S. pombe schizosaccharomyces pombe



Anti-Ago1 antibody - ab18190 from Abcam

Prices $382.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Ago1 antibody (ab18190) at 1 µg/ml + S.pombe Whole Cell Lysate at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Anti-ATF1 - S.pombe antibody [ATF1 2A9/8] - ab18123 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone ATF1 2A9/8

Anti-beta Actin antibody [mAbcam 8224] - Loading Control - ab8224 from Abcam

Prices $401.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone mAbcam 8224
IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8224, 3µg/ml overnight at +4°C. A goat anti-mouse HRP-conjugated secondary antibody (ab6789, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Anti-beta Actin antibody [mAbcam 8224] - Loading Control (HRP) - ab197277 from Abcam

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone mAbcam 8224
All lanes : Anti-beta Actin antibody [mAbcam 8224] - Loading Control (HRP) (ab197277) at 1/5000 dilutionLane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell LysateLane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell LysateLane 4 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissueLysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

Anti-beta Tubulin antibody - ab139982 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-beta Tubulin antibody (ab139982) at 1/1000 dilution + S. pombe whole cell extract at 10 µg

Anti-Cdc13 antibody [6F11/2] - ab10873 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 6F11/2

Anti-CDK1 antibody [Y100.4] - ab5467 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone Y100.4

Anti-CEBP Alpha antibody [15C8] - ab15047 from Abcam

Prices $379.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 15C8
Anti-CEBP Alpha antibody [15C8] (ab15047) at 2 µg/ml + 293 cell lysates transfected with CEBP alpha.

Anti-Chp1 antibody - ab28647 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Chp1 antibody (ab28647)Lane 1 : S. pombe chp1Δ lysateLane 2 : S. pombe wildtype lysate

Anti-Chp1 antibody - ChIP Grade - ab18191 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Chp1 antibody - ChIP Grade (ab18191) at 1 µg/mlLane 1 : S. pombe chp1Δ lysateLane 2 : S. pombe wildtype lysate

Anti-Cig2 antibody [CIG 3A11/5] - ab10881 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone CIG 3A11/5
All lanes : Anti-Cig2 antibody [CIG 3A11/5] (ab10881) at 1/1000 dilutionLane 1 : 20ug untreated S. pombe wildtype cells, whole cell lysateLane 2 : 20ug S. pombe wildtype cells 8mM HU treated for 1 hour, whole cell lysateLane 3 : 20ug S. pombe wildtype cells 8mM HU treated for 2 hours, whole cell lysateLane 4 : 20ug S. pombe wildtype cells 8mM HU treated for 3 hours, whole cell lysateSecondaryHRP conjugated sheep anti-mouse antibodydeveloped using the ECL techniqueObserved band size : 50 kDa (why is the actual band size different from the predicted?)Exposure time : 1 minuteThis image is courtesy of an anonymous AbreviewSee Abreview

Anti-cnd2 antibody - ab188277 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-cnd2 antibody (ab188277) at 1/500 dilutionLane 1 : Identification of cnd2 protein in crude cell extract of S. pombeLane 2 : Identification of cnd2-GFP protein in crude cell extract of S. pombe

Anti-Cut15 antibody - ab188250 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Cut15 antibody (ab188250) at 1/1000 dilution + S. pombe crude extract at 10 µg

Anti-dis2 antibody - ab122909 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-dis2 antibody (ab122909) at 1/300 dilutionLane 1 : S.pombe cells wild typeLane 2 : S.pombe cells dis2 deletion mutant

Anti-Dis3 antibody - ab64289 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunfluorescent analysis of S. pombe cells labeling Dis3 with ab64289 (right panel). DAPI stain for chromosomal DNA (left panel).

Anti-EEF1A2 antibody - ab60283 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
EEF1A2 was immunoprecipitated using 0.5mg MCF7 whole cell extract, 5µg of Rabbit polyclonal to EEF1A2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, MCF7 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab60283.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 50kDa; EEF1A2

Anti-Fibrillarin antibody [38F3] - ab4566 from Abcam

Prices $401.00
Sizes 250 µl
Host Mouse
Clonality Monoclonal
Clone 38F3
Rat neurons and glial stained with mouse monoclonal to Fibrillarin (green) and with chicken antibody to neurofilament NF-H (red). Cells were counterstained with a fluorescent DNA probe (blue). Nuclear DNA is revealed with Hoechst dye (blue). Cultures were processed using our standard fixation and staining procedure (in protocol section).

Anti-gamma Tubulin antibody - ab11317 from Abcam

Prices $394.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Ab11317 staining cultured hamster cells (CHO cells) by ICC. The cells were fixed with paraformaldehyde and incubated with ab11317 at a dilution of 1/5000 for 12 hours. A polyclonal goat anti-rabbit antibody conjugated to Alexa Fluor ® 488 was used as the seconday antibody. This image is courtesy of an Abreview submitted by Dr Jan Postberg on 7 December 2005.See Abreview

Anti-GAPDH antibody [GA1R] - ab125247 from Abcam

Prices $379.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone GA1R
All lanes : Anti-GAPDH antibody [GA1R] (ab125247) at 1/2000 dilutionLane 1 : Human tissue lysateLane 2 : Mouse tissue lysateLane 3 : Rat tissue lysateLane 4 : Rabbit tissue lysateLane 5 : Chicken tissue lysateLane 6 : Hamster tissue lysateLysates/proteins at 5 µg per lane.developed using the ECL technique

Anti-GAPDH antibody - Loading Control - ab9485 from Abcam

Prices $403.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab9485 staining GAPDH in HeLa cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab9485 at 5μg/ml and ab7291 at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Rabbit secondary (ab150081) at 2 μg/ml (shown in green) and AlexaFluor®594 Goat anti-Mouse secondary (ab150120) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.Negative controls: 1– Rabbit primary antibody and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.

Anti-Glycerol 3 Phosphate Dehydrogenase antibody - ab87286 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Glycerol 3 Phosphate Dehydrogenase antibody (ab87286) at 1 µg/ml + transfected 293T cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [0.T.57] - ab33021 from Abcam

Prices $373.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 0.T.57

Anti-Histone H2A (phospho S129) antibody - ChIP Grade - ab15083 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All batches of ab15083 are tested in Peptide Array against peptides to different Histone H2A modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H2A - phospho S129 (ab19828), indicating that this antibody specifically recognises the Histone H2A - phospho S129 modification.ab19828 - Histone H2A - phospho S129ab19751 - Histone H2A - unmodified

Anti-Histone H2A (yeast) (phospho S129) antibody - ab17353 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Histone H2A (yeast) (phospho S129) antibody (ab17353) at 1 µg/mlLane 1 : S. pombe lysate (0.1 ug)Lane 2 : S. pombe lysate (0.1 ug) with S. pombe Histone H2A (phospho S129) peptide (ab17576) at 1 µg/mlLane 3 : S. pombe lysate (0.1 ug) with S. pombe Histone H2A (unmodified ) peptide (ab17577) at 1 µg/ml

Anti-Histone H2A antibody - ChIP Grade - ab13923 from Abcam

Prices $403.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab13923 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13923, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) ab150077) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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