ALK

Gene Symbol ALK
Entrez Gene 238
Alt Symbol CD246, NBLST3
Species Human
Gene Type protein-coding
Description anaplastic lymphoma receptor tyrosine kinase
Other Description ALK tyrosine kinase receptor|CD246 antigen|mutant anaplastic lymphoma kinase
Swissprots Q9UM73 Q4ZFX9 Q9Y4K6 Q59FI3 Q53RZ4 Q53QQ6
Accessions AAX76514 AAX88892 AAX93126 AAY15027 ACD03579 ACD03580 ACD03581 ACD03582 ACD03583 ACD03584 ACD03585 ACD03586 ACD03587 ACD03588 ACD03589 CAT16962 CCV20015 EAX00506 EAX00507 EAX00508 Q9UM73 AB209477 BAD92714 AK296218 BAG58942 BC156207 AAI56208 BC157090 AAI57091 DA455128 EU788003 ACI47591 EU788004 ACI47592 EU788005 ACI47593 EU788006 ACI47594 EU788007 ACI47595 EU788008 ACI47596 EU788009 ACI47597 EU788010 ACI47598 FJ838786 ACZ57900 FJ838787 ACZ57901 GU128152 ACY79560 GU128153 ACY79561 GU128154 ACY79562 GU128155 ACY79563 GU128156 ACY79564 GU128157 ACY79565 U62540 AAB71619 U66559 AAC51104 NM_004304 NP_004295
Function Neuronal orphan receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor s
Subcellular Location Cell membrane {ECO:0000269|PubMed:16317043, ECO:0000269|PubMed:9174053}; Single-pass type I membrane protein {ECO:0000269|PubMed:16317043, ECO:0000269|PubMed:9174053}. Note=Membrane attachment was crucial for promotion of neuron-like differentiation and cell proliferation arrest through specific activation of the MAP kinase pathway.
Tissue Specificity Expressed in brain and CNS. Also expressed in the small intestine and testis, but not in normal lymphoid cells. {ECO:0000269|PubMed:9174053}.
Top Pathways Non-small cell lung cancer

ALK (D5F3 ® ) XP ® Rabbit mAb (PE Conjugate) - 8868 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Flow cytometric analysis of DU 145 (blue) and KARPAS-299 (green) cells using ALK (D5F3 ® ) XP ® Rabbit mAb (PE Conjugate).

ALK (D5F3 ® ) XP ® Rabbit mAb (Biotinylated) - 8936 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Western blot analysis of extracts from NCI-H2228 cells using ALK (D5F3 ® ) XP ® Rabbit mAb (Biotinylated). Streptavidin-HRP #3999 was used for detection.

ALK (C26G7) Rabbit mAb (Biotinylated) - 8895 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C26G7
Western blot analysis of extracts from KARPAS-299 cells using ALK (C26G7) Rabbit mAb (Biotinylated). Streptavidin-HRP #3999 was used for detection. Cell Line Source: Dr Abraham Karpas at the University of Cambridge.

Phospho-ALK (Tyr1604) Antibody - 3341 from Cell Signaling Technology

Prices $287.00, $678.00
Sizes 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Sup-M2 cells, using Phospho-ALK (Tyr1604) Antibody (A,B) or ALK Antibody (C,D). The phospho-specificity of the antibody was characterized by treating the membrane with calf intestinal alkaline phosphatase (CIP) (B,D) after Western transfer. (Sup-M2 cells provided by Dr. Stephan W. Morris, St. Jude Children's Research Hospital, Tennessee.)

Phospho-ALK (Tyr1096) Antibody - 4143 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from KARPAS-299 cells, untreated or treated with calf intestinal phosphatase (CIP), using Phospho-ALK (Tyr1096) Antibody (upper) and ALK (C26G7) Rabbit mAb #3333 (lower). Cell Line Source: Dr Abraham Karpas at the University of Cambridge.

Phospho-ALK (Tyr1586) Antibody - 3343 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cells expressing different activated tyrosine kinases, using Phospho-ALK (Tyr1586) Antibody (upper) or Phospho-Tyrosine mAb (P-Tyr-100) #9411 (lower). Phospho-ALK (Tyr1586) Antibody shows no cross-reactivity with other tyrosine-phosphorylated tyrosine kinases.

Phospho-ALK (Tyr1078) Antibody - 4144 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from KARPAS-299 cells, untreated or treated with calf intestinal phosphatase (CIP), using Phospho-ALK (Tyr1078) Antibody (upper) and ALK (C26G7) Rabbit mAb #3333 (lower). Cell Line Source: Dr Abraham Karpas at the University of Cambridge.

Phospho-ALK (Tyr1586) (3B4) Rabbit mAb - 3348 from Cell Signaling Technology

Prices $307.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 3B4
Western blot analysis of Sup-M2 cell lysate, using Phospho-ALK (Tyr1586) (3B4) Rabbit mAb (A and B), and ALK Antibody (C and D).The phospho-specificity of this antibody was characterized by treating the membrane with calf intestinal alkaline phosphatase (CIP) (B and D) after Western transfer.

Phospho-ALK (Tyr1282/1283) Antibody - 3714 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Sup-M2 and Karpas-299 cells using Phospho-ALK (Tyr1282/1283) Antibody (upper and middle) and ALK (C26G7) Rabbit mAb #3333 (lower). The middle and lower blots were treated with calf intestinal phosphatase to dephosphorylate NPM-ALK. Cell Line Source: Dr Abraham Karpas at the University of Cambridge.

Phospho-ALK (Tyr1096) (D96H9) Rabbit mAb - 6962 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D96H9
Western blot analysis of extracts from SUP-M2 cells, untreated or treated with calf intestinal phosphatase (CIP), using Phospho-ALK (Tyr1096) (D96H10) Rabbit mAb (upper) and ALK (C26G7) Rabbit mAb #3333 (lower).

Phospho-ALK (Tyr1278) (D59G10) Rabbit mAb - 6941 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D59G10
Western blot analysis of extracts from SUP-M2 cells, untreated or treated with calf intestinal phosphatase (CIP), using Phospho-ALK (Tyr1278) (D59G10) Rabbit mAb (upper) and ALK (C26G7) Rabbit mAb #3333 (lower).

Phospho-ALK (Tyr1078) (D28B4) Rabbit mAb - 12127 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D28B4
Western blot analysis of extracts from KARPAS-299 cells, untreated (-) or treated with calf intestinal phosphatase (CIP; +), using Phospho-ALK (Tyr1078) (D28B4) Rabbit mAb (upper) or ALK (C26G7) Rabbit mAb #3333 (lower). Cell Line Source: Dr Abraham Karpas at the University of Cambridge.

Phospho-ALK (Tyr1278/1282/1283) Antibody - 3983 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from KARPAS-299 cells, untreated or treated with calf intestinal phosphatase (CIP), using Phospho-ALK (Tyr1278/1282/1283) Antibody (upper) and ALK (C26G7) Rabbit mAb #3333 (lower). Cell Line Source: Dr Abraham Karpas at the University of Cambridge.

Phospho-ALK (Tyr1278) Antibody - 3710 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Sup-M2 and Karpas-299 cells using Phospho-ALK (Tyr1278) Antibody (upper and middle) and ALK (C26G7) Rabbit mAb #3333 (lower). The middle and lower blots were treated with calf intestinal phosphatase to dephosphorylate NPM-ALK. Cell Line Source: Dr Abraham Karpas at the University of Cambridge.

Phospho-ALK (Tyr1282/1283) (D39B2) Rabbit mAb - 9687 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D39B2
Western blot analysis of extracts from SUP-M2 cells, untreated or treated with calf intestinal phosphatase (CIP), using Phospho-ALK (Tyr1282/1283) (D39B2) Rabbit mAb (upper) or ALK (C26G7) Rabbit mAb #3333 (lower). The lower molecular weight cluster is partial degradation of the ALK fusion.

ALK (D5F3 ® ) XP ® Rabbit mAb - 3633 from Cell Signaling Technology

Prices $169.00, $502.00
Sizes 20 µl (2 western blots), 100 µl (250 slides)
Host Rabbit
Clonality Monoclonal
Western blot analysis of extracts from NCI-H2228 and NCI-H3122 cells using ALK (D5F3 ® ) XP ® Rabbit mAb. Variants denoting fusions of different EML4 exons (v1 or v3) are indicated.

ALK (31F12) Mouse mAb - 3791 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 31F12
Western blot analysis of extracts from Sup-M2 and KARPAS-299 cells using ALK (31F12) Mouse mAb. Cell Line Source: Dr Abraham Karpas at the University of Cambridge.

ALK (C26G7) Rabbit mAb - 3333 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C26G7
Western blot analysis of extracts from Karpas-299 and Sup-M2 cells using ALK (C26G7) Rabbit mAb. Cell Line Source: Dr Abraham Karpas at the University of Cambridge.

ALK Antibody - 3342 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

ALK (ALK1) - sc-53157 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

ALK (F-12) - sc-398791 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ALK (F-12): sc-398791. Western blot analysis of ALK expression in HuT 78 (A), U-937 (B), Raji (C), K-562 (D), MDA-MB-231 (E) and MDA-MB-435S (F) whole cell lysates.

ALK (C-19) - sc-6344 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ALK (C-19): sc-6344. Western blot analysis of ALK expression in K-562 whole cell lysate.

ALK (H-260) - sc-25447 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

ALK (N-19) - sc-6346 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

ALK (T-18) - sc-6345 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
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