WB Western Blot



AMPKa 1 ELISA Kit - ab140358 from Abcam

Category ELISA Kit
Range 10 ng/ml - 1000 ng/ml

ApoTrack™ Cytochrome c Apoptosis WB Antibody Cocktail - ab110415 from Abcam

Category Cellular Assay
Prices $448.00
Sizes 180 µg
In this experiment, apoptosis was induced in Jurkat and 143B osteosarcoma cells by FAS and also by treatment with staurosporine (HeLa cells were also treated, but only with STS). Mitochondrial and cytoplasmic fractions were isolated (using kit Cell Fractionation Kit ab109719/MS861) and probed using ab110415 (MSA12). As is clear from the gels, cytochrome c has translocated partially in FAS-induced cells and STS-treated osteosarcoma cells, and almost completely in STS-treated Jurkat and HeLa cells. The three control targets allow for verification of the "cleanness" of the cell fractionation.

Cdc42 Activation Assay Kit - ab173238 from Abcam

Category Cellular Assay
MEF cells were treated with (lane 2) or without EGF (lane 1).Top Panel: Cell lysates were incubated with an anti-active Cdc42 monoclonal antibody. The precipitated active Cdc42 was immunoblotted with the anti-Cdc42 rabbit polyclonal antibody.Bottom Panel: Western blot of the cell lysates used (5% of that used in the top panel) with the anti-Cdc42 rabbit polyclonal antibody provided in the kit.

Complex II WB Antibody Cocktail - ab110410 from Abcam

Category Cellular Assay
Prices $448.00
Sizes 305 µg
All lanes : Complex II WB Antibody Cocktail (ab110410) at 1/250 dilutionLane 1 : human heart mitochondria at 10 µgLane 2 : Immunoprecipitated Complex II at 0.5 µg

Cytochrome c Releasing Apoptosis Assay Kit - ab65311 from Abcam

Category Cellular Assay
Prices $587.00
Sizes 100 tests
5x10e7 Jurkat cells were cultured in the absence (1-2) or presence of 2 uM Camptothecin (CPT) (ab120115) for 24 hours (3-4) or with 10 uM CPT for 4 hours (5-6). 30 uL cytosolic (1, 3, 5) and mitochondrial (2, 4, 6) extracts were loaded onto the gel. Membranes were probed with anti-Cytochrome C Mouse MAb (ab6311) (dilution 1:200) followed by Goat Anti-Mouse IgG (HRP) (ab97040) (dilution 1:2000).Bands were detected at the prediced size of 12 kDa.

Lactate Dehydrogenase B (LDHB) Activity Assay - ab140361 from Abcam

Category Cellular Assay
Prices $448.00
Sizes 1 x 96 tests
Range 8 ng/ml - 500 ng/ml

Membrane Integrity WB Antibody Cocktail - ab110414 from Abcam

Category Cellular Assay
Prices $554.00
Sizes 255 µg
All lanes : Membrane Integrity WB Antibody Cocktail (ab110414)Lane 1 : Rat liver mitos prep #1Lane 2 : Rat liver mitos prep #2Lysates/proteins at 10 µg per lane.

Organelle Detection Western Blot Cocktail - ab133989 from Abcam

Category Cellular Assay
Prices $501.00
Sizes 200 µl
All blocking and antibody incubation steps were done in 5% milk, 20 mM Tris-HCl, 0.1% TWEEN-20. Lane 2-6 : Mouse heart homogenate Whole Tissue Lysate 10 µg Primary antibody: Lane 2 : Anti-Sodium Potassium ATPase antibody – Plasma Membrane Marker Lane 3 : Anti-ATP5A antibody – Mitochondrial Marker Lane 4 : Anti-GAPDH antibody – Cytosolic Marker Lane 5 : Anti-Histone H3 (di methyl K9) antibody – Nuclear Marker Lane 6 : Assembled Organelle Detection CocktailSecondary: ab131368 at 1/1000 dilution.

Pyruvate dehydrogenase (PDH) WB Antibody Cocktail - ab110416 from Abcam

Category Cellular Assay
Prices $416.00, $733.00
Sizes 150 µg, 300 µg
Blocked for overnight with 5% Milk/PBS at 4ºC (or 3 hrs at room temperature).Primary antibody incubatedat 6 µg/mL in 1% milk/PBS for 2hrs at room temperature. Secondary: HRP-conjugated anti-mouse used according to manufacturers instructions. Detection method: ECL+  All wash steps carried out in PBS + 0.05% Tween-20.

Total OXPHOS Blue Native WB Antibody Cocktail - ab110412 from Abcam

Category Cellular Assay
Prices $607.00, $997.00
Sizes 300 µg, 600 µg
Two dimension Blue Native PAGE analysis of fibroblasts that are (A) normal and (B) complex I deficient. It is clear that the complex I deficient cell line shown in B shows no detectable level of complex I. However, all other OXPHOS complexes appear normal including a minor amount of heterocomplex formed between complexes III and IV which is a well documented species. Only a minor mitochondrial enrichment was performed upon these samples cell lines. Each sample represents only 8% of confluent cells taken from a 10 cm diameter tissue culture dish. MWs:(C-I) NDUFA9 - 36kDa(C-II-70) SDHA - 70kDA(C-III-Core 2) UQRC2) - 45kDa(C-IV subunit IV) COX IV - 15kDa(C-V alpha) ATP5A - 55kDa

Total OXPHOS Human WB Antibody Cocktail - ab110411 from Abcam

Category Cellular Assay
Prices $649.00, $997.00
Sizes 360 µg, 720 µg
This image is courtesy of an anonymous AbreviewGel: Non-reduced Denaturing (4-12%)Lanes 1-3: contain 3 individual Human B cell lymphoma samples 50 μg loaded per lane.Blocked for 1 hour with 5% Milk at room temperature.ab110411 used as per instructions.Secondary: HRP-conjugated anti-mouse IgG uesd at 1/300Detection method: ECL+ 2 minutes exposure. 

Total OXPHOS Rodent WB Antibody Cocktail - ab110413 from Abcam

Category Cellular Assay
Prices $626.00
Sizes 300 µg
Figure 1. Rat liver mitochondria labeled with ab110413 (MS604). The sample in lane 1 was kept at room temperature, whereas the remaining three samples were heated to 37°C, 50°C, and 100°C, respectively. This blot shows that boiling of samples leads to a decrease in signal due to aggregation of proteins therefore heating samples at or close to boiling is not recommended.

Ubiquitylation Assay Kit - ab139467 from Abcam

Category Cellular Assay
Prices $180.00
Sizes 20 tests
Western blot of ubiquitin thioester assays for the E1 activating enzyme provided and an example E2, UbcH5a (user supplied). Procedures as described in “Assay Protocol” section. Ubiquitin-enzyme conjugates were detected by Western blotting of E1-Ub (A/B) or E2-Ub (C/D) thioester assays in the presence (A/C) or absence (B/D) of Mg-ATP co-factor, using the Ubiquitin antibody provided, as described in “Western Blot Analysis” section. Results demonstrate the formation of ubiquitin thioester linked E1 and/or E2 conjugates in all +Mg-ATP reactions. The absence of such conjugates in negative control reactions demonstrates that their formation is Mg-ATP dependent (required for E1 activation) and hence derived from the ubiquitin cascade.

APOA5, Antibody Set, BioAssay™ - 029960 from USBiological Life Science
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