes 1 kit (32 multiplexed assays)

PathScan ® Akt Signaling Antibody Array Kit (Chemiluminescent Readout) - 9474 from Cell Signaling Technology

Category Antibody Array
Prices $609.00
Sizes 1 kit (32 multiplexed assays)

PathScan ® EGFR Signaling Antibody Array Kit (Fluorescent Readout) - 12785 from Cell Signaling Technology

Category Antibody Array
Prices $649.00
Sizes 1 kit (32 multiplexed assays)

PathScan ® Immune Cell Signaling Antibody Array Kit (Chemiluminescent Readout) - 13792 from Cell Signaling Technology

Category Antibody Array
Prices $609.00
Sizes 1 kit (32 multiplexed assays)

PathScan ® Stress and Apoptosis Signaling Antibody Array Kit (Chemiluminescent Readout) - 12856 from Cell Signaling Technology

Category Antibody Array
Prices $609.00
Sizes 1 kit (32 multiplexed assays)

PathScan ® Immune Cell Signaling Antibody Array Kit (Fluorescent Readout) - 13788 from Cell Signaling Technology

Category Antibody Array
Prices $649.00
Sizes 1 kit (32 multiplexed assays)

PathScan ® Intracellular Signaling Array Kit (Fluorescent Readout) - 7744 from Cell Signaling Technology

Category ELISA Kit
Prices $649.00
Sizes 1 kit (32 multiplexed assays)

PathScan ® Stress and Apoptosis Signaling Antibody Array Kit (Fluorescent Readout) - 12923 from Cell Signaling Technology

Category Antibody Array
Prices $649.00
Sizes 1 kit (32 multiplexed assays)

PathScan ® EGFR Signaling Antibody Array Kit (Chemiluminescent Readout) - 12622 from Cell Signaling Technology

Category Antibody Array
Prices $609.00
Sizes 1 kit (32 multiplexed assays)

PathScan ® Intracellular Signaling Array Kit (Chemiluminescent Readout) - 7323 from Cell Signaling Technology

Category ELISA Kit
Prices $609.00
Sizes 1 kit (32 multiplexed assays)

PathScan ® Cell Growth 4-Plex Array Kit - 7136 from Cell Signaling Technology

Category ELISA Kit

PathScan ® Signaling Nodes I 4-Plex Array Kit - 7137 from Cell Signaling Technology

Category ELISA Kit

PathScan ® Inflammation 4-Plex Array Kit - 7138 from Cell Signaling Technology

Category ELISA Kit

PathScan ® Signaling Nodes II 4-Plex Array Kit - 7139 from Cell Signaling Technology

Category ELISA Kit

Akt Kinase Assay Kit (Nonradioactive) - 9840 from Cell Signaling Technology

Category Assay Kit
Prices $379.00
Sizes 40 assays
AKT Kinase activity of PDGF-treated NIH/3T3 cell extracts was analyzed by IP/Kinase assay. Cell extracts (200 μl) were incubated overnight with Immobilized Phospho-Akt (Ser473) (D9E) Rabbit mAb #4070. After extensive washing the kinase reaction was performed in the presence of 200 μM of cold ATP and 1 μg of GSK-substrate. Phosphorylation of GSK-3 was measured by Western blot using Phospho-GSK-3 a/β (Ser21/9) Antibody #9327.

HTScan® Akt1 Kinase Assay Kit - 7501 from Cell Signaling Technology

Category Assay Kit
Figure 1. Akt1 kinase activity was measured in a radiometric assay using the following reaction conditions: 5 mM MOPS, pH 7.2, 2.5 mM β-glycerophosphate, 1 mM EGTA, 0.4 mM EDTA, 5 mM MgCl2, 0.05 mM DTT, 50 μM ATP, Substrate: Akt-substrate peptide 200 ng/μL, and variable amount of recombinant Akt1.
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