CHRNB2

Gene Symbol	CHRNB2
Entrez Gene	1141
Alt Symbol	EFNL3, nAChRB2
Species	Human
Gene Type	protein-coding
Description	cholinergic receptor, nicotinic, beta 2 (neuronal)
Other Description	acetylcholine receptor, nicotinic, beta 2 (neuronal)\|beta2 human neuronal nicotinic acetylcholine receptor\|cholinergic receptor, nicotinic, beta polypeptide 2 (neuronal)\|neuronal acetylcholine receptor subunit beta-2\|neuronal nicotinic acetylcholine receptor beta 2
Swissprots	Q9UEH9 P17787
Accessions	AAD45422 CAA05108 CAD88996 EAW53192 P17787 AK313470 BAG36255 BC075040 AAH75040 BC075041 AAH75041 R40506 U62437 AAB40115 X53179 CAA37320 Y08415 CAA69692 NM_000748 NP_000739
Function	After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane permeable to sodiun ions. {ECO:0000269\|PubMed:22361591}.
Subcellular Location	Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
Top Pathways	Nicotine addiction, Neuroactive ligand-receptor interaction

AChRβ2 (H-92) - sc-11372 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

AChRβ2 (H-92): sc-11372. Immunofluorescence staining of methanol-fixed BC<sub>3</sub>H1 cells showing membrane localization.

AChRβ2 (C-20) - sc-1449 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

AChRβ2 (270) - sc-58596 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rat
Clonality	Monoclonal

AChRβ2 (D-23) - sc-130936 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	50 µg/0.5 ml
Host	Rabbit
Clonality	Polyclonal

AChRβ2 (D-23): sc-130936. Western blot analysis of AChRβ2 expression in Daudi whole cell lysate.

Anti-Nicotinic Acetylcholine Receptor beta 2 antibody - ab85693 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

Anti-Nicotinic Acetylcholine Receptor beta 2 antibody (ab85693) at 1 µg/ml + Human cerebellum lysate (in RIPA buffer) at 35 µgdeveloped using the ECL technique

Anti-Nicotinic Acetylcholine Receptor beta 2 antibody - Carboxyterminal end - ab41174 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

ICC/IF image of ab41174 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41174, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-CHRNB2 Antibody (aa155-204) - LS-C31817 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

CHRNB2 antibody LS-C31817 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.

Anti-CHRNB2 Antibody (clone 1C7) - LS-C196917 from LifeSpan Bioscience

Prices	$355.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	1C7

CHRNB2 monoclonal antibody (M01), clone 1C7. Western Blot analysis of CHRNB2 expression in rat testis.

Anti-CHRNB2 Antibody (aa24-244) - LS-C314791 from LifeSpan Bioscience

Prices	$395.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-CHRNB2 Antibody (aa21-70) IHC-plus™ - LS-B937 from LifeSpan Bioscience

Prices	$450.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti-CHRNB2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B937 concentration 10 ug/ml.

Anti-CHRNB2 Antibody (Internal) IHC-plus™ - LS-B8300 from LifeSpan Bioscience

Prices	$395.00
Sizes	50 µg
Host	Goat
Clonality	Polyclonal

Anti-CHRNB2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8300 dilution 3.75 ug/ml.

Anti-CHRNB2 Antibody (aa493-502) - LS-C16853 from LifeSpan Bioscience

Prices	$485.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-CHRNB2 Antibody (C-Terminus) - LS-C73551 from LifeSpan Bioscience

Prices	$415.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-CHRNB2 Antibody (C-Terminus) - LS-C73553 from LifeSpan Bioscience

Prices	$515.00
Sizes	5 ml
Host	Rabbit
Clonality	Polyclonal

Anti-CHRNB2 Antibody (aa21-70) - LS-C31191 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-CHRNB2 Antibody - LS-C125361 from LifeSpan Bioscience

Prices	$465.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-CHRNB2 Antibody - LS-C144727 from LifeSpan Bioscience

Prices	$365.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-CHRNB2 Antibody - LS-C153500 from LifeSpan Bioscience

Prices	$345.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-CHRNB2 Antibody (aa156-205) - LS-C31193 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Nicotinic Acetylcholine Receptor beta 2 Antibody - NBP2-15040 from Novus Biologicals

Prices	$329.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: Nicotinic Acetylcholine Receptor beta 2 Antibody [NBP2-15040] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:5000.

Nicotinic Acetylcholine Receptor beta 2 Antibody - NBP1-28467 from Novus Biologicals

Prices	$299.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

Western Blot: Nicotinic Acetylcholine Receptor beta 2 Antibody [NBP1-28467] - Analysis of Nicotinic Acetylcholine Receptor beta 2 in Human Cerebellum lysate (35ug protein in RIPA buffer) using NBP1-28467 at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Nicotinic Acetylcholine Receptor beta 2 Antibody (1C7) - H00001141-M01 from Novus Biologicals

Prices	$359.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	1C7

CHRNB Polyclonal Antibody - bs-4247R from Bioss

Prices	$269.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti CHRNB2 Polyclonal Antibody, Unconjugated (bs-4247R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Anti-CHRNB2 antibody produced in rabbit - AV13020 from Sigma-Aldrich

Prices	$347.50
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Cell Type: Daudi (<B>Cat. No. AV13020</B>).<BR/><B>Lanes</B><BR/>1. Antibody dilution (concentration) 1.0 μg/mL in cell type Daudi

Anti-CHRNB2 (AB1) antibody produced in rabbit - AV37669 from Sigma-Aldrich

Prices	$267.50
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Cell Type: HepG2 (<B>Cat. No. AV37669</B>).<BR/><B>Lanes</B><BR/>1. Antibody dilution (concentration) 5.0 μg/mL in cell type HepG2

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