FLT3

Gene Symbol FLT3
Entrez Gene 2322
Alt Symbol CD135, FLK-2, FLK2, STK1
Species Human
Gene Type protein-coding
Description fms-related tyrosine kinase 3
Other Description CD135 antigen|FL cytokine receptor|fetal liver kinase 2|fms-like tyrosine kinase 3|growth factor receptor tyrosine kinase type III|receptor-type tyrosine-protein kinase FLT3|stem cell tyrosine kinase 1
Swissprots F5H0A0 B7ZLT7 P36888 Q13414 A0AVG9 B7ZLT8
Accessions AAB52916 CEO43471 EAX08424 EAX08425 P36888 AW057705 AW772610 BC036028 BC126350 AAI26351 BC144039 AAI44040 BC144040 AAI44041 BI461248 L36162 AAA35487 U02687 AAA18947 Z26652 CAA81393 XM_011535015 XP_011533317 XM_011535016 XP_011533318 XM_011535017 XP_011533319 XM_011535018 XP_011533320 NM_004119 NP_004110 NR_130706
Function Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. Activation of wild-type FLT3 causes only marginal activation of STAT5A or STAT5B. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways. {ECO:0000269|PubMed:10080542, ECO:0000269|PubMed:11090077, ECO:0000269|PubMed:14504097, ECO:0000269|PubMed:16266983, ECO:0000269|PubMed:16627759, ECO:0000269|PubMed:18490735, ECO:0000269|PubMed:20111072, ECO:0000269|PubMed:21067588, ECO:0000269|PubMed:2126
Subcellular Location Membrane; Single-pass type I membrane protein. Endoplasmic reticulum lumen. Note=Constitutively activated mutant forms with internal tandem duplications are less efficiently transported to the cell surface and a significant proportion is retained in an immature form in the endoplasmic reticulum lumen. The activated kinase is rapidly targeted for degradation.
Tissue Specificity Detected in bone marrow, in hematopoietic stem cells, in myeloid progenitor cells and in granulocyte/macrophage progenitor cells (at protein level). Detected in bone marrow, liver, thymus, spleen and lymph node, and at low levels in kidney and pancreas. Highly expressed in T-cell leukemia. {ECO:0000269|PubMed:18490735, ECO:0000269|PubMed:7507245, ECO:0000269|PubMed:8394751, ECO:0000269|PubMed:8637232}.
Top Pathways Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Acute myeloid leukemia, Central carbon metabolism in cancer, Transcriptional misregulation in cancer

Phospho-FLT3 (Tyr591) Antibody (Alexa Fluor® 488 Conjugate) - 3459 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

Phospho-FLT3 (Tyr591) Antibody - 3461 from Cell Signaling Technology

Prices $287.00, $678.00
Sizes 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from BaF3/FLT3 cells, untreated or stimulated with FLT3 Ligand (FL) (3 µg/ml for 5 minutes), using Phospho-FLT3 (Tyr591) Antibody (upper) or FLT3 (8F2) Rabbit mAb #3462 antibody (lower).

Phospho-FLT3 (Tyr589/591) (30D4) Rabbit mAb - 3464 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 30D4
Western blot analysis of extracts from cells expressing various activated tyrosine kinase proteins, using Phospho-FLT3 (Tyr589/591) (30D4) Rabbit mAb (upper) or Phospho-Tyrosine mAb (P-Tyr-100) #9411 (lower). Phospho-FLT3 (Tyr589/591) (30D4) Rabbit mAb specifically recognizes the activated FLT3 but not non-phosphorylated FLT3 protein.

Phospho-FLT3 (Tyr842) (10A8) Rabbit mAb - 4577 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 10A8
Western blot analysis of cell extracts from SEM cells expressing constitutively activated FLT3 using Phospho-FLT3 (Tyr842) (10A8) Rabbit mAb (upper and middle). The middle blot was treated with calf intestinal phosphatase (CIP) before antibody probing. Total FLT3 (8F2) Rabbit mAb #3462 shown at the bottom.

Phospho-FLT3 (Tyr969) (C24D9) Rabbit mAb - 3463 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C24D9
Western blot analysis of cell extracts from Baf3/FLT3 cells, untreated or treated with FLT3 ligand (FL), using Phospho-FLT3 (Tyr969) (C24D9) Rabbit mAb (upper) or FLT3 (8F2) Rabbit mAb Antibody #3462 (lower).

Phospho-FLT3 (Tyr591) (54H1) Mouse mAb - 3466 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 54H1
Western blot analysis of extracts from Baf3/FLT3 cells, untreated or stimulated with FLT-ligand (3 µg/ml for 2 minutes), using Phospho-FLT3 (Tyr591) (54H1) Mouse mAb (upper) or FLT3 antibody lower.

Phospho-FLT3 (Tyr591) (33G6) Rabbit mAb - 3474 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 33G6
Western blot analysis of SEM cell lysate untreated or treated with FLT3 inhibitor, using Phospho-FLT3 (Tyr591) Rabbit mAb (upper) and FLT3 (8F2) Rabbit mAb #3462 (lower).

FLT3 (8F2) Rabbit mAb - 3462 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 8F2
Western blot analysis of extracts from Baf3/FLT3 transfected cells and SEM leukemia cells, using FLT3 (8F2) Rabbit mAb.

Flt-3/Flk-2 (BV10) - sc-21788 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of Flt-3/Flk-2 expression in THP-1 whole cell lysate immunoprecipitated with Flt-3/Flk-2 (BV10): sc-21788 and Flt-3/Flk-2 (S-18): sc-480 as probe.

Flt-3/Flk-2 (C-20) - sc-479 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Flt-3/Flk-2 (C-20): sc-479. Western blot analysis of Flt-3/Flk-2 expression in THP-1 whole cell lysate.

Flt-3/Flk-2 (H-300) - sc-20733 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Flt-3/Flk-2 (H-300): sc-20733. Immunofluorescence staining of methanol-fixed K-562 cells showing membrane localization.

Flt-3/Flk-2 (S-18) - sc-480 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Flt-3/Flk-2 (S-18): sc-480. Western blot analysis of Flt-3/Flk-2 expression in THP-1 whole cell lysate.

Flt-3/Flk-2 (SF1.340) - sc-19635 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of Flt-3/Flk-2 immunoprecipitated from THP-1 whole cell lysate with Flt-3/Flk-2 (SF1.340): sc-19635 and detected with Flt-3/Flk-2 (C-20): sc-479.

Flt-3/Flk-2 (8H5) - sc-101343 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

Anti-Flt3 / CD135 (phospho Y589) antibody [EPR2311(2)] - ab171975 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2311(2)
All lanes : Anti-Flt3 / CD135 (phospho Y589) antibody [EPR2311(2)] (ab171975) at 1/1000 dilutionLane 1 : A431 cell lysate - untreatedLane 2 : A431 cell lysate - treated with pervanadateLysates/proteins at 10 µg per lane.

Anti-Flt3 / CD135 (phospho Y591) antibody [EPR2159(2)] - ab171953 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2159(2)
All lanes : Anti-Flt3 / CD135 (phospho Y591) antibody [EPR2159(2)] (ab171953) at 1/10000 dilutionLane 1 : Untreated A431 cell lysatesLane 2 : Pervanadate treated A431 cell lysatesLysates/proteins at 10 µg per lane.

Anti-Flt3 / CD135 (phospho Y599) antibody - ab138440 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Flt3 / CD135 (phospho Y599) antibody (ab138440) at 1/500 dilution + extracts from COS7 cells treated with 200ng/ml of EGF for 30 minutes

Anti-Flt3 / CD135 (phospho Y842) antibody - ab138447 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Flt3 / CD135 (phospho Y842) antibody (ab138447) at 1/500 dilution + HepG2 cells treated with 200 ng/ml of EGF for 30 minutes.

Anti-Flt3 / CD135 antibody - ab197838 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Flt3 / CD135 antibody (ab197838) at 1/240 dilutionLane 1 : Lovo cell lysateLane 2 : 231 cell lysateLysates/proteins at 40 µg per lane.

Anti-Flt3 / CD135 antibody - ab33085 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-Flt3 / CD135 antibody - ab131545 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescent analysis of methanol-fixed HeLa cells labelling Flt3 / CD135 with ab131545 at 1/100 dilution.

Anti-Flt3 / CD135 antibody - ab66035 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Flt3 / CD135 antibody (ab66035) at 1 µg/mlLane 1 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate (ab3957)Lane 2 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate Whole Cell Lysate Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

Anti-Flt3 / CD135 antibody - ab33092 from Abcam

Prices $372.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Flt3 / CD135 antibody [7B7C3] - ab175420 from Abcam

Host Mouse
Clonality Monoclonal
Clone 7B7C3
Anti-Flt3 / CD135 antibody [7B7C3] (ab175420) at 1/500 dilution + Flt3 / CD135 recombinant protein

Anti-Flt3 / CD135 antibody [BV10A4] - ab95141 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone BV10A4
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