DOK1

Gene Symbol DOK1
Entrez Gene 1796
Alt Symbol P62DOK
Species Human
Gene Type protein-coding
Description docking protein 1, 62kDa (downstream of tyrosine kinase 1)
Other Description Downstream of tyrosine kinase 1|docking protein 1|docking protein 1 (downstream of tyrosine kinase 1)|p62(dok)|pp62
Swissprots Q53TY2 O43204 Q99704 Q9UHG6
Accessions AAX93224 EAW99612 EAW99613 EAW99614 Q99704 AF035299 AAB88182 AF180527 AAF19167 AK055944 BAG51595 AK127127 BAG54440 AK127656 BAG54544 AK222883 BAD96603 AK296154 BAG58893 AK300097 BAG61898 AK314678 BC111055 AAI11056 BC114440 AAI14441 BC114497 AAI14498 BQ575925 DA382785 DA599419 U70987 AAC51127 XM_011532669 XP_011530971 XM_011532670 XP_011530972 NM_001197260 NP_001184189 NM_001381 NP_001372
Function DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3. {ECO:0000269|PubMed:18156175}.
Subcellular Location Isoform 1: Cytoplasm. Nucleus.
Tissue Specificity Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells. Expressed in breast cancer. {ECO:0000269|PubMed:12595900}.
Top Pathways Measles

p62Dok Antibody - 3912 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from CHO cells transfected with wild-type (WT) or mutant (Y362F) p62Dok, using p62Dok Antibody.

Dok-1 (C-19) - sc-6374 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Dok-1 (C-19): sc-6374. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

Dok-1 (E-16) - sc-46093 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Dok-1 (E-16): sc-46093. Western blot analysis of Dok-1 expression in Jurkat whole cell lysate.

Dok-1 (G-18) - sc-46094 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Dok-1 (G-18): sc-46094. Immunoperoxidase staining of formalin fixed, paraffin-embedded human tonsil tissue showing cytoplasmic staining of squamous epithelial cells.

Dok-1 (45) - sc-135888 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
Dok-1 (45): sc-135888. Western blot analysis of Dok-1 expression in Hs68 whole cell lysate.

Dok-1 (M-19) - sc-6277 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Dok-1 (M-19): sc-6277. Western blot analysis of Dok-1 expression in CTLL-2 whole cell lysate.

Dok-1 (L-15) - sc-46095 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Dok-1 (M-276) - sc-6934 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of Dok-1 p62 expression in CTLL-2 (A), Jurkat (B) and HeLa (C) whole cell lysates. Antibodies tested include Dok-1 (A-3): sc-6929 (A) and Dok-1 (M-276): sc-6934 (B-C).

Dok-1 (P-19) - sc-46096 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Dok-1 (P-19): sc-46096. Western blot analysis of Dok-1 expression in Jurkat (A), K-562 (B) and HEL 92.1.7 (C) whole cell lysates.

Dok-1 (A-3) - sc-6929 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Dok-1 (A-3): sc-6929. Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of exocrine pancreas and islet cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

p-Dok-1 (Tyr 362) - sc-101766 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of phosphorylated Dok-1 expression in Jurkat whole cell lysates (A, B). Blots were probed with p-Dok-1 (Tyr 362): sc-101766: sc-101761 preincubated with its cognate phosphorylated peptide (A) and p-Dok-1 (Tyr 362): sc-101766 (B).

p-Dok-1 (Tyr 398) - sc-101767 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-Dok-1 (Tyr 398): sc-101767. Western blot analysis of phosphorylated Dok-1 expression in K-562 (A) and serum starved K-562 (B) whole cell lysates.

Anti-DOK1 (phospho S450) antibody - ab76172 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DOK1 (phospho S450) antibody (ab76172) at 1/1000 dilutionLane 1 : Jurkat cells stimulated with calyculin A (100 nM, 30 min)Lane 2 : Jurkat cells stimulated with calyculin A (100 nM, 30 min) followed by lambda phosphatase.

Anti-DOK1 (phospho Y362) antibody - ab75742 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab75742, at a 1/50 dilution, staining DOK1 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunizing phospho peptide.

Anti-DOK1 (phospho Y362) antibody - ab47374 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DOK1 (phospho Y362) antibody (ab47374) at 1/500 dilutionLane 1 : Extracts from JK cells. P-peptide (-)Lane 2 : Extracts from JK cells. P-peptide (+)

Anti-DOK1 (phospho Y362) antibody - ab76173 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DOK1 (phospho Y362) antibody (ab76173) at 1/1000 dilutionLane 1 : Jurkat cells stimulated with calyculin A (100 nM, 30 min) Lane 2 : Jurkat cells stimulated with calyculin A (100 nM, 30 min), treated with alkaline phosphatase

Anti-DOK1 (phospho Y398) antibody - ab75741 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab75741 at 1/50 dilution staining DOK1 in human breast carcinoma by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising phosphopeptide.

Anti-DOK1 (phospho Y398) antibody - ab47375 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DOK1 (phospho Y398) antibody (ab47375) at 1/500 dilutionLane 1 : Extracts from K562 cells, treated with StarvationLane 2 : Untreated extracts from K562 cells

Anti-DOK1 antibody - ab8112 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DOK1 antibody (ab8112) at 1 µg/mlLane 1 : Jurkat cell lysatesLane 2 : THP-1 cell lysateObserved band size : 62 kDa (why is the actual band size different from the predicted?)

Anti-DOK1 antibody - ab37835 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-DOK1 antibody (ab37835) at 1/100 dilution + HL-60 cell lysate.SecondaryHRP-anti-rabbit

Anti-DOK1 antibody - ab47505 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DOK1 antibody (ab47505)Lane 1 : extracts from JK cells,Lane 2 : extracts from JK cells, with immunizing peptide

Anti-DOK1 antibody - ab47506 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DOK1 antibody (ab47506) at 1/500 dilutionLane 1 : K562 cell extract, untreated.Lane 2 : K562 cell extract, treated with immunising peptide (1ug/ml).Lysates/proteins at 30 µg per lane.SecondaryAlkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) at 0.12 µg/ml

Anti-DOK1 antibody - ab126806 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DOK1 antibody (ab126806) at 1/1000 dilutionLane 1 : 3T6 cell lysate at 3 µgLane 2 : 3T6 cell lysate at 10 µgLane 3 : 3T6 cell lysate at 30 µg

Anti-DOK1 antibody [EPR3228(2)] - ab155947 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3228(2)
All lanes : Anti-DOK1 antibody [EPR3228(2)] (ab155947) at 1/1000 dilutionLane 1 : HuT-78 cell lysateLane 2 : K562 cell lysateLane 3 : Jurkat cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-DOK1 antibody, prediluted - ab53207 from Abcam

Prices $370.00
Sizes 7 ml
Host Rabbit
Clonality Polyclonal
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