PRKAA1

Gene Symbol PRKAA1
Entrez Gene 5562
Alt Symbol AMPK, AMPKa1
Species Human
Gene Type protein-coding
Description protein kinase, AMP-activated, alpha 1 catalytic subunit
Other Description 5'-AMP-activated protein kinase catalytic subunit alpha-1|5'-AMP-activated protein kinase, catalytic alpha-1 chain|ACACA kinase|AMP -activate kinase alpha 1 subunit|AMP-activated protein kinase, catalytic, alpha-1|AMPK alpha 1|AMPK subunit alpha-1|HMGCR kinase|acetyl-CoA carboxylase kinase|hydroxymethylglutaryl-CoA reductase kinase|tau-protein kinase PRKAA1
Swissprots Q86VS1 Q5D0E1 O00286 Q9UNQ4 A8MTQ6 Q13131 B2R7E1
Accessions EAW56000 EAW56001 Q13131 AB022017 BAA36547 AF100763 AAD43027 AK024252 AK307546 AK312947 BAG35788 BC012622 AAH12622 BC037303 AAH37303 BC048980 AAH48980 BQ049192 BQ716041 BX494823 CD512588 DB236105 JF432366 ADZ15583 U22456 AAA64850 Y12856 CAA73361 XM_006714482 XP_006714545 NM_006251 NP_006242 NM_206907 NP_996790
Function Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphoryl
Subcellular Location Cytoplasm {ECO:0000269|PubMed:15866171}. Nucleus {ECO:0000269|PubMed:15866171}. Note=In response to stress, recruited by p53/TP53 to specific promoters.
Top Pathways Glucagon signaling pathway, AMPK signaling pathway, Oxytocin signaling pathway, Non-alcoholic fatty liver disease (NAFLD), PI3K-Akt signaling pathway

PathScan® Phospho-AMPKα (Thr172) Sandwich ELISA Antibody Pair - 7955 from Cell Signaling Technology

Category Antibody Pair
Prices $459.00
Sizes 1 kit (Reagents for 4 x 96 well plates)
Figure 1. The relationship between the protein concentration of lysates from untreated and H 2 O 2 -treated C2C12 cells and the absorbance at 450 nm using the PathScan ® Phospho-AMPKα (Thr172) Sandwich ELISA Antibody Pair #7955 is shown.

PathScan ® Phospho-AMPKα (Thr172) Chemiluminescent Sandwich ELISA Kit - 7792 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1: Relationship between protein concentration of lysates from phosphatase-treated and H 2 O 2 -treated C2C12 cells and immediate light generation with chemiluminescent substrate is shown. C2C12 cells (80-90% confluent) were treated with H 2 O 2 (10 mM, 10 min, 37ºC). Graph inset corresponding to the shaded area shows high sensitivity and a linear response at the low protein concentration range.

PathScan ® Phospho-AMPKα (Thr172) Sandwich ELISA Kit - 7959 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 2. The relationship between the protein concentration of lysates from untreated, phosphatase-treated and H 2 O 2 -treated C2C12 cells and the absorbance at 450 nm using the PathScan ® Phospho-AMPKα (Thr172) Sandwich ELISA Kit is shown.

PathScan ® Total AMPKα Sandwich ELISA Kit - 7961 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1. AMPKα protein is detected at varied levels from multiple cell lines using the PathScan ® Total AMPKα Sandwich ELISA Kit #7961. The absorbance readings at 450 nm are shown in the top figure, while corresponding western blots using AMPKα (D63G4) Rabbit mAb #5832 are shown in the bottom figure.

AMPK alpha 1 Human SimpleStep ELISA™  Kit - ab181422 from Abcam

Category ELISA Kit
Prices $484.00
Sizes 1 x 96 tests
Sensitivity 0.452 ng/ml
Range 1.6 ng/ml - 100 ng/ml
Background-subtracted data values (mean +/- SD) are graphed. 

AMPKa (pThr172) ELISA Kit - ab154468 from Abcam

Category ELISA Kit
Prices $448.00
Sizes 1 x 96 tests
Sensitivity = 4.5 µg/ml
AMPK α1 ELISA assay - dynamic range in rodent cell lines. Relative levels of AMPK α pT172 can be measured in rodent samples. Left panel shows mOD/min data obtained with C2C12 (mouse myoblasts) serum starved overnight in 5mM glucose DMEM media. Right panel shows H4IIE (rat hepatocarcinoma) serum starved overnight in 5mM glucose DMEM media and treated with 1µM Oligomycin for 3 hours.

AMPKa 1 ELISA Kit - ab140358 from Abcam

Category ELISA Kit
Range 10 ng/ml - 1000 ng/ml

Mouse/Human/Rat PRKAA1 / AMPK Alpha 1 ELISA Kit (Cell-Based ELISA) - LS-F1794 from LifeSpan Bioscience

Category ELISA Kit
Prices $450.00
Sizes 1 plate
Range 0.156-10 ng/ml

Mouse/Human/Rat Phospho-PRKAA1 / AMPK Alpha 1 (Ser485) ELISA Kit (Cell-Based Phosphorylation ELISA) - LS-F1449 from LifeSpan Bioscience

Category ELISA Kit
Prices $550.00
Sizes 1 plate
Range 0.156-10 ng/ml

Human PRKAA1 / AMPK Alpha 1 ELISA Kit (Sandwich ELISA) - LS-F9281 from LifeSpan Bioscience

Category ELISA Kit
Prices $650.00
Sizes 1 plate
Sensitivity 0.061 ng/ml
Range 0.156-10 ng/ml

Human Phospho-AMPK alpha 1 (T183) DuoSet IC ELISA - DYC3528-2 from R&D Systems

Category ELISA Kit
Prices $329.00, $649.00
Sizes 1 kit (for 2 Plates), 1 kit (for 5 Plates)
Range 62.50 - 4,000 pg/mL

Human/Mouse/Rat Total AMPK alpha 1 DuoSet IC ELISA - DYC3197-2 from R&D Systems

Category ELISA Kit
Prices $329.00, $649.00
Sizes 1 kit (for 2 Plates), 1 kit (for 5 Plates)
Range 625.00 - 40,000 pg/mL

Human PRKAa1 ELISA Kit - abx152822 from Abbexa

Category ELISA Kit
Sensitivity 0.062 ng/ml
Range 0.156 ng/ml - 10 ng/ml

5'-AMP-activated protein kinase catalytic subunit alpha-1, PRKAA1, Human, ELISA Kit - E9709h from ARP American Research Products

Category ELISA Kit
Prices $660.00
Sizes 96 t
Range 78-5000 pg/mL

Protein Kinase, AMP Activated Alpha 1, PRKAa1, Human, ELISA Kit - E-EL-H0845 from ARP American Research Products

Category ELISA Kit
Prices $522.00
Sizes 96 t
Sensitivity 46.875
Range 78.125-5000pg/mL

Protein Kinase, AMP Activated Alpha 1, PRKAa1, Mouse, ELISA Kit - E-EL-M0986 from ARP American Research Products

Category ELISA Kit
Prices $585.00
Sizes 96 t

Protein Kinase, AMP Activated Alpha 1, PRKAA1, Rat, ELISA Kit - E-EL-R0822 from ARP American Research Products

Category ELISA Kit
Prices $585.00
Sizes 96 t

Protein Kinase, AMP Activated Alpha 1, PRKAa1, Rabbit, ELISA Kit - E-EL-RB0762 from ARP American Research Products

Category ELISA Kit
Prices $585.00
Sizes 96 t

Protein Kinase, AMP Activated Alpha 1, PRKAa1, Chicken, ELISA Kit - E-EL-Ch2092 from ARP American Research Products

Category ELISA Kit
Prices $522.00
Sizes 96 t

Protein Kinase, AMP Activated Alpha 1, PRKAa1, Porcine, ELISA Kit - E-EL-P1274 from ARP American Research Products

Category ELISA Kit
Prices $585.00
Sizes 96 t

Protein Kinase, AMP Activated Alpha 1, PRKAa1, Sheep, ELISA Kit - E-EL-S0453 from ARP American Research Products

Category ELISA Kit
Prices $648.00
Sizes 96 t

Protein Kinase, AMP Activated Alpha 1, PRKAa1, Guinea Pig, ELISA Kit - E-EL-GP0493 from ARP American Research Products

Category ELISA Kit
Prices $522.00
Sizes 96 t

Protein Kinase, AMP Activated Alpha 1, PRKAa1, Monkey, ELISA Kit - E-EL-MK1856 from ARP American Research Products

Category ELISA Kit
Prices $585.00
Sizes 96 t
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