ALOX12B

Gene Symbol ALOX12B
Entrez Gene 242
Alt Symbol 12R-LOX, ARCI2
Species Human
Gene Type protein-coding
Description arachidonate 12-lipoxygenase, 12R type
Other Description 12R-lipoxygenase|arachidonate 12-lipoxygenase, 12R-type|epidermis-type lipoxygenase 12
Swissprots O75342
Accessions CAC34520 EAW90095 O75342 AF038461 AAC39770 AF059250 AAC79680 BC041058 AAH41058 NM_001139 NP_001130
Function Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12R)- hydroperoxyeicosatetraenoic acid/(12R)-HPETE and minor stereoisomers. In the skin, acts upstream of ALOXE3 on the lineolate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins. Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss. May also play a role in the regulation of the expression of airway mucins. {ECO:0000269|PubMed:21558561, ECO:0000269|PubMed:22441738, ECO:0000269|PubMed:9618483}.
Subcellular Location Cytoplasm {ECO:0000255|PROSITE- ProRule:PRU00726}.
Tissue Specificity Expressed in B-cells, hair follicles, foreskin keratinocytes and adult skin. Also expressed in psoriatic tissue. {ECO:0000269|PubMed:9618483}.
Top Pathways Arachidonic acid metabolism

Epidermis-type 12-LO (C-20) - sc-27362 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Epidermis-type 12-LO (C-20): sc-27362. Western blot analysis of Epidermis-type 12-LO expression in NIH/3T3 whole cell lysate.

Epidermis-type 12-LO (H-95) - sc-98852 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Anti-ALOX12B antibody - ab121785 from Abcam

Prices $443.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab121785 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Anti-ALOX12B antibody - ab194481 from Abcam

Prices $370.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Anti-ALOX12B antibody (ab194481) at 1/500 dilution + Jurkat cell lysate at 50 µg

Anti-ALOX12B Antibody (clone 3G12) - LS-C196647 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3G12

Anti-ALOX12B Antibody (aa622-652, APC) - LS-C212704 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ALOX12B Antibody (aa622-652, AP) - LS-C212702 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ALOX12B Antibody (aa622-652, Biotin) - LS-C212705 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ALOX12B Antibody (aa622-652, FITC) - LS-C212706 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ALOX12B Antibody (aa622-652, Azide-free, HRP) - LS-C212707 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ALOX12B Antibody (aa622-652, PE) - LS-C212708 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ALOX12B Antibody (aa629-660) - LS-C319437 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ALOX12B Antibody (aa622-652) - LS-C156289 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
ALOX12B antibody immunohistochemistry of formalin-fixed and paraffin-embedded human tonsil tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

ALOX12B Antibody - NBP1-89409 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: ALOX12B Antibody [NBP1-89409] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.

ALOX12B Antibody - H00000242-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: ALOX12B Antibody [H00000242-A01] - ALOX12B polyclonal antibody (A01), Lot # 051130JC01 Western Blot analysis of ALOX12B expression in Jurkat ( Cat # L017V1 ).

ALOX12B Antibody (3G12) - H00000242-M01 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3G12

Anti-ALOX12B antibody produced in rabbit - HPA024002 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-ALOX12B: <B>Cat. No. HPA024002</B>: Immunohistochemical staining of human skin shows distinct staining in keratin.

ANTI-ALOX12B(C-TERMINAL) antibody produced in rabbit - SAB1307147 from Sigma-Aldrich

Prices $326.00
Sizes 40 tst
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-ALOX12B (C-term): <B>Cat. No. SAB1307147</B>: ALOX12B Antibody (C-term) western blot analysis in A549 cell line lysates (35 μg/lane).

Monoclonal Anti-ALOX12B antibody produced in mouse - SAB1402687 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3G12

ALOX12B Antibody - C-terminal region (OAAB00105) - OAAB00105 from Aviva Systems Biology

Prices $289.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal

ALOX12B-Antibody-C-term - AP10099b from Abgent, a WuXi AppTec company

Prices $99.00, $295.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
ALOX12B Antibody (C-term) (Cat. #AP10099b) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the ALOX12B antibody detected the ALOX12B protein (arrow).

ALOX12B antibody [C1C3] - GTX106869 from GeneTex

Host Rabbit
Clonality Polyclonal

ALOX12B antibody [N1N2], N-term - GTX114248 from GeneTex

Host Rabbit
Clonality Polyclonal
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