ELAVL1

Gene Symbol ELAVL1
Entrez Gene 1994
Alt Symbol ELAV1, HUR, Hua, MelG
Species Human
Gene Type protein-coding
Description ELAV like RNA binding protein 1
Other Description ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R)|ELAV-like protein 1|Hu antigen R|embryonic lethal, abnormal vision, drosophila, homolog-like 1|hu-antigen R
Swissprots Q9BTT1 Q53XN6 B4DVB8 Q15717
Accessions CCA78939 EAW68949 EAW68950 Q15717 AI375368 AK022334 AK096659 AK301013 BAG62630 AL713686 AW139417 BC003376 AAH03376 BM664469 BM696191 BT009793 AAP88795 BU542524 BX647856 EU176230 ABW03681 EU176535 ABW03336 U38175 AAB41913 W37464 XM_011527777 XP_011526079 NM_001419 NP_001410
Function RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability. Involved in embryonic stem cells (ESCs) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESCs differentiation. Binds to poly-U elements and AU- rich elements (AREs) in the 3'-UTR of target mRNAs. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro. With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA. {ECO:0000269|PubMed:19029303}.
Subcellular Location Cytoplasm. Nucleus. Note=Translocates into the cytoplasm following phosphorylation by MAPKAPK2.
Tissue Specificity Ubiquitous.
Top Pathways AMPK signaling pathway

ELAVL1/HuR (D9W7E) Rabbit mAb - 12582 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D9W7E
Western blot analysis of extracts from various cell lines using ELAVL1/HuR (D9W7E) Rabbit mAb.

HuR (G-5) - sc-374285 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
HuR (G-5): sc-374285. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.

HuR (G-8) - sc-365816 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
HuR (G-8): sc-365816. Western blot analysis of HuR expression in HeLa whole cell lysate.

HuR (H-280) - sc-20694 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
HuR (H-280): sc-20694. Immunoperoxidase staining of formalin fixed, paraffin-embedded human parathyroid gland tissue showing nuclear staining of glandular cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

HuR (N-16) - sc-5483 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
HuR (N-16): sc-5483. Western blot analysis of HuR expression in Jurkat whole cell lysate.

Anti-HuR / ELAVL1 antibody - ab196626 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunofluorscence analysis of MCF7 cells labeling HuR / ELAVL1 with ab196626  at 1/100 dilution (top panel). Blue: DAPI for nuclear staining (bottom panel). 

Anti-HuR / ELAVL1 antibody - ab135740 from Abcam

Prices $384.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-HuR / ELAVL1 antibody (ab135740) at 1/100 dilutionLane 1 : HeLa cell line lysateLane 2 : Jurkat cell line lysateLane 3 : CEM cell line lysateLane 4 : Ramos cell line lysateLysates/proteins at 35 µg per lane.

Anti-HuR / ELAVL1 antibody - ab54987 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
ab54987 Immunoprecipitating HuR / ELAVL1 in Human lymphocyte cell lysate. 700µg of cell lysate was incubated with primary antibody (5 µg/mg in abcam non-denaturing buffer) and matrix (Protein G) for 2 hours at 4°C. For western blot, ab54987 (1/1000) was used to confirm successful immunoprecipitation. Lane 1 - 25μg of lysate pre-IP, Lanes 2 and 3 -15μg of 70μg elution from matrix. 4-12% Bis-Tris gel.See Abreview

Anti-HuR / ELAVL1 antibody [14F11] - ab118057 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 14F11
All lanes : Anti-HuR / ELAVL1 antibody [14F11] (ab118057) at 1/100 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY HuR / ELAVL1 cDNALysates/proteins at 5 µg per lane.

Anti-HuR / ELAVL1 antibody [19F12AE12] - ab170193 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 19F12AE12
All lanes : Anti-HuR / ELAVL1 antibody [19F12AE12] (ab170193) at 1 µg/mlLane 1 : Human Liver HomogenateLane 2 : Human Heart HomogenateLane 3 : HepG2 Cell LysateLane 4 : HeLa Cell LysateLane 5 : SH-SY5Y Cell LysateLane 6 : Jurkat Cell LysateLysates/proteins at 20 µg per lane.SecondaryGoat anti-mouse IR800 at 1/5000 dilutiondeveloped using the ECL technique

Anti-HuR / ELAVL1 antibody [4C8] - ChIP Grade - ab136542 from Abcam

Prices $379.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4C8
Overlay histogram showing MCF7 cells stained with ab136542 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab136542, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-HuR / ELAVL1 antibody [4G8] - ab110081 from Abcam

Prices $387.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 4G8
Overlay histogram showing MCF7 cells stained with ab110081 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab110081, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was an anti-mouse Alexa Fluor® 488 (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-HuR / ELAVL1 antibody [EPR17397] - ab200342 from Abcam

Prices $347.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR17397
All lanes : Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342) at 1/5000 dilutionLane 1 : 293 (Human embryonic kidney) whole cell lysateLane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysateLane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysateLane 4 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-ELAVL1 / HUR Antibody (PE) - LS-C278291 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ELAVL1 / HUR Antibody - LS-C310240 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ELAVL1 / HUR Antibody - LS-C310241 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-ELAVL1 / HUR Antibody - LS-C314093 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-ELAVL1 / HUR Antibody (aa1-101, Azide-free) - LS-C327591 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-ELAVL1 / HUR Antibody (clone 224CT6.5.3) - LS-C319148 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 224CT6.5.3

Anti-ELAVL1 / HUR Antibody - LS-C167718 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Mouse
Clonality Monoclonal
Confocal immunofluorescent of ELAVL1 Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).

Anti-ELAVL1 / HUR Antibody - LS-C327593 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ELAVL1 / HUR Antibody (aa1-13) IHC-plus™ - LS-B2227 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-HuR antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2227 concentration 5 ug/ml.

Anti-ELAVL1 / HUR Antibody (clone 14F4) - LS-C114952 from LifeSpan Bioscience

Prices $350.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 14F4
IHC of paraffin-embedded liver tissue using anti-ELAVL1 mouse monoclonal antibody. (Dilution 1:50).

Anti-ELAVL1 / HUR Antibody - LS-C331579 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry of paraffin-embedded mouse kidney using ELAVL1 antibody at dilution of 1:200 (x400 lens).

Anti-ELAVL1 / HUR Antibody (clone 4G8) IHC-plus™ - LS-B4316 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 4G8
Anti-HuR antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4316 concentration 10 ug/ml.
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