ATP2A2

Gene Symbol ATP2A2
Entrez Gene 488
Alt Symbol ATP2B, DAR, DD, SERCA2
Species Human
Gene Type protein-coding
Description ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
Other Description ATPase, Ca++ dependent, slow-twitch, cardiac muscle-2|SR Ca(2+)-ATPase 2|calcium pump 2|calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform|cardiac Ca2+ ATPase|endoplasmic reticulum class 1/2 Ca(2+) ATPase|sarcoplasmic/endoplasmic reticulum calcium ATPase 2
Swissprots P16615 Q86VJ2 P16614 A6NDN7 B4DF05
Accessions AAA52757 AAA52758 ABY71268 ACA51040 EAW97902 EAW97903 EAW97904 EAW97905 EAW97906 EAW97907 P16615 AK000300 AK095382 AK293877 BAG57266 AL117505 AY186578 AAO47398 BC035588 AAH35588 BC065753 BG773489 BI553880 BX648282 M23114 AAA53193 M23115 AAA53194 XM_005253888 XP_005253945 XM_011538402 XP_011536704 XM_011538403 XP_011536705 XR_243009 NM_001681 NP_001672 NM_170665 NP_733765 NM_001135765
Function This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Isoform 2 is involved in the regulation of the contraction/relaxation cycle. {ECO:0000269|PubMed:16402920}.
Subcellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein. Sarcoplasmic reticulum membrane; Multi-pass membrane protein.
Tissue Specificity Isoform 1 is widely expressed in smooth muscle and nonmuscle tissues such as in adult skin epidermis, with highest expression in liver, pancreas and lung, and intermediate expression in brain, kidney and placenta. Also expressed at lower levels in heart and skeletal muscle. Isoforms 2 and 3 are highly expressed in the heart and slow twitch skeletal muscle. Expression of isoform 3 is predominantly restricted to cardiomyocytes and in close proximity to the sarcolemma. Both isoforms are mildly expressed in lung, kidney, liver, pancreas and placenta. Expression of isoform 3 is amplified during monocytic differentiation and also observed in the fetal heart. {ECO:0000269|PubMed:10441324, ECO:0000269|PubMed:12659872, ECO:0000269|PubMed:16402920}.
Top Pathways cAMP signaling pathway, Alzheimer's disease, cGMP-PKG signaling pathway, Adrenergic signaling in cardiomyocytes, Pancreatic secretion

ATP2A2/SERCA2 (D51B11) Rabbit mAb - 9580 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D51B11
Western blot analysis of extracts from various cell types using ATP2A2/SERCA2 (D51B11) Rabbit mAb.

ATP2A2/SERCA2 Antibody - 4388 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell types using ATP2A2/SERCA2 Antibody.

SERCA2 (F-1) - sc-376235 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SERCA2 (F-1): sc-376235. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

SERCA2 (C-20) - sc-8094 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
SERCA2 (C-20): sc-8094. Western blot analysis of SERCA2 expression in rat heart extract.

SERCA2 (IID8) - sc-53010 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SERCA2 (IID8): sc-53010. Western blot analysis of SERCA2 expression in MDCK whole cell lysate.

SERCA2 (N-19) - sc-8095 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
SERCA2 (N-19) : sc-8095. Western blot analysis of SERCA2 expression in rat cardiac tissue extract.

SERCA2 (4i351) - sc-73022 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SERCA2 (4i351): sc-73022. Western blot analysis of SERCA2 expression in MDCK whole cell lysate.

Anti-SERCA2 ATPase antibody - ab130733 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-SERCA2 ATPase antibody (ab130733) at 1/100 dilution + K562 cell line lysate at 35 µg

Anti-SERCA2 ATPase antibody [EPR9392] - ab150435 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9392
Overlay histogram showing HepG2 cells stained with ab150435 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab150435, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-SERCA2 ATPase antibody [EPR9392] (Alexa Fluor® 488) - ab195544 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9392
Overlay histogram showing HeLa cells stained with ab195544 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab195544, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-SERCA2 ATPase antibody [EPR9392] (Alexa Fluor® 647) - ab195546 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9392
ab195546 staining SERCA2 ATPase in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab195546 at a 1/50 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-SERCA2 ATPase antibody [EPR9393] - ab137020 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9393
Overlay histogram showing HepG2 cells stained with ab137020 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab137020, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-ATP2A2 / SERCA2 Antibody (aa373-403, AP) - LS-C251974 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ATP2A2 / SERCA2 Antibody (aa373-403, APC) - LS-C251975 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ATP2A2 / SERCA2 Antibody (aa373-403, FITC) - LS-C251977 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ATP2A2 / SERCA2 Antibody (aa373-403, Biotin) - LS-C251976 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ATP2A2 / SERCA2 Antibody (aa373-403, Azide-free, HRP) - LS-C251978 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ATP2A2 / SERCA2 Antibody - LS-C192370 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of ATP2A2 pAb in extracts from mouse heart tissue.

Anti-ATP2A2 / SERCA2 Antibody (aa373-403) - LS-C156649 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
ATP2A2 Antibody western blot of K562 cell line lysates (35 ug/lane). The ATP2A2 antibody detected the ATP2A2 protein (arrow).

Anti-ATP2A2 / SERCA2 Antibody (aa373-403, PE) - LS-C251979 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ATP2A2 / SERCA2 Antibody (aa373-403) - LS-C319686 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ATP2A2 / SERCA2 Antibody - LS-C341038 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-ATP2A2 / SERCA2 Antibody (aa475-525) - LS-C287299 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Pig, Rat and Rabbit SERCA2 by Western Blot and Immunoprecipitation. Sample: Homogenate from pig (lanes 1 and 2) or rabbit (lane 4) aorta or lysate from cultured rat aortic smooth muscle cells (lane 3). Antibody: Affinity purified rabbit anti-SERCA2 used at 1 ug/ml (lanes 2 and 4) or 0.4 ug/ml (lane 3) for WB and 2 ug/mg lysate for IP or control (control) monoclonal anti-SERCA2 (lanes 1 and 4) used at 1 ug/ml for WB. Detection: Chemiluminescence.

Anti-ATP2A2 / SERCA2 Antibody - LS-C331256 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using ATP2A2 antibody.

Anti-ATP2A2 / SERCA2 Antibody (aa665-679) - LS-C312872 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ATP2A2 / SERCA2 antibody. IHC(P): Human Breast Cancer Tissue.
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