Gene Symbol | Uchl1 |
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Entrez Gene | 22223 |
Alt Symbol | AW822034, C88048, PGP 9.5, PGP9.5, R75593, UCH-L1, UCHL-1, gad |
Species | Mouse |
Gene Type | protein-coding |
Description | ubiquitin carboxy-terminal hydrolase L1 |
Other Description | gracile axonal dystrophy|neuron cytoplasmic protein 9.5|protein gene product 9.5|ubiquitin carboxyl-terminal hydrolase isozyme L1|ubiquitin thioesterase L1 |
Swissprots | Q9R0P9 Q9R122 |
Accessions | EDL37772 EDL37773 Q9R0P9 AB025313 BAA84083 AF172334 AAD51029 AK004066 AK013729 BAB28976 AK170728 BAE41984 BC039177 AAH39177 CX241178 JX050004 AFP20215 NM_011670 NP_035800 |
Function | Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. {ECO:0000269|PubMed:12913066}. |
Subcellular Location | Cytoplasm {ECO:0000269|PubMed:12913066}. Endoplasmic reticulum membrane {ECO:0000250}; Lipid-anchor {ECO:0000250}. |
Tissue Specificity | Expressed in brain, where it is found in neurons but not in oligodendrocytes or astrocytes. Found in the ganglion cell layer and the inner nuclear layer of the retina (at protein level). Expressed in brain and testis. In the brain, expression is at its lowest in replaceable neurons of hippocampus and olfactory bulb. Highly expressed in senescent pituitary. {ECO:0000269|PubMed:10471497, ECO:0000269|PubMed:12559414, ECO:0000269|PubMed:12913066, ECO:0000269|PubMed:14695319, ECO:0000269|PubMed:15911766}. |
UCH-L1 CRISPR/Cas9 KO Plasmid (m) - sc-423591 from Santa Cruz Biotechnology
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UCH-L1 HDR Plasmid (m) - sc-423591-HDR from Santa Cruz Biotechnology
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UCH-L1 Double Nickase Plasmid (m) - sc-423591-NIC from Santa Cruz Biotechnology
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UCH-L1 Double Nickase Plasmid (m2) - sc-423591-NIC-2 from Santa Cruz Biotechnology
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UCH-L1 CRISPR Activation Plasmid (m) - sc-423591-ACT from Santa Cruz Biotechnology
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UCH-L1 CRISPR Activation Plasmid (m2) - sc-423591-ACT-2 from Santa Cruz Biotechnology
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UCH-L1 Lentiviral Activation Particles (m) - sc-423591-LAC from Santa Cruz Biotechnology
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UCH-L1 Lentiviral Activation Particles (m2) - sc-423591-LAC-2 from Santa Cruz Biotechnology
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UCH-L1 siRNA (m) - sc-42305 from Santa Cruz Biotechnology
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UCH-L1 shRNA Plasmid (m) - sc-42305-SH from Santa Cruz Biotechnology
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UCH-L1 shRNA (m) Lentiviral Particles - sc-42305-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EMU012161 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN43872 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND43872 from Sigma-Aldrich
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UCHL1 siRNA (Mouse) - CRM4323 from Cohesion Biosciences
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UCHL1 - MBS8234989 from MyBioSource
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shRNA set against Mouse UCHL1 (NM_011670.2) - SHH439756 from Creative biogene
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Mouse PGP9.5 siRNA - orb283799 from Biorbyt
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UCHL1 shRNA Plasmid (Mouse) - CHM4323 from Cohesion Biosciences
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