FLT3

Gene Symbol FLT3
Entrez Gene 2322
Alt Symbol CD135, FLK-2, FLK2, STK1
Species Human
Gene Type protein-coding
Description fms-related tyrosine kinase 3
Other Description CD135 antigen|FL cytokine receptor|fetal liver kinase 2|fms-like tyrosine kinase 3|growth factor receptor tyrosine kinase type III|receptor-type tyrosine-protein kinase FLT3|stem cell tyrosine kinase 1
Swissprots F5H0A0 B7ZLT7 P36888 Q13414 A0AVG9 B7ZLT8
Accessions AAB52916 CEO43471 EAX08424 EAX08425 P36888 AW057705 AW772610 BC036028 BC126350 AAI26351 BC144039 AAI44040 BC144040 AAI44041 BI461248 L36162 AAA35487 U02687 AAA18947 Z26652 CAA81393 XM_011535015 XP_011533317 XM_011535016 XP_011533318 XM_011535017 XP_011533319 XM_011535018 XP_011533320 NM_004119 NP_004110 NR_130706
Function Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. Activation of wild-type FLT3 causes only marginal activation of STAT5A or STAT5B. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways. {ECO:0000269|PubMed:10080542, ECO:0000269|PubMed:11090077, ECO:0000269|PubMed:14504097, ECO:0000269|PubMed:16266983, ECO:0000269|PubMed:16627759, ECO:0000269|PubMed:18490735, ECO:0000269|PubMed:20111072, ECO:0000269|PubMed:21067588, ECO:0000269|PubMed:2126
Subcellular Location Membrane; Single-pass type I membrane protein. Endoplasmic reticulum lumen. Note=Constitutively activated mutant forms with internal tandem duplications are less efficiently transported to the cell surface and a significant proportion is retained in an immature form in the endoplasmic reticulum lumen. The activated kinase is rapidly targeted for degradation.
Tissue Specificity Detected in bone marrow, in hematopoietic stem cells, in myeloid progenitor cells and in granulocyte/macrophage progenitor cells (at protein level). Detected in bone marrow, liver, thymus, spleen and lymph node, and at low levels in kidney and pancreas. Highly expressed in T-cell leukemia. {ECO:0000269|PubMed:18490735, ECO:0000269|PubMed:7507245, ECO:0000269|PubMed:8394751, ECO:0000269|PubMed:8637232}.
Top Pathways Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Acute myeloid leukemia, Central carbon metabolism in cancer, Transcriptional misregulation in cancer

PathScan® Phospho-FLT3 (Tyr591) Chemiluminescent Sandwich ELISA Kit - 7021 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Relationship between protein concentration of lysates from phospho or nonphospho SEM cells and immediate light generation with chemiluminescent substrate is shown. SEM cells in exponential growth (10 6 cells/ml) were lysed with or without the addition of phosphatase inhibitor to the lysis buffer (phospho or nonphospho lysate). The graph inset corresponding to the shaded area shows high sensitivity and a linear response at the low protein concentration range.

PathScan ® Phospho-FLT3 (panTyr) Sandwich ELISA Kit - 7761 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1: Constitutive phosphorylated of FLT3 in SEM cells is detected by PathScan ® Phospho-FLT3 (panTyr) Sandwich ELISA kit #7761. In contrast, only a low level of phospho-FLT3 protein is detected in SEM cells treated with CT-53518, an inhibitor of FLT3 tyrosine phosphorylation. The inhibitor does not affect the level of total FLT3 protein detected by PathScan ® Total FLT3 Sandwich ELISA kit #7202. Absorbance at 450 nm is shown in the top figure, while the corresponding western blots using Phospho-FLT3 (Tyr591) Antibody #3461 (right panel) or FLT3 Rabbit mAb #3462 (left panel), is shown in the bottom figure.

PathScan ® Phospho-FLT3 (Tyr591) Sandwich ELISA Kit - 7206 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)

PathScan ® Total FLT3 Sandwich ELISA Kit - 7202 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1: Constitutive phosphorylated of FLT3 in SEM cells is detected by PathScan® Phospho-FLT3 (Tyr591) Sandwich ELISA kit #7206. In contrast, only a low level of phospho-FLT3 protein is detected in SEM cells treated with CT-53518, an inhibitor of FLT3 tyrosine phosphorylation. The inhibitor does not affect the level of total FLT3 protein detected by PathScan® Total FLT3 Sandwich ELISA kit #7202. Absorbance at 450 nm is shown in the top figure, while the corresponding western blots using Phospho-FLT3 (Tyr591) Antibody #3461 (right panel) or FLT3 Rabbit mAb #3462 (left panel), is shown in the bottom figure.

HTScan® FLT3 Kinase Assay Kit - 7743 from Cell Signaling Technology

Category Assay Kit
Figure 1. FLT3 kinase activity was measured in a radiometric assay using the following reaction conditions: 5 mM MOPS, pH 7.2, 2.5 mM β-glycerophosphate, 1 mM EGTA, 0.4 mM EDTA, 5 mM MgCl2, 0.05 mM DTT, 50 μM ATP, Substrate: MBP 200 ng/μL, and variable amounts of FLT3.

Human CD135 / FLT3 ELISA Kit (Sandwich ELISA) - LS-F4003 from LifeSpan Bioscience

Category ELISA Kit
Prices $650.00
Sizes 1 plate
Sensitivity 0.118 ng/ml
Range 0.313-20 ng/ml

Mouse/Human Phospho-CD135 / FLT3 (Tyr842) ELISA Kit (Cell-Based Phosphorylation ELISA) - LS-F1680 from LifeSpan Bioscience

Category ELISA Kit
Prices $550.00
Sizes 1 plate
Range 0.313-20 ng/ml

Human Phospho-CD135 / FLT3 (Tyr969) ELISA Kit (Cell-Based Phosphorylation ELISA) - LS-F1681 from LifeSpan Bioscience

Category ELISA Kit
Prices $550.00
Sizes 1 plate
Range 0.313-20 ng/ml

Mouse/Human CD135 / FLT3 ELISA Kit (Cell-Based ELISA) - LS-F1995 from LifeSpan Bioscience

Category ELISA Kit
Prices $450.00
Sizes 1 plate
Range 0.313-20 ng/ml

Human CD135 / FLT3 ELISA Kit (Sandwich ELISA) - LS-F11478 from LifeSpan Bioscience

Category ELISA Kit
Prices $600.00
Sizes 1 plate
Sensitivity 78
Range 0.313-20 ng/ml

Human Phospho-Flt-3/Flk-2 DuoSet IC ELISA - DYC368-2 from R&D Systems

Category ELISA Kit
Prices $329.00, $649.00, $1,395.00
Sizes 1 kit (for 2 Plates), 1 kit (for 5 Plates), 1 pack (for 15 Plates)

Human Phospho-Flt-3/Flk-2 DuoSet IC, 5 Plate - DYC368-5 from R&D Systems

Category ELISA Kit

Human Total Flt-3/Flk-2 DuoSet IC ELISA - DYC912-2 from R&D Systems

Category ELISA Kit
Prices $329.00, $649.00
Sizes 1 kit (for 2 Plates), 1 kit (for 5 Plates)
Range 156.00 - 10,000 pg/mL

Human Total Flt-3/Flk-2 DuoSet IC, 5 Plate - DYC912-5 from R&D Systems

Category ELISA Kit

Human Flt-3/Flk-2 DuoSet ELISA - DY812-05 from R&D Systems

Category ELISA Kit
Prices $299.00
Sizes 1 kit (for 5 Plates)
Range 125.00 - 8,000 pg/mL

Human FLT3 ELISA kit - 55R-2048 from Fitzgerald

Category ELISA Kit
Prices $505.00
Sizes 96 tests

Human Flt3 ELISA Kit - abx151568 from Abbexa

Category ELISA Kit
Sensitivity 0.118 ng/ml
Range 0.313 ng/ml - 20 ng/ml

FMS Like Tyrosine Kinase 3, Flt3, Human, ELISA Kit - E-EL-H0878 from ARP American Research Products

Category ELISA Kit
Prices $522.00
Sizes 96 t
Sensitivity 0.188
Range 0.313-20ng/mL

FMS-like tyrosine kinase 3, Flt3, Human, ELISA Kit - CSB-E04556h from ARP American Research Products

Category ELISA Kit
Prices $338.00, $468.00, $469.00, $471.00
Sizes 48 t, 96 t, 5 x 96t, 10 x 96t

Receptor-type tyrosine-protein kinase FLT3, FLT3, Human, ELISA Kit - E0039h from ARP American Research Products

Category ELISA Kit
Prices $476.00
Sizes 96 t
Range 78-5000 pg/mL

FMS-like Tyrosine Kinase 3, Flt3, Mouse, ELISA Kit - E-EL-M0508 from ARP American Research Products

Category ELISA Kit
Prices $585.00
Sizes 96 t

FMS Like Tyrosine Kinase 3, Flt3, Rabbit, ELISA Kit - E-EL-RB0535 from ARP American Research Products

Category ELISA Kit
Prices $585.00
Sizes 96 t

FMS Like Tyrosine Kinase 3, Flt3, Chicken, ELISA Kit - E-EL-Ch1796 from ARP American Research Products

Category ELISA Kit
Prices $522.00
Sizes 96 t
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