GTF2F1

Gene Symbol GTF2F1
Entrez Gene 2962
Alt Symbol BTF4, RAP74, TF2F1, TFIIF
Species Human
Gene Type protein-coding
Description general transcription factor IIF, polypeptide 1, 74kDa
Other Description TFIIF-alpha|general transcription factor IIF 74 kDa subunit|general transcription factor IIF subunit 1|transcription initiation factor IIF subunit alpha|transcription initiation factor RAP74
Swissprots B2RCS0 Q9BWN0 P35269
Accessions EAW69098 EAW69099 P35269 AA323384 AK293124 BAG56676 AK302178 BAG63544 AK315240 BAG37667 BC000120 AAH00120 BC013007 AAH13007 BT007097 AAP35761 CR456798 CAG33079 DQ890597 ABM92214 DQ893766 ABM84692 X64002 CAA45404 X64037 CAA45408 NM_002096 NP_002087
Function TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. {ECO:0000269|PubMed:10428810}.
Subcellular Location Nucleus.
Top Pathways Basal transcription factors

TFIIF-α Antibody - 8080 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using TFIIF-α Antibody.

TFIIF RAP 74 (C-18) - sc-235 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
TFIIF RAP 74 (C-18): sc-235. Western blot analysis of TFIIF RAP 74 expression in NIH/3T3 nuclear extract.

TFIIF RAP 74 (N-16) - sc-234 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
TFIIF RAP 74 (N-16): sc-234. Western blot analysis of TFIIF RAP 74 expression in non-transfected 293T: sc-117752 (A), mouse TFIIF RAP 74 transfected 293T: sc-124005 (B) and NIH/3T3 (C) whole cell lysates.

Anti-GTF2F1 antibody - ab192479 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-GTF2F1 antibody - ab94651 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-GTF2F1 antibody (ab94651) at 1 µg/ml (in 5% skim milk / PBS buffer) + HT1080 cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-GTF2F1 antibody - ab130487 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-GTF2F1 antibody (ab130487) at 1/100 dilution + T47D cell line lysates at 35 µg

Anti-TFIIF antibody - ab15862 from Abcam

Prices $406.00
Sizes 50 µg
Host Chicken
Clonality Polyclonal

Anti-TFIIF antibody [2A15] - ab4449 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2A15

Anti-TFIIF antibody - ChIP Grade - ab28179 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10mins. The  ChIP was performed with 25µg of chromatin, 8µg of ab28179 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes for the actively transcribed genes GAPDH and ACT1 are located in the core promoter.

Anti-TFIIF antibody - ChIP Grade - ab28180 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10mins. The  ChIP was performed with 25µg of chromatin, 8µg of  ab28180 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes for the actively transcribed genes GAPDH and ACT1 are located in the core promoter.

Anti-RAP74 / GTF2F1 Antibody (aa231-258) - LS-C18141 from LifeSpan Bioscience

Prices $475.00
Sizes 150 µg
Host Mouse
Clonality Monoclonal

Anti-RAP74 / GTF2F1 Antibody (aa213-258) - LS-C18142 from LifeSpan Bioscience

Prices $475.00
Sizes 150 µg
Host Mouse
Clonality Monoclonal

Anti-RAP74 / GTF2F1 Antibody (aa409-452) - LS-C18143 from LifeSpan Bioscience

Prices $475.00
Sizes 150 µg
Host Mouse
Clonality Monoclonal

Anti-RAP74 / GTF2F1 Antibody (aa411-435) - LS-C18145 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal

Anti-RAP74 / GTF2F1 Antibody (C-Terminus) - LS-C190154 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RAP74 / GTF2F1 Antibody (aa178-206, AP) - LS-C258144 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RAP74 / GTF2F1 Antibody (aa178-206, APC) - LS-C258145 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RAP74 / GTF2F1 Antibody (aa178-206, FITC) - LS-C258146 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RAP74 / GTF2F1 Antibody (aa178-206, Biotin) - LS-C258147 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RAP74 / GTF2F1 Antibody (aa178-206, Azide-free, HRP) - LS-C258148 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RAP74 / GTF2F1 Antibody (aa178-206, PE) - LS-C258149 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RAP74 / GTF2F1 Antibody (aa350-400) - LS-C289468 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RAP74 / GTF2F1 Antibody (aa177-206) - LS-C321823 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RAP74 / GTF2F1 Antibody - LS-C341743 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RAP74 / GTF2F1 Antibody (N-Terminus) - LS-C30151 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
A: Marker, B: K562 Cell Lysate
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