FOXD3

Gene Symbol FOXD3
Entrez Gene 27022
Alt Symbol AIS1, Genesis, HFH2, VAMAS2
Species Human
Gene Type protein-coding
Description forkhead box D3
Other Description HNF3/FH transcription factor genesis|forkhead box protein D3
Swissprots Q9UDD1 Q9UJU5 Q9BYM2
Accessions AAF05844 EAX06575 Q9UJU5 AF086235 BC172266 AAI72266 BC172572 AAI72572 L12142 AAK13574 NM_012183 NP_036315
Function Binds to the consensus sequence 5'-A[AT]T[AG]TTTGTTT-3' and acts as a transcriptional repressor. Also acts as a transcriptional activator. Promotes development of neural crest cells from neural tube progenitors. Restricts neural progenitor cells to the neural crest lineage while suppressing interneuron differentiation. Required for maintenance of pluripotent cells in the pre-implantation and peri-implantation stages of embryogenesis. {ECO:0000269|PubMed:11891324}.
Subcellular Location Nucleus {ECO:0000305}.
Tissue Specificity Expressed in chronic myeloid leukemia, Jurkat T-cell leukemia and teratocarcinoma cell lines, but not in any other cell lines or normal tissues examined. {ECO:0000269|PubMed:8499623}.

FoxD3 (D20A9) Rabbit mAb - 2019 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D20A9
Western blot analysis of extracts from HeLa cells, untransfected or transfected with FoxD3, using FoxD3 (D20A9) Rabbit mAb.

FOXD3 (C-20) - sc-27888 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
FOXD3 (C-20): sc-27888. Western blot analysis of FOXD3 expression in non-transfected: sc-117752 (A) and human FOXD3 transfected: sc-372301 (B) 293T whole cell lysates.

FOXD3 (F-23) - sc-133588 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Rabbit
Clonality Polyclonal
FOXD3 (F-23): sc-133588. Western blot analysis of FOXD3 expression in Hep G2 whole cell lysate.

Anti-FOXD3 antibody - ab64807 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-FOXD3 antibody (ab64807) at 1/500 dilutionLane 1 : Extracts of HUVEC cellsLane 2 : Extracts of 293 cellsLane 3 : Extracts of 293 cells, plus immunising peptide at 5-10µgLysates/proteins at 5 µg per lane.

Anti-FOXD3 antibody - ab101449 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-FOXD3 antibody (ab101449) at 1/200 dilutionLane 1 : HUVEC cell lysateLane 2 : HEK293 cell lysate

Anti-FOXD3 antibody [5G9] - ab107248 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5G9
Overlay histogram showing Jurkat cells stained with ab107248 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab107248, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-FOXD3 antibody [5G9] - ab130231 from Abcam

Host Mouse
Clonality Monoclonal
Clone 5G9
All lanes : Anti-FOXD3 antibody [5G9] (ab130231) at 1/500 dilutionLane 1 : NTERA-1 cell lysateLane 2 : HUVE-12 cell lysateLane 3 : HEK293 cell lysateLane 4 : HeLa cell lysateLane 5 : Jurkat cell lysateLane 6 : NIH/3T3 cell lysateLane 7 : K562 cell lysateLane 8 : RAW264.7 cell lysateLane 9 : COS7 cell lysate

Anti-FOXD3 Antibody (aa257-306) IHC-plus™ - LS-B948 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-FOXD3 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B948 concentration 5 ug/ml.

Anti-FOXD3 Antibody - LS-C6945 from LifeSpan Bioscience

Prices $285.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-FOXD3 Antibody - LS-C121216 from LifeSpan Bioscience

Prices $605.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-FOXD3 Antibody (aa51-65) - LS-C148042 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-FOXD3 Antibody (N-Terminus) - LS-C144804 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-FOXD3 Antibody (aa1-140) - LS-C182857 from LifeSpan Bioscience

Prices $650.00
Sizes 100 µg
Host Sheep
Clonality Polyclonal

Anti-FOXD3 Antibody (Internal, clone 15B) - LS-C191734 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Clone 15B

Anti-FOXD3 Antibody - LS-C41164 from LifeSpan Bioscience

Prices $245.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescent microscope analysis of NTERA-2 cells using anti-FOXD3 polyclonal antibody (poly6317) (red). alpha-tubulin (clone 10D8) has been labeled with green and nuclei were stained with DAPI (blue).

Anti-FOXD3 Antibody (clone 5G9) - LS-C169278 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5G9
Western blot using FOXD3 mouse monoclonal antibody against NTERA-1 (1),HUVE-12 (2), HEK293 (3), HeLa (4), Jurkat (5), NIH/3T3 (6), K562 (7), RAW264.7 (8) and COS7 (9) cell lysate.

Anti-FOXD3 Antibody (aa211-260) IHC-plus™ - LS-B7060 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-FOXD3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7060 concentration 10 ug/ml.

Anti-FOXD3 Antibody (Arg233) - LS-C176841 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of FoxD3 (R233) pAb in paraffin-embedded human heart tissue.

Anti-FOXD3 Antibody (clone 5G9) - LS-C169291 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5G9
IHC of paraffin-embedded lung cancer tissues (left) and ovarian cancer tissues (right) using FOXD3 mouse monoclonal antibody with DAB staining.

FoxD3 Antibody - AF2819 from Novus Biologicals

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Sheep
Clonality Polyclonal
Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human FoxD3. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human FoxD3 aa 1-140 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2819) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FoxD3 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

FoxD3 Antibody (5G9) - NBP1-51685 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5G9
Western Blot: FoxD3 Antibody (5G9) [NBP1-51685] - Analysis using FOXD3 mouse mAb against NTERA-2 (1), HUVE-12 (2), HEK293 (3), Hela (4), Jurkat (5), K562 (6), RAW264.7 (7), NIH/3T3 (8), and COS7 (9) cell lysate.

FoxD3 Antibody - NB100-92410 from Novus Biologicals

Prices $349.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: FOXD3 Antibody [NB100-92410] - Western blot (WB) analysis of FOXD3 (R233) antibody  in extracts from HUVEC cells.

FoxD3 Antibody (Poly6317) - NB100-78525 from Novus Biologicals

Prices $259.00
Sizes 50 µl
Host Rabbit
Clonality Monoclonal
Clone Poly6317
Western Blot: FOXD3 Antibody (Poly6317) [NB100-78525] - Whole cell extract from Hela cells (lane 1) and NTERA-2 cells (lane 2) were resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-FOXD3 antibody. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.

FoxD3 Antibody (Poly6317) [Allophycocyanin] - NB100-78525APC from Novus Biologicals

Host Rabbit
Clonality Monoclonal
Clone Poly6317

FoxD3 Antibody (Poly6317) [Alexa Fluor (R) 488] - NB100-78525AF488 from Novus Biologicals

Host Rabbit
Clonality Monoclonal
Clone Poly6317
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