LIMK1

Gene Symbol LIMK1
Entrez Gene 3984
Alt Symbol LIMK, LIMK-1
Species Human
Gene Type protein-coding
Description LIM domain kinase 1
Other Description LIM motif-containing protein kinase
Swissprots Q15821 B7Z6I8 Q75MU3 Q15820 D3DXF5 O15283 P53667 D3DXF4 Q9Y5Q1
Accessions AAB17545 AAB17546 AAC13885 AAC13886 AAS07436 AAS07437 AAS07438 EAW69620 EAW69621 EAW69622 EAW69623 P53667 AB209557 BAD92794 AF134379 AAD25742 AK026544 AK125156 AK290162 BAF82851 AK300382 BAH13274 AW016257 BC148340 AAI48341 BC152982 AAI52983 D26309 BAA05371 DC296332 NM_001204426 NP_001191355 NM_002314 NP_002305 NM_016735
Function Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop. LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin- 2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton. In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation. Phosphorylates TPPP on serine residues, thereby promoting microtubule disassembly. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. Required for atypical chemokine receptor ACKR2-induced phospho
Subcellular Location Cytoplasm. Nucleus. Note=Predominantly found in the cytoplasm.
Tissue Specificity Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.
Top Pathways Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton

Phospho-LIMK1 (Thr508)/LIMK2 (Thr505) Antibody - 3841 from Cell Signaling Technology

Prices $109.00, $287.00, $678.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from COS cells, untransfected (lane 1), transfected with Wild-type LIMK1 (lanes 2 and 3) or with LIMK1 T508A mutant (lanes 4 and 5), using Phospho-LIMK1 (Thr508)/LIMK2 (Thr505) Antibody (top), LIMK1 Antibody #3842 (middle) or HA-Tag (262K) mAb #2362 (bottom). Cells were either untreated (lanes 1, 2 and 4) or treated with PMA (lanes 3 and 5). (Triple HA-tagged LIMK1 plasmids kindly provided by Dr. K. Mizuno, Biological Institute, Tohoku University, Japan.)

LIMK1 Antibody - 3842 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HEK293, A431, NIH/3T3, COS and PC12 cells, using LIMK1 Antibody.

LIMK-1 (C-10) - sc-28370 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
LIMK-1 (C-10): sc-28370. Western blot analysis of LIMK-1 expression in HeLa (A), A-431 (B), NIH/3T3 (C) and PC-12 (D) whole cell lysates.

LIMK-1 (C-18) - sc-8387 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
LIMK-1 (C-18): sc-8387. Western blot analysis of LIMK-1 expression in NIH/3T3 whole cell lysate.

LIMK-1 (E-7) - sc-48346 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
LIMK-1 (E-7): sc-48346. Western blot analysis of LIMK-1 expression in PC-12 (A), HeLa (B) and NIH/3T3 (C) whole cell lysates.

LIMK-1 (G-17) - sc-30696 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
LIMK-1 (G-17): sc-30696. Western blot analysis of LIMK-1 expression in Hep G2 whole cell lysate.

LIMK-1 (H-84) - sc-5576 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
LIMK-1 (H-84): sc-5576. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining.

LIMK-1 (L-16) - sc-30698 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

LIMK-1 (42) - sc-135973 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
LIMK-1 (42): sc-135973. Western blot analysis of LIMK-1 expression in rat cerebellum tissue extract.

Anti-LIM kinase 1 + 2 antibody - ab51200 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab51200 LIMK1/2 antibody.

Anti-LIM Kinase 1 (phospho T508) antibody - ab38508 from Abcam

Prices $390.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab38508 at a 1:50 dilution staining LIM Kinase in Human breast carcinoma tissue using Immunohistochemistry, Paraffin Embedded Tissue. Left image : untreated. Right image : treated with phosphopeptide.

Anti-LIM Kinase 1 antibody - ab39641 from Abcam

Prices $379.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-LIM Kinase 1 antibody (ab39641) at 1/500 dilutionLane 1 : NIH/3T3 cell extractsLane 2 : A2780 cell extracts

Anti-LIM Kinase 1 antibody - ab87971 from Abcam

Prices $376.00
Sizes 200 µl
Host Mouse
Clonality Monoclonal
Anti-LIM Kinase 1 antibody (ab87971) at 1/500 dilution + recombinant protein fragment (the immunogen) at 0.1 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-LIM Kinase 1 antibody - ab64819 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-LIM Kinase 1 antibody (ab64819) at 1/500 dilutionLane 1 : mouse brain cell extractLane 2 : mouse brain cell extract with immunising peptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-LIM Kinase 1 antibody - ab55414 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
LIM Kinase 1 antibody (ab55414) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human stomach.

Anti-LIM Kinase 1 antibody - ab10561 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-LIM Kinase 1 antibody [1A8] - ab117623 from Abcam

Prices $385.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 1A8
sELISA showing the detection limit for recombinant proprietary tagged LIM Kinase 1 is approximately 0.03ng/ml as a capture antibody (ab117623).

Anti-LIM Kinase 1 antibody [3G3] - ab119084 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3G3
All lanes : Anti-LIM Kinase 1 antibody [3G3] (ab119084) at 1/2000 dilutionLane 1 : HEK293T cell lysate transfected the pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected the pCMV6-ENTRY LIM Kinase 1 Lysates/proteins at 5 µg per lane.

Anti-LIM Kinase 1 antibody [EPR912] - ab108507 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR912
All lanes : Anti-LIM Kinase 1 antibody [EPR912] (ab108507) at 1/1000 dilutionLane 1 : U-87 MG cell lysateLane 2 : SH SY5Y cell lysateLane 3 : Ramos cell lysateLysates/proteins at 10 µg per lane.

Anti-LIM kinase 2 (phospho T505) + LIMK1 (phospho T508) antibody - ab131341 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence analysis of methanol-fixed HeLa cells using ab131341 at a 1/100 dilution.

Anti-LIM kinase 2 (phospho T505) + LIMK1 (phospho T508) antibody - ab131343 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescent analysis of methanol-fixed HeLa cells labelling LIM kinase 2 with ab131343 at 1/100 dilution.

Anti-LIMK1 (phospho T508) antibody - ab194798 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling LIMK1 (phospho T508) using ab194798 at 1/50 dilution.

Anti-LIMK1 antibody - ab81046 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-LIMK1 antibody (ab81046) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Rat Cortex Tissue Lysate Lane 3 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate Lane 4 : SK N BE (Human neuroblastoma) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-LIMK1 antibody - ab95186 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-LIMK1 antibody (ab95186) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgdeveloped using the ECL technique

Anti-LIMK1 antibody - ab95183 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Detection of Human LIMK1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: ab95183 was used for IP at 3 ug/mg lysate (lane 1). LIMK1 was also immunoprecipitated by another antibody (lane 2) and control IgG (lane 3). Detection: Chemiluminescence with an exposure time of 5 seconds.
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