S. pombe schizosaccharomyces pombe

Mitochondrial Yeast Isolation Kit - ab178779 from Abcam

Category Cellular Assay
Prices $324.00
Sizes 50 tests
Purified mitochondria were analyzed for intactness by using JC-1 dye, which tests the electrochemical proton gradient (ΔΨM) of the inner mitochondrial membrane. The intact purified mitochondria (control) show aggregation of JC-1 dye, whose signal can be measured at Ex/Em = 530/590 nm. Treatment with antimycin C (100 µM), an inhibitor of the electron transport chain, dissipates the mitochondrial membrane potential resulting in reduced fluorescence signal.
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