Hamster Cricetulus griseus



Using the CREB1 assay kits CREB1 phosphorylation at Ser133 is detected in EGF-treated A431 cells (+), compared with untreated cells (-), whereas no change in total CREB1 levels was observed.
Jurkat cells were harvested by centrifugation, resuspended in HBSS, and seeded at 10K/well (20 µL/well) in a rinsed PhosphoTracer assay plate. The cells were treated with various concentrations of wortmannin (10 µL/well) for 1 h at 37°C, then treated with 5% FCS (10 µL/well) for 15 min. The cells were lysed with 10 µL/well Lysis Mix Concentrate, with shaking for 10 min. Antibody Mix specific for phospho AKT (50 µL/well) was added to the lysate, and the plates were incubated for 1 hr at room temp, with shaking. Plates were washed and Substrate Mix was added. The plates were covered in foil and incubated for 10 min with shaking. Signal in the wells was determined using a plate reader.

AKT 1/2/3 (pS473) PhosphoTracer ELISA Kit - ab119614 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
Sensitivity 10 pg/ml
Range 10 pg/ml - 10000 pg/ml
Using the PhosphoTracer AKT 1/2/3 (pS473) assay, the length of assay incubation time that was required to detect recombinant phospho-AKT was examined. After 15 mins, phospho-AKT concentrations of approximately 100 pg/mL were readily detected, while after 1 hour incubation, the limit of detection was less than 10 pg/mL. Longer incubation times had little effect on overall limit of detection that was observed.

AKT 1/2/3 (pT308) PhosphoTracer ELISA Kit - ab119615 from Abcam

Category ELISA Kit
Sensitivity 50 pg/ml
Range 100 pg/ml - 10000 pg/ml
Detection of AKT 1/2/3 (pT308) in MCF7 cells treated with various concentration of wortmannin, using ab119615.
Using the AKT 1/2/3 (p-Thr308) assay kits or Western blot, AKT 1/2/3 phosphorylation at Thr308 is detected in serum-treated HEK293 cells (+), compared with cells treated with 2 µM wortmannin (-), whereas no change in total AKT 1/2/3 levels was observed.

AKT1 Total PhosphoTracer ELISA Kit - ab119616 from Abcam

Category ELISA Kit
Sensitivity 100 pg/ml
Range 100 pg/ml - 100000 pg/ml
Detection of total AKT1 in MCF7 cells treated with various concentration of wortmannin, using ab119616.

CREB1 (pS133) PhosphoTracer ELISA Kit - ab119623 from Abcam

Category ELISA Kit
Sensitivity 3000 cells/well
Range 3000 cells/well - 100000 cells/well
Detection of CREB1 (pS133) in serum-starved A431 cells treated with varying concentrations of forskolin, using ab119623.
Using the ERK 1/2 assay kits, a significant reduction of ERK 1/2 phosphorylation at Thr202/Tyr204 is detected in A431 cells treated with AG1478 for 3 hours (-), compared with cells treated with EGF for 10 min (+).

ERK 1/2 (pT202/Y204) PhosphoTracer ELISA Kit - ab119625 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
Sensitivity 10 pg/ml
Range 10 pg/ml - 10000 pg/ml
As shown, using the ERK 1/2 assay kits, a significant reduction of ERK 1/2 phosphorylation at Thr202/Tyr204 is detected in A431 cells treated with AG1478 for 3 hours compared with untreated cells, while only a small change in total ERK levels is observed.

ERK 1/2 Total PhosphoTracer ELISA Kit - ab119626 from Abcam

Category ELISA Kit
Sensitivity 100 pg/ml
Range 100 pg/ml - 100000 pg/ml
As shown, using the ERK 1/2 assay kits, a significant reduction of ERK 1/2 phosphorylation at Thr202/Tyr204 is detected in A431 cells treated with AG1478 for 3 hours compared with untreated cells, while only a small change in total ERK levels is observed.

ERK1/2 pThr202/Tyr204 + Total PhosphoTracer ELISA Kit - ab119659 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
PhosphoTracer ERK 1/2 (pT202/Y204) + total ERK ELISA Kit (ab119659) used in Sandwich ELISA.

Estrogen Receptor Alpha ELISA Kit - ab128499 from Abcam

Category ELISA Kit
Prices $740.00
Sizes 1 x 96 tests
Sensitivity > 600 ng/well
Range 600 ng/well - 10000 ng/well

IgG Hamster SimpleStep ELISA™ Kit - ab200010 from Abcam

Category ELISA Kit
Prices $470.00
Sizes 1 x 96 tests
Sensitivity 0.08 ng/ml
Range 0.31 ng/ml - 20 ng/ml
Background-subtracted data values (mean +/- SD) are graphed.

Cell Cytotoxicity Assay Kit (Fluorometric) - ab112119 from Abcam

Category Cellular Assay
Prices $211.00
Sizes 1000 tests
CHO-K1 cell number response was measured with ab112119. CHO-K1 cells at 0 to 10,000 cells/well/100 µL were seeded overnight in a black wall/clear bottom 96-well plate. The cells were incubated with 20 µL/well of Assay Solution for 3 hours at 37 °C. The absorbance intensity was measured at 570 nm and 605 nm. The fluorescence intensity was linear (R2 = 0.998) to the cell number as indicated. The detection limit from the blank controls was 60 cells/well (n=6).

Cell Viability Assay Kit (Fluorometric - Blue) - ab112120 from Abcam

Category Cellular Assay
Prices $211.00
Sizes 5 x 96 tests
CHO-K1 cell number response was measured using ab112120. CHO-K1 cells at 0 to 5,000 cells/well/100 µL were seeded overnight in a black wall/clear bottom 96-well plate. The cells were incubated with 100 µL/well of blue indicator solution for 1 hour at room temperature. The fluorescence intensity was measured at Ex/Em = 405/460 nm. The fluorescence intensity was linear (R2 = 1) to the cell number as indicated. The detection limit was 70 cells/well (n=6).

Cell Viability Assay Kit (Fluorometric - Blue Ex 405 nm) - ab176748 from Abcam

Category Cellular Assay
Prices $206.00
Sizes 500 tests
CHO-K1 cell number response was measured with Cell Viability Assay Kit (Fluorometric - Blue Ex 405 nm) (ab176748). CHO-K1 cells were seeded overnight at 0 - 5,000 cells/well/100 µL in a black wall/clear bottom 96-well plate. The cells were incubated with 100 µL/well of CytoCalcein Violet 450 dye-loading solution for 1 hour at room temperature. The fluorescence intensity was measured at Ex/Em = 405/460 nm. The fluorescence intensity was linear (R2 = 1) to the cell number as indicated.The detection limit was 70 cells/well (n=6).

Cell Viability Assay Kit (Fluorometric - Dual Green/Red) - ab112121 from Abcam

Category Cellular Assay
Prices $278.00
Sizes 2 x 96 tests
CHO-K1 cell number response was measured with ab112121. CHO-K1 cells at 0 to 5,000 cells/well/100 µL were seeded overnight in a black wall/clear bottom 96-well plate. The cells were incubated with 100 µL/well of Green dye-loading solution for 1 hour at 37 °C. The fluorescence intensity was measured at Ex/Em = 490/ 525 nm. The fluorescence intensity was linear (R2 = 1) to the cell number as indicated. The detection limit was 30 cells/well (n=6).

Cell Viability Assay Kit (Fluorometric - Green) - ab112122 from Abcam

Category Cellular Assay
Prices $278.00
Sizes 5 x 96 tests
CHO-K1 cell number response was measured with ab112122. CHO-K1 cells at 0 to 5,000 cells/well/100 µL were seeded overnight in a black wall/clear bottom 96-well plate. The cells were incubated with 100 µL/well of Green dye-loading solution for 1 hour at 37 °C. The fluorescence intensity was measured at Ex/Em = 490/ 525 nm. The fluorescence intensity was linear (R2 = 1) to the cell number as indicated. The detection limit was 30 cells/well (n=6).

Fura-2 No Wash Calcium Assay Kit - ab176766 from Abcam

Category Cellular Assay
Prices $484.00, $3,605.00
Sizes 10 x 96 tests, 100 x 96 tests
CHO-K1 cells were seeded overnight at 40,000 cells/100 μL/well in a Costar black wall/clear bottom 96-well plate. The cells were incubated with 100 μL of the Fura-2 No Wash Calcium Assay Kit (ab176766) for 1 hour at room temperature. ATP (50 μL/well) was added to achieve the final indicated concentrations.

Rabbit anti-MAD2 Antibody, Affinity Purified - PLA0087 from Sigma-Aldrich

Prices $323.50
Sizes 100 µl
<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Rabbit anti-Rad18 Antibody, Affinity Purified - PLA0182 from Sigma-Aldrich

Prices $323.50
Sizes 100 µl
<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).
<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Rabbit anti-SMG1 Antibody, Affinity Purified - PLA0194 from Sigma-Aldrich

Prices $323.50
Sizes 100 µl
<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Rabbit anti-TTI1 Antibody, Affinity Purified - PLA0280 from Sigma-Aldrich

Prices $323.50
Sizes 100 µl
<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Rabbit anti-TAF5 Antibody, Affinity Purified - PLA0288 from Sigma-Aldrich

Prices $323.50
Sizes 100 µl
<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).
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