GSK3B

Gene Symbol GSK3B
Entrez Gene 2932
Alt Symbol -
Species Human
Gene Type protein-coding
Description glycogen synthase kinase 3 beta
Other Description GSK-3 beta|GSK3beta isoform|glycogen synthase kinase-3 beta|serine/threonine-protein kinase GSK3B
Swissprots Q9UL47 D3DN89 Q9BWH3 P49841
Accessions AAC69340 AAD48517 CBX51856 CBX51857 CBX51858 CBX51859 EAW79533 EAW79534 EAW79535 EAW79536 P49841 AB451356 BAG70170 AK290897 BAF83586 AY123976 AAM88578 BC000251 AAH00251 BC012760 AAH12760 BX640779 CA312249 CR536510 CAG38748 DA477189 DQ892501 ABM83427 DQ895714 ABM86640 EU302497 ABX89591 L33801 AAA66475 XM_006713610 XP_006713673 XM_006713611 XP_006713674 NM_001146156 NP_001139628 NM_002093 NP_002084
Function Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. Requires primed phosphorylation of the majority of its substrates. In skeletal muscle, contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. May also mediate the development of insulin resistance by regulating activation of transcription factors. Regulates protein synthesis by controlling the activity of initiation factor 2B (EIF2BE/EIF2B5) in the same manner as glycogen synthase. In Wnt signaling, GSK3B forms a multimeric complex with APC, AXIN1 and CTNNB1/beta-catenin and phosphorylates the N-terminus of CTNNB1 leading to its degradation mediated by ubiquiti
Subcellular Location Cytoplasm. Nucleus. Cell membrane. Note=The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosphorylated form to the cell membrane.
Tissue Specificity Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. Colocalizes with EIF2AK2/PKR and TAU in the Alzheimer disease (AD) brain. {ECO:0000269|PubMed:21029237}.
Top Pathways Insulin signaling pathway, Cell cycle, Basal cell carcinoma, PI3K-Akt signaling pathway, B cell receptor signaling pathway

Phospho-GSK-3β (Ser9) (D85E12) XP ® Rabbit mAb (Alexa Fluor ® 647 Conjugate) - 14332 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D85E12
Flow cytometric analysis of PC-3 cells, untreated (green) or treated with LY294002 #9901 and Wortmannin #9951 (blue), using Phospho-GSK-3β (Ser9) (D85E12) XP ® Rabbit mAb (Alexa Fluor ® 647 Conjugate).

Phospho-GSK-3β (Ser9) (D85E12) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate) - 14026 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D85E12
Flow cytometric analysis of PC-3 cells, untreated (green) or treated with LY294002 #9901 and Wortmannin #9951 (blue), using Phospho-GSK-3β (Ser9) (D85E12) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate).

Phospho-GSK-3β (Ser9) (D85E12) XP ® Rabbit mAb (Pacific Blue ™ Conjugate) - 14310 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D85E12
Flow cytometric analysis of PC-3 cells, untreated (green) or treated with LY294002 #9901 and Wortmannin #9951 (blue), using Phospho-GSK-3β (Ser9) (D85E12) XP ® Rabbit mAb (Pacific Blue ™ Conjugate).

Phospho-GSK-3β (Ser9) (D85E12) XP ® Rabbit mAb (PE Conjugate) - 8466 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D85E12
Flow cytometric analysis of NIH/3T3 cells treated with hPDGF-BB #8912 and λ phosphatase (red), untreated (blue), or treated with hPDGF-BB #8912 only (green) using Phospho-GSK-3β (Ser9) (D85E12) XP ® Rabbit mAb (PE Conjugate).

GSK-3β (27C10) Rabbit mAb (Alexa Fluor ® 488 Conjugate) - 9317 from Cell Signaling Technology

Host Rabbit
Clonality Monoclonal
Clone 27C10

Phospho-GSK-3β (Ser9) (D85E12) XP ® Rabbit mAb - 5558 from Cell Signaling Technology

Prices $119.00, $318.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D85E12
Western blot analysis of extracts from GSK-3α (-/-) (lanes 1,2) and GSK-3β (-/-) (lanes 3,4) mouse embryonic fibroblast (MEF) cells, λ phosphatase or PDGF-treated, using Phospho-GSK-3β (Ser9) (D85E12) XP ® Rabbit mAb (upper) and α/β-Tubulin Antibody #2148 (lower). (MEF wild type, GSK-3α (-/-) and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).

Phospho-GSK-3-beta (Ser9) (D3A4) Rabbit mAb - 9322 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D3A4
Western blot analysis of extracts from NIH/3T3 cells, lambda-phosphatase or PDGF-treated, using Phospho-GSK-3-beta (Ser9) (D3A4) Rabbit mAb (upper) or GSK-3-beta (27C10) Rabbit mAb #9315 (lower).

Phospho-GSK-3β (Ser9) Antibody - 9336 from Cell Signaling Technology

Prices $287.00, $678.00
Sizes 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from NIH/3T3 cells, λ phosphatase treated or untreated, using Phospho-GSK-3bβ (Ser9) Antibody (upper) or GSK-3β (27C10) Rabbit mAb #9315 (lower).

Phospho-GSK-3β (Thr390) Antibody - 3548 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa cells, either hydroxyurea-treated (4 mM, 20 hrs) to induce G1/S phase arrest or paclitaxel-treated (100 nM/ml, 20 hrs) for G2/M phase arrest, using Phospho-GSK-3β (Thr390) Antibody (upper) or GSK-3β (27C10) Rabbit mAb #9315 (lower) to show induction of phospho-GSK and equal loading.

Phospho-GSK-3β (Ser9) (D2Y9Y) Mouse mAb - 14630 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone D2Y9Y
Western blot analysis of extracts from PC-3 cells, untreated (-) or treated with LY294002 #9901 (50 μM, overnight; +) and Wortmannin #9951 (1 μM, overnight; +), using Phospho-GSK-3β (Ser9) (D2Y9Y) Mouse mAb (upper) and GSK-3β (3D10) Mouse mAb #9832 (lower).

Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb - 9323 from Cell Signaling Technology

Prices $109.00, $307.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 5B3
Western blot analysis of extracts from NIH/3T3 cells, λ-phosphatase- or PDGF-treated, using Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb (upper) or GSK-3β (27C10) Rabbit mAb #9315 (lower).

GSK-3β (D5C5Z) XP ® Rabbit mAb - 12456 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D5C5Z
Western blot analysis of extracts from wild-type, GSK-3α (-/-), and GSK3β (-/-) mouse embryonic fibroblasts (MEFs) using GSK-3β (D5C5Z) XP ® Rabbit mAb (upper) and GSK-3α/β (D75D3) XP ® Rabbit mAb #5676 (lower). (MEF wild type, GSK-3α (-/-), and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).

GSK-3β (27C10) Rabbit mAb - 9315 from Cell Signaling Technology

Prices $256.00, $637.00
Sizes 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Monoclonal
Clone 27C10
Western blot analysis of extracts from HeLa, NIH/3T3, COS, C6 and 293 cells using GSK-3β (27C10) Rabbit mAb.

GSK-3β (3D10) Mouse mAb - 9832 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 3D10
Western blot analysis of extracts from wild-type, GSK-3α (-/-), and GSK3β (-/-) mouse embryonic fibroblasts (MEFs) and HeLa cells using GSK-3β (3D10) Mouse mAb (upper) and GSK-3α/β (D75D3) XP ® Rabbit mAb #5676 (lower). (MEF wild type, GSK-3α (-/-) and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).

GSK-3beta Antibody - 9332 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

GSK-3β (L-17) - sc-8257 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GSK-3β (L-17): sc-8257. Western blot analysis of authentic rabbit GSK-3β.

GSK-3β (H-76) - sc-9166 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
GSK-3β (H-76): sc-9166. Western blot analysis of GSK-3β expression in A-431 (A), PDGF-treated A-431 (B), 3611-RF (C) and PDGF-treated 3611-RF (D) whole cell lysates.

GSK-3β (11B9) - sc-81462 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
GSK-3β (11B9): sc-81462. Western blot analysis of GSK-3β expression in non-transfected 293T: sc-117752 (A), mouse GSK-3β transfected 293T: sc-120654 (B) and Jurkat (C) whole cell lysates.

GSK-3β (2Q274) - sc-71188 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
GSK-3β (2Q274): sc-71188. Western blot analysis of GSK-3β expression in HeLa (A), A549 (B), SK-BR-3 (C) and Jurkat (D) whole cell lysates.

GSK-3β (1F7) - sc-53931 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
GSK-3β (1F7): sc-53931. Western blot analysis of GSK-3β expression in HeLa (A), A549 (B), SK-BR-3 (C) and Jurkat (D) whole cell lysates.

GSK-3β (1V001) - sc-71186 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
GSK-3β (1V001): sc-71186. Western blot analysis of GSK-3β expression in HeLa (A), A549 (B), SK-BR-3 (C) and Jurkat (D) whole cell lysates.

p-GSK-3β (Ser 9) - sc-11757 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-GSK-3β (Ser 9): sc-11757. Western blot analysis of phosphorylated GSK-3β expression in untreated (A) and PDGF-treated NIH/3T3 (B) whole cell lysates.

p-GSK-3α/β (Tyr 279/Tyr 216) - sc-11758 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-GSK-3α/β (Tyr 279/Try 216)-R: sc-11758-R. Western blot analysis of phosphorylated GSK-3α and phosphorylated GSK-3β expression in A-431 (A) and PDGF-treated A-431 (B) whole cell lysates.

p-GSK-3β (2D3) - sc-81494 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
p-GSK-3β (2D3): sc-81494. Western blot analysis of GSK-3β phosphorylation in non-transfected: sc-117752 (A), untreated mouse GSK-3β transfected: sc-120654 (B) and lambda protein phosphatase treated mouse GSK-3β transfected: sc-120654 (C) 293T whole cell lysates.

p-GSK-3β (Ser 21) - sc-130601 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-GSK-3β (Ser 21): sc-130601. Western blot analysis of GSK-3β phosphorylation in A-431 whole cell lysate.
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