GRIN2B

Gene Symbol	GRIN2B
Entrez Gene	2904
Alt Symbol	EIEE27, GluN2B, MRD6, NMDAR2B, NR2B, hNR3
Species	Human
Gene Type	protein-coding
Description	glutamate receptor, ionotropic, N-methyl D-aspartate 2B
Other Description	N-methyl D-aspartate receptor subtype 2B\|N-methyl-D-aspartate receptor subunit 3\|NR3\|glutamate [NMDA] receptor subunit epsilon-2\|glutamate receptor ionotropic, NMDA 2B\|glutamate receptor subunit epsilon-2
Swissprots	Q12919 Q14CU4 Q13225 Q9UM56 Q13224 Q13220
Accessions	EAW96311 Q13224 AB208850 BAD92087 AK091524 BC113618 AAI13619 BC113620 AAI13621 BX648060 U11287 AAB60368 U28758 AAA74930 U28861 AAA69919 U28862 AAA69920 U88963 AAD00659 U90278 AAB49993 XM_005253351 XP_005253408 XM_011520628 XP_011518930 XM_011520629 XP_011518931 XM_011520630 XP_011518932 NM_000834 NP_000825
Function	NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death (By similarity). {ECO:0000250}.
Subcellular Location	Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
Tissue Specificity	Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia. {ECO:0000269\|PubMed:9547169}.
Top Pathways	Rap1 signaling pathway, Circadian entrainment, Alcoholism, Cocaine addiction, cAMP signaling pathway

Phospho-NMDAR2B (Tyr1472) Antibody - 4208 from Cell Signaling Technology

Prices	$287.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of extracts from rat brain, either 15 min ischemia followed by 4 h reperfusion or 15 min ischemia only, using Phospho-NMDAR2B (Tyr1472) Antibody (upper) or NMDAR2B (4207, lower).

Phospho-NMDAR2B (Tyr1070) Antibody - 4209 from Cell Signaling Technology

Prices	$287.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of extracts from rat brain, either sham-operated, 15 min ischemia followed by 4 h reperfusion or 15 min ischemia only, using Phospho-NMDAR2B (Tyr1070) Antibody (upper) or NMDAR2B #4207 Antibody (lower).

Phospho-NMDAR2B (Ser1284) Antibody - 5355 from Cell Signaling Technology

Prices	$287.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of extracts from rat brain using Phospho-NMDAR2B (Ser1284) Antibody. The phospho-specificity of the antibody was verified by blocking with a phospho or non-phosphopeptide.

NMDAR2B Antibody - 4207 from Cell Signaling Technology

Prices	$246.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of extracts from mouse and rat brain using NMDAR2B Antibody.

NMDAR2B (D8E10) Rabbit mAb - 14544 from Cell Signaling Technology

Prices	$246.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Clone	D8E10

Western blot analysis of extracts from mouse and rat brain using NMDAR2B (D8E10) Rabbit mAb.

NMDAR2B (D15B3) Rabbit mAb - 4212 from Cell Signaling Technology

Prices	$246.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Clone	D15B3

Western blot analysis of extracts from mouse and neonatal mouse brain using NMDAR2B (D15B3) Rabbit mAb.

NMDAR2B (N59/36.1) Mouse mAb - 5580 from Cell Signaling Technology

Host	Mouse
Clonality	Monoclonal
Clone	N59/36.1

Western blot analysis of extracts from fetal rat brain and rat brain using NMDAR2B (N59/36.1) Mouse mAb.

NMDAε2 (C-20) - sc-1469 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

NMDAε2 (C-20): sc-1469. Western blot analysis of glutamate receptor (NMDAε2/NR2B) expression in mouse brain tissue extract.

NMDAε2 (H-20) - sc-31545 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

NMDAε2 (H-50) - sc-9057 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

NMDAε2 (H-50): sc-9057. Western blot analysis of human recombinant NMDAε2 fusion protein.

NMDAε2 (N-20) - sc-31543 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

p-NMDAε2 (Tyr 1336) - sc-135703 from Santa Cruz Biotechnology

Prices	$295.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

p-NMDAε2 (Tyr 1336): sc-135703. Western blot analysis of NMDAε2 phosphorylation in untreated (A) and lambda protein phosphatase-treated (B) rat hippocampal tissue extracts.

Anti-NMDAR2B (phospho S1303) antibody - ab138459 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-NMDAR2B (phospho S1303) antibody (ab138459) at 1/500 dilution + Mouse brain extract at 30 µg

Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] - ab81271 from Abcam

Prices	$357.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EP1858Y

All lanes : Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271) at 1/5000 dilutionLane 1 : mouse brain lysates (untreated)Lane 2 : mouse brain lysates (treated with AP)Lysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP labelled IgG at 1/2000 dilution

Anti-NMDAR2B (phospho Y1070) antibody [EPR13436] - ab181102 from Abcam

Prices	$357.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR13436

All lanes : Anti-NMDAR2B (phospho Y1070) antibody [EPR13436] (ab181102) at 1/2000 dilutionLane 1 : Mouse cortex lysateLane 2 : Mouse cortex lysate treated with alkaline phosphataseLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-NMDAR2B (phospho Y1252) antibody - ab18532 from Abcam

Prices	$380.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Lanes 1 - 3 : Anti-NMDAR2B (phospho Y1252) antibody (ab18532) at 3 µg/mlLane 4 : NR2B antibody (positive control)Lane 1 : mouse cortical extract with Mouse NMDAR2B (unmodified ) peptide (ab19637) at 2 µg/mlLane 2 : mouse cortical extract with Mouse NMDAR2B (phospho Y1252) peptide (ab19636) at 2 µg/mlLane 3 : mouse cortical extractLane 4 : mouse cortical extractLysates/proteins at 20 µg per lane.

Anti-NMDAR2B (phospho Y1336) antibody - ab193286 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-NMDAR2B (phospho Y1336) antibody (ab193286) at 1/500 dilutionLane 1 : Jurkat cell extract treated with TNFLane 2 : Jurkat cell extract treated with TNF with treated with antigen-specific peptide

Anti-NMDAR2B (phospho Y1336) antibody - ab138664 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-NMDAR2B (phospho Y1336) antibody (ab138664) at 1/500 dilution + Jurkat cell extracts, treated with TNF (20 ng/ml, 30 mins) at 30 µg

Anti-NMDAR2B (phospho Y1336) antibody - ab37173 from Abcam

Prices	$370.00
Sizes	50 µl
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-NMDAR2B (phospho Y1336) antibody (ab37173) at 1/1000 dilutionLane 1 : rat hippocampal lysate Lane 2 : rat hippocampal lysate with incubated in lambda Ptase (1200 units for 30 min) before being exposed to ab37173Lysates/proteins at 30 µg per lane.

Anti-NMDAR2B (phospho Y1474) antibody - ab194923 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-NMDAR2B (phospho Y1474) antibody (ab194923) at 1/500 dilutionLane 1 : Untreated A431 cell extractsLane 2 : EGF-treated A431 cell extracts

Anti-NMDAR2B antibody - ab14400 from Abcam

Prices	$376.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti-NMDAR2B antibody - ab79302 from Abcam

Prices	$376.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-NMDAR2B antibody (ab79302) at 1/500 dilutionLane 1 : Extracts from A549 cellsLane 2 : Extracts from A549 cells with immunising peptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-NMDAR2B antibody [13A11] - ab78508 from Abcam

Prices	$359.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	13A11

Anti-NMDAR2B antibody [NR2B] - ab28373 from Abcam

Prices	$384.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	NR2B

Anti-NMDAR2B antibody [NR2B] (ab28373) at 2 µg/ml + mouse brain tissue

Anti-NMDAR2B antibody [S59-36] - ab93610 from Abcam

Prices	$381.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	S59-36

Overlay histogram showing SH-SY5Y cells stained with ab93610 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93610, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed SH-SY5Y cells used under the same conditions.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback

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