HDAC4

Gene Symbol HDAC4
Entrez Gene 9759
Alt Symbol AHO3, BDMR, HA6116, HD4, HDAC-4, HDAC-A, HDACA
Species Human
Gene Type protein-coding
Description histone deacetylase 4
Other Description histone deacetylase A
Swissprots Q86YH7 Q9UND6 F5GX36 P56524 E9PGB9
Accessions AAX93070 AAX93254 EAW71165 EAW71166 P56524 AB006626 BAA22957 AF070607 AF132607 AAD29046 AI199675 AI654062 AK302719 BAH13789 AK303368 BAH13951 AK303467 BAH13968 AK303617 BAH13999 AK308791 AK310488 AK310603 AK311582 BC039904 AAH39904 BQ576173 XM_006712877 XP_006712940 XM_006712878 XP_006712941 XM_006712879 XP_006712942 XM_006712880 XP_006712943 XM_011512217 XP_011510519 XM_011512218 XP_011510520 XM_011512219 XP_011510521 XM_011512220 XP_011510522 XM_011512221 XP_011510523 XM_011512222 XP_011510524 XM_011512223 XP_011510525 XM_011512224 XP_011510526 XM_011512225 XP_011510527 XM_011512226 XP_011510528 XM_011512227 XP_011510529 XM_011512228 XP_011510530 XM_011512229 XP_011510531 XM_011512230 XP_011510532 NM_006037 NP_006028
Function Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. {ECO:0000269|PubMed:10523670, ECO:0000269|PubMed:24413532}.
Subcellular Location Nucleus. Cytoplasm. Note=Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4 and SIK1. The nuclear localization probably depends on sumoylation.
Tissue Specificity Ubiquitous.
Top Pathways Viral carcinogenesis, Epstein-Barr virus infection, Alcoholism, MicroRNAs in cancer

Phospho-HDAC4 (Ser632)/HDAC5 (Ser661)/HDAC7 (Ser486) Antibody - 3424 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from DO11.10 thymocyte hybridoma cells, untreated (-) or treated with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (0.2 µM, 1 hr; +) and Ionomycin, Calcium Salt #9995 (0.33 µM, 1 hr; +), using Phospho-HDAC4 (Ser632)/HDAC5 (Ser661)/HDAC7 (Ser486) Antibody (upper). Phospho-specificity of the antibody was determined by treating cell extracts with λ phosphatase. Total HDAC proteins were detected using Histone Deacetylase 4 (HDAC4) Antibody #2072, Histone Deacetylase 5 (HDAC5) Antibody #2082 and Histone Deacetylase 7 (HDAC7) Antibody #2882.

Phospho-HDAC4 (Ser246)/HDAC5 (Ser259)/HDAC7 (Ser155) (D27B5) Rabbit mAb - 3443 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D27B5
Western blot analysis of NIH/3T3 cells using Phospho-HDAC4 (Ser246)/HDAC5 (Ser259)/HDAC7 (Ser155) (D27B5)

HDAC4 (4A3) Mouse mAb - 5392 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 4A3
Western blot analysis of extracts from various cell lines using HDAC4 (4A3) Mouse mAb.

Histone Deacetylase 4 (HDAC4) Antibody - 2072 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell types or tissue using Histone Deacetylase 4 (HDAC4) Antibody.

HDAC4 (D15C3) Rabbit mAb - 7628 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (20 western blots)
Host Rabbit
Clonality Monoclonal
Clone D15C3
Western blot analysis of extracts from various cell lines using HDAC4 (D15C3) Rabbit mAb.

HDAC4 (B-5) - sc-365093 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
HDAC4 (A-4): sc-365093. Western blot analysis of HDAC4 expression in THP-1 (A) and J774.A1 (B) whole cell lysates.

HDAC4 (A-4) - sc-46672 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
HDAC4 (A-4): sc-46672. Immunoperoxidase staining of formalin fixed, paraffin-embedded human small intestine tissue showing nuclear and cytoplasmic staining of glandular cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

HDAC4 (F-6) - sc-365367 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
HDAC4 (F-6): sc-365367. Western blot analysis of HDAC4 expression in MOLT-4 whole cell lysate.

HDAC4 (D-1) - sc-48390 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
HDAC4 (D-1): sc-48390. Western blot analysis of HDAC4 expression in HeLa (A), Jurkat (B) and NIH/3T3 (C) whole cell lysates and HeLa (D) and Jurkat (E) nuclear extracts.

HDAC4 (H-92) - sc-11418 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
HDAC4 (H-92): sc-11418. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.

HDAC4 (L-19) - sc-5246 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
HDAC4 (L-19): sc-5246. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.

HDAC4 (N-18) - sc-5245 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
HDAC4 (N-18): sc-5245. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic and nuclear localization.

HDAC4 (HDAC-144) - sc-56686 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
HDAC4 (HDAC-144): sc-56686. Western blot analysis of HDAC4 expression in non-transfected: sc-117752 (A) and human HDAC4 transfected: sc-115502 (B) 293T whole cell lysates.

p-HDAC4 (Ser 632) - sc-101691 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-HDAC4 (Ser 632): sc-101691. Western blot analysis of phosphorylated HDAC4 expression in untreated (A) and Calculin A-treated (B) Jurkat whole cell lysates.

Anti-HDAC4 (phospho S632) antibody - ab39408 from Abcam

Prices $391.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-HDAC4 (phospho S632) antibody (ab39408) at 1/500 dilutionLane 1 : Extract from Jurkat cells (5-30ug of total protein)Lane 2 : Extract from Jurkat cells treated with Calyculin A (5-30ug of total protein)

Anti-HDAC4 antibody - ab12172 from Abcam

Prices $394.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-HDAC4 antibody (ab12172) at 1/500 dilutionLane 1 : Lysate from wt control mouse heart tissueLane 2 : Lysate from Gq TG mouse heart tissueLysates/proteins at 20 µg per lane.SecondaryHRP conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilutiondeveloped using the ECL technique

Anti-HDAC4 antibody - ab111318 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab111318 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab111318 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-HDAC4 antibody - ab12170 from Abcam

Prices $372.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-HDAC4 antibody - ab16339 from Abcam

Prices $384.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry was performed on normal biopsies of deparaffinized Human skin tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a Rabbit Polyclonal Antibody recognizing HDAC4 (ab16339 ) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Anti-HDAC4 antibody - ab76884 from Abcam

Host Mouse
Clonality Monoclonal
Anti-HDAC4 antibody (ab76884) at 5 µg/ml + immunogen (recombinant tagged fragment) at 0.2 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

Anti-HDAC4 antibody - ab11968 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
HDAC4 detection by Western blot. The analysis of HDAC4 in 293 cell extract with anti-HDAC4 pAb. A protein band of approximate molecular weight of 140 kDa was detected.

Anti-HDAC4 antibody - ab117269 from Abcam

Prices $378.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
ab117269, at 1/50 dilution, staining HDAC4 in Formalin-fixed, Paraffin-embedded Human Skin tissue by Immunohistochemistry, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-HDAC4 antibody - ab123513 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Detection of HDAC4 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab123513 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 3 seconds.

Anti-HDAC4 antibody - ab39657 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-HDAC4 antibody (ab39657) at 1/500 dilutionLane 1 : Jurkat cell extractsLane 2 : Jurkat cell extracts with blocking peptide at 1 µg/ml

Anti-HDAC4 antibody [HDAC-144] - ChIP Grade - ab12171 from Abcam

Prices $388.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone HDAC-144
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