NOTCH1

Gene Symbol NOTCH1
Entrez Gene 4851
Alt Symbol AOS5, AOVD1, TAN1, hN1
Species Human
Gene Type protein-coding
Description notch 1
Other Description Notch homolog 1, translocation-associated|neurogenic locus notch homolog protein 1|translocation-associated notch protein TAN-1
Swissprots P46531 Q59ED8 Q5SXM3
Accessions AFA51166 AFA51167 AFD02281 AFD64628 AHH85782 AHJ25884 CBX47648 CCQ43558 EAW88241 P46531 AB209873 BAD93110 AF308602 AAG33848 AK000012 BAA90883 BC013208 BC039147 BC046127 BC049843 BC063597 CN431067 CR457221 CAG33502 DA324222 M73980 AAA60614 XM_011518717 XP_011517019 NM_017617 NP_060087
Function Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved
Subcellular Location Cell membrane {ECO:0000250}; Single-pass type I membrane protein {ECO:0000250}.
Tissue Specificity In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.
Top Pathways Notch signaling pathway, Prion diseases, MicroRNAs in cancer, Dorso-ventral axis formation

Cleaved Notch1 (Val1744) Antibody - 2421 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from 293 cells transfected with full-length Notch1, either untreated or treated with 5 mM EDTA for 15 minutes, and MOLT-4 extracts, using Cleaved Notch 1 (Val1744) Antibody.

Cleaved Notch1 (Val1744) (D3B8) Rabbit mAb - 4147 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D3B8
Western blot analysis of extracts from various cell lines using Cleaved Notch1 (Val1744) (D3B8) Rabbit mAb (upper) or Notch1 (D1E11) XP ® Rabbit mAb #3608 (lower).

Notch1 (D6F11) XP ® Rabbit mAb - 4380 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D6F11
Western blot analysis of extracts from HPB-ALL, and Molt4 using Notch1 (D6F11) XP ® Rabbit Ab. The full-length (FL) and transmembrane/intracellular region (NTM) are indicated.

Notch1 (D1E11) XP ® Rabbit mAb - 3608 from Cell Signaling Technology

Prices $277.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D1E11
Western blot analysis of total cell extract from various cell types using Notch1 (D1E11) XP ® Rabbit mAb. The full-length (FL) Notch protein and the cleaved transmembrane/intracellular region (NTM) are indicated.

Notch1 (5B5) Rat mAb - 3447 from Cell Signaling Technology

Prices $256.00
Sizes 100 µl (10 western blots)
Host Rat
Clonality Monoclonal
Clone 5B5
Western blot analysis of extracts from A2780 and A204 cells using Notch1 (5B5) Rat mAb. The transmembrane/intracellular region (NTM) is indicated.

Notch1 (C37C7) Rabbit mAb - 3439 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C37C7
Western blot analysis of extracts from various cell lines using Notch1 (C37C7) Rabbit mAb.

Notch1 (C44H11) Rabbit mAb - 3268 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C44H11
Western blot analysis of extracts from various cell lines using Notch1 (C44H11) Rabbit mAb. The transmembrane/intracellular region (NTM) is indicated.

Notch1 (6A5) Rabbit mAb - 2495 from Cell Signaling Technology

Host Rabbit
Clonality Monoclonal
Clone 6A5
Western blot analysis of extracts from COS-7 cells, transfected with Notch1 (lane 1), mock transfected (lane 2) or 3T3-L1 cells differentiated for 10 days (lane 3), using Notch1 (6A5) Rabbit mAb (left) and Cleaved Notch 1 (Val1744) Antibody (2421) (right).

Notch 1 (C-10) - sc-373891 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Notch 1 (C-10): sc-373891. Western blot analysis of Notch 1 expression in non-transfected: sc-117752 (A) and truncated mouse Notch 1 transfected: sc-110326 (B) 293T whole cell lysates.

Notch 1 (A-8) - sc-376403 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Notch 1 (A-8): sc-376403. Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing cytoplasmic staining of glandular cells.

Notch 1 (C-20) - sc-6014 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Notch 1 (C-20): sc-6014. Western blot analysis of Notch 1 expression revealing full length and truncated transmembrane forms in FHs 173We (A) and H4 (B) whole cell lysates.

Notch 1 (E-4) - sc-373944 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Notch 1 (E-4): sc-373944. Western blot analysis of Notch 1 expression in non-transfected: sc-117752 (A) and truncated mouse Notch 1 transfected: sc-110326 (B) 293T whole cell lysates.

Notch 1 (G-20) - sc-23301 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Notch 1 (G-20): sc-23301. Western blot analysis of Notch 1 expression in non-transfected: sc-117752 (A) and truncated mouse Notch 1 transfected: sc-110326 (B) whole cell lysates

Notch 1 (H-131) - sc-9170 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Notch 1 (H-131): sc-9170. Immunofluorescence staining of normal mouse intestine frozen section showing membrane staining.

Notch 1 (L-18) - sc-23299 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Notch 1 (L-18): sc-23299. Immunoperoxidase staining of formalin fixed, paraffin-embedded human lymphoma tissue showing membrane and cytoplasmic localization.

Notch 1 (mN1A) - sc-32745 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Notch 1 siRNA (h): sc-36095. Western blot analysis of Notch 1 expression in non-transfected control (A) and Notch 1 siRNA transfected (B) SK-N-MC cells. Blot probed with Notch 1 (mN1A): sc-32745. α-actinin (H-2): sc-17829 used as specificity and loading control.

Notch 1 (S-20) - sc-23304 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Notch 1 (S-20): sc-23304. Western blot analysis of Notch 1 expression in TE671 whole cell lysate.

Anti-activated Notch1 antibody - ab8925 from Abcam

Prices $400.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
ab8925 staining actived Notch1 in the NIH3T3 fibroblast cell line from Mouse embryo by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% and blocked with 10% serum for 30 minutes at 24°C. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit IgG polyclonal(1/500) was used as the secondary antibody.See Abreview

Anti-activated Notch1 antibody - ab83253 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-activated Notch1 antibody (ab83253) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Notch1 antibody - ab65297 from Abcam

Prices $398.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Notch1 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 5µg of Rabbit polyclonal to Notch1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab65297.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 120kDa; Notch1

Anti-Notch1 antibody - ab87982 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Anti-Notch1 antibody (ab87982) at 5 µg/ml + Raw 264.7 cell lysate at 50 µg

Anti-Notch1 antibody - ab93114 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-Notch1 antibody [A6] - ab44986 from Abcam

Prices $384.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone A6
ICC/IF image of ab44986 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab44986, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Notch1 antibody [bTAN 20] - ab79972 from Abcam

Prices $370.00
Sizes 100 µg
Host Rat
Clonality Monoclonal
Clone bTAN 20

Anti-Notch1 antibody [EP1238Y] (HRP) - ab194123 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1238Y
Anti-Notch1 antibody [EP1238Y] (HRP) (ab194123) at 1/5000 dilution + HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µgdeveloped using the ECL techniquePerformed under reducing conditions.
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