PLD1

Gene Symbol PLD1
Entrez Gene 5337
Alt Symbol -
Species Human
Gene Type protein-coding
Description phospholipase D1, phosphatidylcholine-specific
Other Description choline phosphatase 1|phosphatidylcholine-hydrolyzing phospholipase D1|phospholipase D1
Swissprots Q13393
Accessions EAW78480 Q13393 AB209907 BAD93144 AJ276230 CAB76564 AK026225 AK091897 BC068976 AAH68976 BX648900 DA325526 DA464847 HQ257981 ADR82735 U38545 AAB49031 XM_005247533 XP_005247590 XM_005247534 XP_005247591 XM_011512897 XP_011511199 XM_011512898 XP_011511200 XM_011512899 XP_011511201 XR_924146 NM_001130081 NP_001123553 NM_002662 NP_002653
Function Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic (By similarity). {ECO:0000250}.
Subcellular Location Cytoplasm, perinuclear region {ECO:0000250}. Endoplasmic reticulum membrane {ECO:0000250}; Lipid-anchor {ECO:0000250}; Cytoplasmic side {ECO:0000250}. Golgi apparatus membrane {ECO:0000250}; Lipid-anchor {ECO:0000250}; Cytoplasmic side {ECO:0000250}. Late endosome membrane {ECO:0000250}; Lipid- anchor {ECO:0000250}; Cytoplasmic side {ECO:0000250}.
Tissue Specificity Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine. {ECO:0000269|PubMed:9582313}.
Top Pathways Sphingolipid signaling pathway, Choline metabolism in cancer, cAMP signaling pathway, Glutamatergic synapse, Endocytosis

Phospho-PLD1 (Ser561) Antibody - 3834 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from C6 cells, untreated or PMA-stimulated for the indicated times, using Phospho-PLD1 (Ser561) Antibody (upper) or PLD1 Antibody #3832 (lower).

Phospho-PLD1 (Thr147) Antibody - 3831 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa cells, untreated or PMA-treated for the indicated times, using Phospho-PLD1 (Thr147) Antibody (upper) or PLD1 Antibody #3832 (lower).

PLD1 Antibody - 3832 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa cells stimulated with PMA for 0, 10, 20 and 30 minutes using PLD1 Antibody.

PC-PLD1 (F-12) - sc-28314 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PC-PLD1 (F-12): sc-28314. Western blot analysis of PC-PLD1 expression in MCF7 (A) and HeLa (B) whole cell lysates.

PC-PLD1 (G-13) - sc-48258 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

PC-PLD1 (G-16) - sc-48259 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

PC-PLD1 (H-160) - sc-25512 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PC-PLD1 (H-160): sc-25512. Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.

PC-PLD1 (P-13) - sc-34316 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

PC-PLD1 (S-20) - sc-17847 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PC-PLD1 (S-20): sc-17847. Immunoperoxidase staining of formalin fixed, paraffin-embedded human small intestine tissue showing cytoplasmic staining of glandular cells and Interstitial cells.

PC-PLD1 (C-17) - sc-17848 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PC-PLD1 (C-17): sc-17848. Immunoperoxidase staining of formalin fixed, paraffin-embedded human small intestine tissue showing cytoplasmic staining of glandular cells.

p-PC-PLD1 (Ser 2) - sc-34388 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

p-PC-PLD1 (Ser 561) - sc-34390 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Anti-Phospholipase D antibody - ab189191 from Abcam

Prices $407.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human spleen tissue labeling Phospholipase D with ab189191 at 20 µg/ml.

Anti-Phospholipase D1 (phospho S561) antibody - ab60993 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Human brain tissue stained for Phospholipase D1 with ab60993 at 1/50-1/100 dilution, in the presence (right) or absence (left) of the immunizing phosphopeptide.

Anti-Phospholipase D1 (phospho T147) antibody - ab192801 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Phospholipase D1 (phospho T147) antibody (ab192801) at 1/500 dilutionLane 1 : HepG2 cell extractLane 2 : HepG2 cell extract with antigen-specific peptide

Anti-Phospholipase D1 antibody - ab61125 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab61125 at 1/50 dilution staining Phospholipase D1 in human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence (left) or presence (right) of the immunising peptide.

Anti-Phospholipase D1 antibody - ab10583 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-Phospholipase D1 antibody - ab10585 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-Phospholipase D1 antibody - ab55668 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab55668 Phospholipase D1 antibody at 1ug/ml.

Anti-Phospholipase D1 antibody - ab14986 from Abcam

Prices $370.00
Sizes 5 tests
Host Rabbit
Clonality Polyclonal

Anti-Phospholipase D1 antibody [EP1506Y] - ab68150 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1506Y
Anti-Phospholipase D1 antibody [EP1506Y] (ab68150) at 1/500 dilution + Hela cell lysate at 10 µgSecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-Phospholipase D1 antibody - Aminoterminal end - ab50695 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Phospholipase D1 antibody - Carboxyterminal end - ab50714 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab50714 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab50714, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PLD1 / Phospholipase D1 Antibody (Internal) - LS-C26007 from LifeSpan Bioscience

Prices $515.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PLD1 / Phospholipase D1 Antibody (C-Terminus) - LS-C26055 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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