Bovine Bos taurus



Bovine Cytokine Antibody Array (10 Targets) - Quantitative - ab197410 from Abcam

Category Antibody Array
Prices $535.00, $905.00, $1,490.00
Sizes 8 tests, 22 tests, 50 tests
Assay Summary for Bovine Cytokine Antibody Array - Quantitative (10 targets) (ab197410).

Complex I Enzyme Activity Dipstick Assay Kit - ab109720 from Abcam

Prices $343.00
Sizes 48 tests
An example using ab109720 to measure Complex I activity in human fibroblast samples. Developed dipsticks from a 1:2 dilution series using a positive control sample and the associated standard curve. Starting material was 30 µg of fibroblast protein extract.

Complex I Human Protein Quantity Dipstick Assay Kit - ab109722 from Abcam

Prices $343.00
Sizes 48 tests
Figure 1. An example using ab109722 to quantify Complex I levels using various concentration of human fibroblast extract.

Complex IV Human Enzyme Activity Dipstick Assay Kit - ab109876 from Abcam

Prices $354.00, $627.00
Sizes 30 tests, 90 tests
Abcam's enzyme activity assays apply a novel approach, whereby target enzymes are first immunocaptured from tissue or cell samples before subsequent functional analysis. Dipstick ELISA Kits extend this concept by utilizing the well-established lateral flow concept, wherein capture antibodies are striped onto nitrocellulose membrane and a wicking pad draws the sample through the antibody bands. All of our ELISA kits utilize highly validated monoclonal antibodies and proprietary buffers, which are able to capture even very large enzyme complexes in their fully-intact, functionally-active states.

Complex IV Human Protein Quantity Dipstick Assay Kit - ab109877 from Abcam

Prices $343.00
Sizes 48 tests
An example using ab109877 to quantify Complex IV levels using various concentration of human fibroblast extract. Shown is a 1:2 dilution series using a positive control sample. Approximately 7 to 8 dipsticks are suitable for covering the entire working range and the blank for background levels. In this example the dilution series starts with 10 µg of fibroblast extract. A one-site hyperbola line was generated for best-fit analysis using GraphPad.

Cytochrome c Profiling ELISA Kit - ab110172 from Abcam

Category ELISA Kit
Prices $448.00
Sizes 96 tests
Principle of Sandwich ELISA used in Cytochrome c Protein Quantity Microplate Assay Kit (ab110172). Substrate plus HRP or AP conjugated to streptavidin provide a colorimetric signal that is readable by any plate readers capable of standard ELISA absorbance measurements.

Cytochrome c Quantitative ELISA - ab154471 from Abcam

Sensitivity = 7.22 ng/ml
Range Sample specific recoverySample typeAverage %RangeCell culture extracts4µg/ml - 100µg/mlTissue Homogenate4µg/ml - 100µg/mlPurified protein6ng/ml - 200ng/ml
A dilution series of Human HepG2 cells extract (A), Rat Heart Homogenate extract (B), and Mouse Liver Homogenate extract (C).

Endothelin 1 ELISA Kit - ab133030 from Abcam

Category ELISA Kit
Prices $625.00
Sizes 1 x 96 tests
Sensitivity 0.41 pg/ml
Range 0.78 pg/ml - 100 pg/ml
Endothelin 1 measured in biological fluids of helathy species showing quantity (pg) per mL of tested sample. Results obtained from samples that were not extracted. 

Frataxin ELISA Kit - ab115346 from Abcam

Category ELISA Kit
Sensitivity 0.3 ng/ml
Range 107 %Sample specific recoverySample typeAverage %RangeCell culture supernatant10786% - 118%Serum10799% - 111%
Example standard curve.

Haptoglobin Bovine ELISA Kit - ab137977 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
Sensitivity > 3.7 ng/ml
Range 3.75 ng/ml - 240 ng/ml
Representative Standard Curve using ab137977

IFN gamma Cow ELISA Kit - ab190515 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
Sensitivity 0.021 ng/ml
Range 0.031 ng/ml - 2 ng/ml
Representative standard curve using ab190515.

IgA Cow ELISA Kit - ab190516 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
Sensitivity 7.009 ng/ml
Range 12.5 ng/ml - 400 ng/ml
Representative standard curve using ab190516.

IgG Cow ELISA Kit - ab190517 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
Sensitivity 1.398 ng/ml
Range 7.813 ng/ml - 500 ng/ml
Representative standard curve using ab190517

IgM Cow ELISA Kit - ab190518 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
Sensitivity 3.006 ng/ml
Range 12.5 ng/ml - 400 ng/ml
Representative standard curve using ab190518 IgM Cow ELISA Kit

IL-17 (Interleukin-17) Pig ELISA Kit - ab193732 from Abcam

Category ELISA Kit
Prices $713.00
Sizes 1 x 96 tests
Sensitivity 14 pg/ml
Range 14 pg/ml - 4000 pg/ml

IL-2 (Interleukin-2) Bovine ELISA Kit - ab193682 from Abcam

Category ELISA Kit
Prices $713.00
Sizes 1 x 96 tests
Sensitivity 0.2 ng/ml
Range 0.2 ng/ml - 200 ng/ml

Insulin Human ELISA Kit - ab100578 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
Sensitivity < 4 µlU/ml
Range 4.69 µlU/ml - 300 µlU/ml
Human Insulin measured in cell culture supernatants showing quantity (µIU) per mL of tested sample.

Interferon-gamma (IFNG) Bovine ELISA Kit - ab193681 from Abcam

Category ELISA Kit
Prices $475.00
Sizes 1 x 96 tests
Sensitivity 0.11 ng/ml
Range 0.11 ng/ml - 30 ng/ml
Example of typical bovine IFN gamma standard curve using Assay Diluent (used for serum/plasma standards).

MitoBiogenesis™ In-Cell ELISA Kit (Colorimetric) - ab110217 from Abcam

Category ELISA Kit
Prices $464.00
Sizes 2 x 96 tests
Inhibition of mitochondrial biogenesis by chloramphenicol The IC50 of a drug's effect on mitochondrial protein translation can be determined quickly using the MitoBiogenesis™ In-Cell ELISA Kit. In this example, cells were seeded at 6000 cells/well, allowed to grow for 3 cell doublings in a drug dilution series and then the relative amounts of COX-I, and SDH-A were measured in each well. Chloramphenicol inhibits mtDNA-encoded COX-I protein synthesis relative to nuclear DNA-encoded SDH-A protein synthesis by 50% at 8.1 µM.

MitoBiogenesis™ In-Cell ELISA Kit (Fluorescent) - ab140359 from Abcam

Category ELISA Kit
Prices $438.00
Sizes 1 x 96 tests
The IC50 of a drug’s effect on mitochondrial protein translation can be determined quickly using the MitoBiogenesis™ ICE Kit. In this example, cells were seeded at 6,000 cells/well, allowed to grow for 6 days in a drug dilution series and then relative amounts of COX-I (HRP signal), and SDH-A (AP-signal) were measured in each well. Chloramphenicol inhibits mtDNA-encoded (HRP signal) COX-I protein synthesis relative to nuclear DNA-encoded (AP signal) SDH-A protein synthesis by 50% at 8.1 µM, %CV = 4.33% for HRP (COX-1) signal and 3.13% for AP (SDH-A) signal.

MitoBiogenesis™ In-Cell ELISA Kit (IR) - ab110216 from Abcam

Category ELISA Kit
Prices $464.00
Sizes 2 x 96 tests
Inhibition of mitochondrial biogenesis by chloramphenicol The IC50 of a drug's effect on mitochondrial protein translation can be determined quickly using the MitoBiogenesis™ In-Cell ELISA Kit (IR). In this example, cells were seeded at 3000 cells/well, allowed to grow for 3 cell doublings in a drug dilution series and then the relative amounts of COX-I, and SDH-A were measured in each well. Chloramphenicol inhibits mtDNA-encoded COX-I protein synthesis relative to nuclear DNA-encoded SDH-A protein synthesis by 50% at 3.5 µM.

NADH Dehydrogenase (Complex I) Profiling ELISA Kit - ab124535 from Abcam

Category ELISA Kit
Sensitivity 20 µg/ml
Range 3 µg/ml - 1000 µg/ml
Example control sample curve of serially titrated HepG2 cell extracts in the working range of the assay.

PDH E1 alpha protein (PDHA1) ELISA Kit - ab115342 from Abcam

Category ELISA Kit
Sensitivity = 15 ng/ml
Example of standard curve constructed using ab115342.

Phospho S232 PDH E1 alpha protein (PDHA1) Profiling ELISA Kit - ab115343 from Abcam

Category ELISA Kit
Prices $448.00
Sizes 1 x 96 tests
Sensitivity = 31 µg/ml
Range 31 µg/ml - 1000 µg/ml
The PDHA1 bound from undosed HeLa cells was subject to in-well kinase treatment (PDK1&3) or in-well phosphatase treatment (PDP1) according to the supplementary protocol shown below. Untreated cells showed a significant endogenous phosphorylation signal at S232 which is only slightly increased by kinase treatment. Conversely phosphatase treatment was able to significantly reduce the phospho S232 signal from the endogenous levels.

Phospho S293 PDH E1 alpha protein (PDHA1) Profiling ELISA Kit - ab115344 from Abcam

Category ELISA Kit
Sensitivity = 63 µg/ml
Range 63 µg/ml - 1000 µg/ml
Example control sample curve. The phosphorylation state of PDHA1 can vary by treatment but also by cell culture conditions such as media supplements, nutrients and also cell density.
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