MAP3K8

Gene Symbol MAP3K8
Entrez Gene 1326
Alt Symbol AURA2, COT, EST, ESTF, MEKK8, TPL2, Tpl-2, c-COT
Species Human
Gene Type protein-coding
Description mitogen-activated protein kinase kinase kinase 8
Other Description Ewing sarcoma transformant|augmented in rheumatoid arthritis 2|cot (cancer Osaka thyroid) oncogene|proto-oncogene c-Cot|proto-oncogene serine/threoine protein kinase|tumor progression locus 2
Swissprots D3DRX1 P41279 Q9HC81 Q14275 Q5T855 A8K2Q5
Accessions AAP45053 EAW86004 EAW86005 EAW86006 EAW86007 P41279 AA156603 AB209539 BAD92776 AK290320 BAF83009 AK313295 BAG36102 AL547407 BC022398 BC104833 AAI04834 BC113566 AAI13567 BR000407 FAA00321 CR542284 CAG47079 D14497 BAA03387 DA308072 DB271664 HQ258255 ADR83009 Z14138 CAA78512 XM_005252364 XP_005252421 XM_011519308 XP_011517610 XM_011519309 XP_011517611 XM_011519310 XP_011517612 XM_011519311 XP_011517613 XM_011519312 XP_011517614 XM_011519313 XP_011517615 XM_011519314 XP_011517616 XM_011519315 XP_011517617 NM_001244134 NP_001231063 NM_005204 NP_005195
Function Required for lipopolysaccharide (LPS)-induced, TLR4- mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the proinflammatory cytokine TNF- alpha (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. In vitro, activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner, leading to up-regulation of IL8 and CCL4. Transduces CD40 and TNFRSF1A signals that activate ERK in B- cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a ro
Subcellular Location Cytoplasm {ECO:0000269|PubMed:1833717, ECO:0000269|PubMed:8226782}.
Tissue Specificity Expressed in several normal tissues and human tumor-derived cell lines.
Top Pathways Toll-like receptor signaling pathway, T cell receptor signaling pathway, MAPK signaling pathway, TNF signaling pathway

Phospho-Tpl2 (Ser400) Antibody - 4491 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from COS cells, untransfected or transfected with rat Tpl2 , using Phospho-Tpl2 (Ser400) Antibody (upper) and Tpl2 Antibody #4492 (lower). In lane 3, Tpl2 transfected COS cell lysate was treated with λ phosphatase to demonstrate the phospho-specificity of Phospho-Tpl2 (Ser400) Antibody.

Tpl2 Antibody - 4492 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

Cot (H-7) - sc-373677 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cot (H-7): sc-373677. Western blot analysis of Cot expression in CTLL-2 whole cell lysate.

Cot (H-212) - sc-9167 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Cot (N-17) - sc-1717 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Cot (N-17): sc-1717. Western blot analysis of Cot expression in non-transfected: sc-117752 (A) and human Cot transfected: sc-372867 (B) 293T whole cell lysates.

Cot (M-20) - sc-720 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Cot (M-20): sc-720. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human tonsil tissue showing cytoplasmic and membrane staining.

Anti-MAP3K8 (phospho S400) antibody - ab111367 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab111367 at 1/50 dilution staining MAP3K8 in paraffin-embedded Human Brain tissue by Immunohistochemistry. The image on the right is treated with the synthesized phoshpopeptide.

Anti-MAP3K8 (phospho S400) antibody - ab192667 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling MAP3K8 (phospho S400) with ab192667 at 1/50 dilution in the absence (left) or presence (right) of blocking peptide.

Anti-MAP3K8 (phospho T290) antibody - ab51214 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded human brain tissue using MAP3K8 (phospho-Thr290) at a 1/50 dilution.Left image: Un-treatedRight image: Treated with phosphopeptide

Anti-MAP3K8 (phospho T290) antibody - ab195706 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MAP3K8 (phospho T290) antibody (ab195706) at 1/500 dilutionLane 1 : UV treated 293 whole cell lysate with antigen-specific peptideLane 2 : UV treated 293 whole cell lysate

Anti-MAP3K8 antibody - ab152104 from Abcam

Host Rabbit
Clonality Polyclonal
Anti-MAP3K8 antibody (ab152104) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

Anti-MAP3K8 antibody - ab51208 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded human brain tissue using ab51208, with and without immunising peptide.

Anti-MAP3K8 antibody - ab196751 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling MAP3K8 with ab196751 at a 1/50 dilution. The image on the right was treated with the synthesized peptide.

Anti-MAP3K8 antibody - ab94899 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-MAP3K8 antibody (ab94899) at 1 µg/ml + THP-1 cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-MAP3K8 antibody [EP1103Y] - ab52613 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1103Y
Anti-MAP3K8 antibody [EP1103Y] (ab52613) at 1/500 dilution + Jurkat cell lysate at 10 µgSecondaryGoat anti-rabbit HRP antibody at 1/2000 dilution

Anti-MAP3K8 / TPL2 Antibody (aa414-445) - LS-C99478 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Anti-MAP3K8 / TPL2 Antibody (C-Terminus, PE) - LS-C206496 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MAP3K8 / TPL2 Antibody (C-Terminus, FITC) - LS-C206495 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MAP3K8 / TPL2 Antibody (C-Terminus, AP) - LS-C206497 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MAP3K8 / TPL2 Antibody (C-Terminus, FITC) - LS-C206500 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MAP3K8 / TPL2 Antibody (C-Terminus, Biotin) - LS-C206498 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MAP3K8 / TPL2 Antibody (C-Terminus, PE) - LS-C206505 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MAP3K8 / TPL2 Antibody (C-Terminus, APC) - LS-C206499 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MAP3K8 / TPL2 Antibody (phospho-Thr290, Azide-free, HRP) - LS-C207956 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MAP3K8 / TPL2 Antibody (C-Terminus, Azide-free, HRP) - LS-C207958 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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