CLCF1

Gene Symbol	CLCF1
Entrez Gene	23529
Alt Symbol	BSF-3, BSF3, CISS2, CLC, NNT-1, NNT1, NR6
Species	Human
Gene Type	protein-coding
Description	cardiotrophin-like cytokine factor 1
Other Description	B-cell stimulating factor 3\|B-cell stimulatory factor 3\|B-cell-stimulating factor 3\|CRLF1 associated cytokine-like factor 1\|neurotrophin-1/B-cell stimulating factor-3\|novel neurotrophin-1
Swissprots	Q9UBD9 B4DNT4 Q6NZA4
Accessions	AAF00992 CAV33841 EAW74601 EAW74602 Q9UBD9 AF172854 AAD54284 AF176911 AAF00991 AK298052 BAG60346 AY049779 AAL15436 BC012939 AAH12939 BC066229 AAH66229 BC066230 AAH66230 BC066231 AAH66231 BM846622 DC393345 DQ891525 ABM82451 DQ894715 ABM85641 NM_001166212 NP_001159684 NM_013246 NP_037378
Function	Cytokine with B-cell stimulating capability. Binds to and activates the ILST/gp130 receptor. {ECO:0000269\|PubMed:10448081, ECO:0000269\|PubMed:10500198}.
Subcellular Location	Secreted {ECO:0000250}.
Tissue Specificity	Expressed predominantly in lymph nodes, spleen, peripheral blood lymphocytes, bone marrow, and fetal liver. {ECO:0000269\|PubMed:10448081, ECO:0000269\|PubMed:10500198}.
Top Pathways	Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway

NNT-1/BSF-3 (985-1) - sc-74243 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	100 µg/ml
Host	Mouse
Clonality	Monoclonal

NNT-1/BSF-3 (985-1): sc-74243. Western blot analysis of NNT-1/BSF-3 expression in non-transfected: sc-117752 (A) and human NNT-1/BSF-3 transfected: sc-175983 (B) 293T whole cell lysates.

NNT-1/BSF-3 (FL-225) - sc-33795 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

NNT-1/BSF-3 (FL-225): sc-33795. Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.

NNT-1/BSF-3 (N-14) - sc-13494 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Anti-NNT1 antibody - ab26125 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Measurement of NNT1 levels in conditioned media taken from 7 Human non-small cell lung carcinoma lines, using ab26125 as the capture antibody in a Sandwich ELISA. Conditioned media was collected from all 7 cell lines 48 hours after plating in serum-free media. A 96-well plate was coated with rabbit polyclonal to NNT1 (ab26125 at 1/500 in PBS) overnight at 4°C. 200 µL conditioned media was added to the plates and incubated overnight at 4°C. A mouse monoclonal against NNT1 was added followed by an HRP-conjugated goat anti-mouse secondary antibody. Absorbance was read at 450 nm.

Anti-NNT1 antibody - ab96733 from Abcam

Prices	$359.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-NNT1 antibody (ab96733) at 1/1000 dilutionLane 1 : H1299 whole cell lysateLane 2 : HepG2 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-NNT1 antibody - ab168295 from Abcam

Prices	$370.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

All lanes : Anti-NNT1 antibody (ab168295) at 1 µg/mlLane 1 : NNT1 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Anti-NNT1 antibody (Biotin) - ab83408 from Abcam

Prices	$370.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti-NNT1 antibody - N-terminal - ab174190 from Abcam

Prices	$370.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

Anti-NNT1 antibody - N-terminal (ab174190) at 1/100 dilution + A549 cell line lysate at 35 µg

Anti-CLCF1 Antibody (aa45-72, AP) - LS-C213324 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-CLCF1 Antibody (aa45-72, APC) - LS-C213325 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-CLCF1 Antibody (aa45-72, PE) - LS-C213328 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-CLCF1 Antibody (aa45-72, FITC) - LS-C213326 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-CLCF1 Antibody (aa45-72, Azide-free, HRP) - LS-C213327 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-CLCF1 Antibody (aa28-225, Biotin) - LS-C297929 from LifeSpan Bioscience

Prices	$435.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-CLCF1 Antibody (aa88-190, Biotin) - LS-C298396 from LifeSpan Bioscience

Prices	$435.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-CLCF1 Antibody (aa88-190, FITC) - LS-C305089 from LifeSpan Bioscience

Prices	$455.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-CLCF1 Antibody (aa28-225, FITC) - LS-C304843 from LifeSpan Bioscience

Prices	$465.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-CLCF1 Antibody (aa45-72) - LS-C320416 from LifeSpan Bioscience

Prices	$345.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-CLCF1 Antibody - LS-C306967 from LifeSpan Bioscience

Prices	$455.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

Anti-CLCF1 Antibody IHC-plus™ - LS-B8220 from LifeSpan Bioscience

Prices	$395.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti-CLCF1 antibody IHC of human lung, macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8220 dilution 5 ug/ml.

Anti-CLCF1 Antibody (aa88-190) - LS-C293180 from LifeSpan Bioscience

Prices	$405.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-CLCF1 Antibody (aa28-225) - LS-C293182 from LifeSpan Bioscience

Prices	$415.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-CLCF1 Antibody (aa45-72) - LS-C159360 from LifeSpan Bioscience

Prices	$295.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

CLCF1 Antibody western blot of A549 cell line lysates (35 ug/lane). The CLCF1 antibody detected the CLCF1 protein (arrow).

Anti-CLCF1 Antibody (aa1-225) - LS-C186046 from LifeSpan Bioscience

Prices	$335.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunofluorescence of methanol-fixed HeLa using NNT1 antibody at 1:200 dilution.

Anti-CLCF1 Antibody (Biotin) - LS-C104491 from LifeSpan Bioscience

Prices	$285.00, $325.00
Sizes	25 µg, 50 µg
Host	Rabbit
Clonality	Polyclonal

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