PLCG1

Gene Symbol PLCG1
Entrez Gene 5335
Alt Symbol NCKAP3, PLC-II, PLC1, PLC148, PLCgamma1
Species Human
Gene Type protein-coding
Description phospholipase C, gamma 1
Other Description 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate|1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1|1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1|1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1|PLC-148|PLC-gamma-1|inositoltrisphosphohydrolase|monophosphatidylinositol phosphodiesterase|phosphatidylinositol phospholipase C|phosphoinositidase C|phosphoinositide phospholipase C|phosphoinositide phospholipase C-gamma-1|phospholipase C, gamma 1 (formerly subtype 148)|phospholipase C-148|phospholipase C-II|phospholipase C-gamma-1|triphosphoinositide phosphodiesterase
Swissprots E1P5W4 Q2V575 B9EGH4 B7ZLY7 P19174
Accessions AAB70663 ABB84466 EAW75989 EAW75990 EAW75991 EAW75992 EAW75993 P19174 AB210028 BAE06110 AF147391 AK297370 BAG59815 AK309606 AL110247 CAB53696 BC136466 AAI36467 BC144136 AAI44137 BX415739 BX434193 M34667 AAA36452 XM_005260438 XP_005260495 XM_011528865 XP_011527167 XM_011528866 XP_011527168 XM_011528867 XP_011527169 XR_936550 NM_002660 NP_002651 NM_182811 NP_877963
Function Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand- mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, FGFR1, FGFR2, FGFR3 and FGFR4. Plays a role in actin reorganization and cell migration. {ECO:0000269|PubMed:17229814}.
Subcellular Location Cell projection, lamellipodium. Cell projection, ruffle. Note=Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment.
Top Pathways Epstein-Barr virus infection, MicroRNAs in cancer, Inflammatory mediator regulation of TRP channels, Choline metabolism in cancer, Rap1 signaling pathway

Phospho-PLCγ1 (Ser1248) (D25A9) Rabbit mAb (PE Conjugate) - 13349 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D25A9
Flow cytometric analysis of Jurkat cells treated with U0126 #9903 (blue) or TPA #4174 (green) using Phospho-PLCγ1 (Ser1248) (D25A9) Rabbit mAb (PE Conjugate).

Phospho-PLCγ1 (Tyr783) (D6M9S) Rabbit mAb (PE Conjugate) - 14461 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D6M9S
Flow cytometric analysis of NIH/3T3 cells, untreated (blue) or treated with recombinant mouse PDGF-BB (200 ng/ml, 10 min; green), using Phospho-PLCγ1 (Tyr783) (D6M9S) Rabbit mAb (PE Conjugate).

Phospho-PLCγ1 (Tyr783) Antibody - 2821 from Cell Signaling Technology

Prices $109.00, $287.00, $678.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from NIH/3T3 cells, untreated or PDGF-stimulated for the indicated times, using Phospho-PLCγ1 (Tyr783) Antibody (upper) or PLCγ1 Antibody #2822 (lower).

Phospho-PLCγ1 (Tyr783) (D6M9S) Rabbit mAb - 14008 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D6M9S
Western blot analysis of extracts from NIH/3T3 cells, untreated (-) or stimulated with hPDGF-BB #8912 (5 min; +), and from A-431 cells, untreated (-) or stimulated with hEGF #8916 (5 min; +), using Phospho-PLCγ1 (Tyr783) (D6M9S) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Phospho-PLCγ1 (Ser1248) (D25A9) Rabbit mAb - 8713 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D25A9
Western blot analysis of extracts from serum-starved A-431 and A549 cells, untreated (-) or treated (+) with hEGF #8916 (100 ng/mL, 15 min) or serum-starved NIH/3T3 cells, untreated (-) or treated (+) with hPDGF-BB #8912 (50 ng/mL, 15 min), using Phospho-PLCγ1 (Ser1248) (D25A9) Rabbit mAb (upper) or PLCγ1 (D9H10) XP ® Rabbit mAb #5690 (lower).

Phospho-PLCγ1 (Ser1248) Antibody - 4510 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Jurkat or COS cells, untreated or TPA/UV treated, using Phospho-PLCγ1 (Ser1248) Antibody.

PLCγ1 (D9H10) XP ® Rabbit mAb - 5690 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D9H10
Western blot analysis of extracts from various cell lines using PLCγ1 (D9H10) XP ® Rabbit mAb.

PLCγ1 Antibody - 2822 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from NIH/3T3 cells, untreated or PDGF-stimulated for the indicated times, using PLCγ1 Antibody.

Phospho-PLCgamma1 (Tyr771) Antibody - 2824 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from NIH/3T3 cells, untreated or PDGF-treated, using Phospho-PLCgamma1 (Tyr771) Antibody (upper) or PLCgamma1 Antibody #2822 (lower).

PLC γ1 (B-4) - sc-374467 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PLC γ1 (B-4): sc-374467. Western blot analysis of PLC γ1 expression in KNRK whole cell lysate.

PLC γ1 (1249) - sc-81 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PLC γ1 (1249): sc-81. Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic staining.

PLC γ1 (H-3) - sc-166938 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PLC γ1 (H-3): sc-166938. Western blot analysis of PLC γ1 expression in NIH/3T3 (A) and KNRK (B) whole cell lysates.

PLC γ1 (E-12) - sc-7290 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PLC γ1 (E-12): sc-7290. Western blot analysis of PLC γ1 expression in A-431 whole cell lysate.

PLC γ1 (N-20) - sc-31748 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PLC γ1 (N-20): sc-31748. Western blot analysis of PLC γ1 expression in A-431 (A), NIH/3T3 (B) and KNRK (C) whole cell lysates.

PLC γ1 (530) - sc-426 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PLC γ1 (530): sc-426. Western blot analysis of PLC γ1 expression in CHO-K1 (A), A-431 (B), NIH/3T3 (C) and KNRK (D) whole cell lysates.

PLC γ1 (1F1) - sc-58407 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
PLC γ1 (1F1): sc-58407. Western blot analysis of PLC γ1 expression in A-431 (A), NIH/3T3 (B), KNRK (C), MCF7 (D) and U-937 (E) whole cell lysates.

p-PLC γ1 (Tyr 1253) - sc-12944 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Western blot analysis of PLC γ1 phosphorylation in untreated (A,D), pervanadate treated (B,E) and pervanadate and lambda protein phosphatase treated (C,F) Jurkat whole cell lysates. Antibodies tested include p-PLC γ1 (Tyr 1253)-R: sc-12944-R (A,B,C) and PLC γ1 (E-12): sc-7290 (D,E,F).

p-PLC γ1 (Tyr 783) - sc-12943 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-PLC γ1 (Tyr 783): sc-12943-R. Immunoperoxidase staining of formalin fixed, paraffin-embedded human nasopharynx tissue showing cytoplasmic and nuclear staining of respiratory epithelial cells.

p-PLC γ1 (Tyr 771) - sc-293116 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
-PLC γ1 (Tyr 771): sc-293116. Western blot analysis of PLC γ1 phosphorylation expression in untreated (A) and serum-starved (B) 3T3 whole cell lysates.

p-PLC γ1 (pY783.27) - sc-136186 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
Western blot analysis of PLC γ1 phosphorylation in untreated (A,D), pervanadate treated (B,E) and pervanadate and lambda protein phosphatase treated (C,F) Jurkat whole cell lysates. Antibodies tested include p-PLC γ1 (pY783.27): sc-136186 (A,B,C) and PLC γ1 (E-12): sc-7290 (D,E,F).

Anti-Phospholipase C gamma 1 + 2 antibody - ab174787 from Abcam

Prices $407.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-Phospholipase C gamma 1 + 2 antibody (ab174787) + Jurkat cell lysate

Anti-Phospholipase C gamma 1 (phospho Y1253) antibody - ab73993 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Phospholipase C gamma 1 (phospho Y1253) antibody (ab73993) at 1/500 dilutionLane 1 : extracts from LOVO cellsLane 2 : extracts from 293 cellsLane 3 : extracts from LOVO cells with immunising phosphopeptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-Phospholipase C gamma 1 (phospho Y1253) antibody [EP1502Y] - ab81284 from Abcam

Prices $99.00, $313.00
Sizes 10 µl, 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1502Y
All lanes : Anti-Phospholipase C gamma 1 (phospho Y1253) antibody [EP1502Y] (ab81284) at 1/2000 dilutionLane 1 : Jurkat cell lysateLane 2 : Jurkat + Pervanadate cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP conjugated goat anti-rabbit at 1/2000 dilution

Anti-Phospholipase C gamma 1 (phospho Y771) antibody - ab131455 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence staining of methanol fixed HeLa cells labelling Phospholipase C gamma 1 (phospho Y771) with ab131455 antibody at a dilution of 1/100.

Anti-Phospholipase C gamma 1 (phospho Y771) antibody - ab52207 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
This image shows human breast carcinoma tissue stained with ab52207 at a dilution of 1/50 - 1/100. Right hand image: tissue treated with immunising peptide; left hand image: untreated.
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