EMD

Gene Symbol EMD
Entrez Gene 2010
Alt Symbol EDMD, LEMD5, STA
Species Human
Gene Type protein-coding
Description emerin
Other Description LEM domain containing 5
Swissprots Q6FI02 P50402
Accessions AAA92645 BAA10972 CAA60500 EAW72742 EAW72743 P50402 AM393723 CAL38599 BC000738 AAH00738 BT007401 AAP36065 CR536536 CAG38773 CX781694 X82434 CAA57817 NM_000117 NP_000108
Function Stabilizes and promotes the formation of a nuclear actin cortical network. Stimulates actin polymerization in vitro by binding and stabilizing the pointed end of growing filaments. Inhibits beta-catenin activity by preventing its accumulation in the nucleus. Acts by influencing the nuclear accumulation of beta- catenin through a CRM1-dependent export pathway. Links centrosomes to the nuclear envelope via a microtubule association. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD. Required for proper localization of non-farnesylated prelamin-A/C. {ECO:0000269|PubMed:15328537, ECO:0000269|PubMed:16680152, ECO:0000269|PubMed:16858403, ECO:0000269|PubMed:17785515, ECO:0000269|PubMed:19323649}.
Subcellular Location Nucleus inner membrane; Single-pass membrane protein; Nucleoplasmic side. Nucleus outer membrane. Note=Colocalized with BANF1 at the central region of the assembling nuclear rim, near spindle-attachment sites. The accumulation of different intermediates of prelamin-A/C (non- farnesylated or carboxymethylated farnesylated prelamin-A/C) in fibroblasts modify its localization in the nucleus.
Tissue Specificity Skeletal muscle, heart, colon, testis, ovary and pancreas.
Top Pathways Hypertrophic cardiomyopathy (HCM), Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy

Emerin (L12) Antibody - 2659 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell types using Emerin (L12) Antibody.

Emerin (D9B3) Rabbit mAb - 5430 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D9B3
Western blot analysis of extracts from HeLa, 293, and MCF7 cell lines using Emerin (D9B3) Rabbit mAb.

Emerin (A212) Antibody - 2660 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell types using Emerin (A212) Antibody.

emerin (8A1) - sc-81552 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
emerin (8A1): sc-81552. Western blot analysis of emerin expression in Saos-2 (A), K-562 (B), HeLa (C) and A549 (D) whole cell lysates.

emerin (F-11) - sc-398278 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
emerin (F-11): sc-398278. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear envelope localization.

emerin (G-10) - sc-398067 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
emerin (G-10): sc-398067. Western blot analysis of emerin expression in A549 whole cell lysate (A) and HeLa (B) and Jurkat (C) nuclear extracts.

emerin (H-7) - sc-393247 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
emerin (H-7): sc-393247. Western blot analysis of emerin expression in K-562 (A) and Jurkat (B) whole cell lysates and HeLa (C) and Jurkat (D) nuclear extracts.

emerin (FL-254) - sc-15378 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
emerin (FL-254): sc-15378.Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing nuclear envelope staining of exocrine pancreas and islet cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

emerin (C-20) - sc-8084 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
emerin (C-20): sc-8084. Western blot analysis of emerin expression in HeLa (A) and Saos-2 (B) whole cell lysates.

emerin (H-12) - sc-25284 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
emerin (H-12): sc-25284. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear envelope localization.

emerin (M-20) - sc-8086 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
emerin (M-20): sc-8086. Western blot analysis of emerin expression in A549 whole cell lysate.

emerin (N-19) - sc-8085 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
emerin (N-19): sc-8085. Western blot analysis of emerin expression in Saos-2 (A) and K-562 (B) whole cell lysates.

emerin (MANEM5) - sc-81553 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
emerin (MANEM5): sc-81553. Western blot analysis of emerin expression in Saos-2 (A), K-562 (B), HeLa (C) and A549 (D) whole cell lysates.

Anti-Emerin antibody - ab54996 from Abcam

Prices $385.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Overlay histogram showing HeLa cells stained with ab54996 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54996, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Emerin antibody - ab125494 from Abcam

Host Goat
Clonality Polyclonal
ab125494, at a 1/100 dilution, staining Emerin by Immunofluorescence.

Anti-Emerin antibody - ab153718 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded Human HeLa xenograft tissue, labeling Emerin with ab153718 at 1/1000 dilution.

Anti-Emerin antibody [4G5] - ab49499 from Abcam

Host Mouse
Clonality Monoclonal
Clone 4G5
Immunohistochemical staining (Paraffin embedded section) for Emerin using ab49499 with Human skeletal muscle as positive control. Note perinuclear staining of all cell nuclei.

Anti-Emerin antibody [4G5], prediluted - ab75446 from Abcam

Prices $376.00
Sizes 7 ml
Host Mouse
Clonality Monoclonal
Clone 4G5
Formalin-fixed, paraffin-embedded human skeletal muscle stained with ready to use ab75446 using peroxidase-conjugate and AEC chromogen. Note nuclear staining of muscle cells.

Anti-Emerin antibody [EPR11069] - ab169770 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR11069
All lanes : Anti-Emerin antibody [EPR11069] (ab169770) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : K562 cell lysateLysates/proteins at 10 µg/ml per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-Emerin antibody [EPR11071] - ab156871 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR11071
All lanes : Anti-Emerin antibody [EPR11071] (ab156871) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : Jurkat cell lysateLane 3 : A431 cell lysateLane 4 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Anti-Emerin antibody [MANEM1(5D10)] - ab125932 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone MANEM1(5D10)
Anti-Emerin antibody [MANEM1(5D10)] (ab125932) at 1/400 dilution + Normal Human fibroblast extracts

Anti-Emerin antibody - N-terminal - ab200589 from Abcam

Prices $359.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue labeling Emerin with ab200589 at 5 μg/ml.

Anti-Emerin antibody - N-terminal - ab157169 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-Emerin antibody - N-terminal (ab157169) at 1/1000 dilutionLane 1 : Mouse thymus total cell extractLane 2 : HeLa total cell extract

Anti-EMD / Emerin Antibody (aa157-173) - LS-C3198 from LifeSpan Bioscience

Prices $265.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-EMD / Emerin Antibody (N-Terminus) - LS-C23027 from LifeSpan Bioscience

Prices $325.00
Sizes 250 µl
Host Mouse
Clonality Monoclonal
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