CDKN2B

Gene Symbol CDKN2B
Entrez Gene 1030
Alt Symbol CDK4I, INK4B, MTS2, P15, TP15, p15INK4b
Species Human
Gene Type protein-coding
Description cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4)
Other Description CDK inhibitory protein|CDK4B inhibitor|MTS-2|cyclin-dependent kinase 4 inhibitor B|cyclin-dependent kinases 4 and 6 binding protein|multiple tumor suppressor 2|p14-INK4b|p14_CDK inhibitor|p14_INK4B|p15 CDK inhibitor|p15-INK4b|p15_INK4B
Swissprots P42772 O15125 Q6FI09
Accessions AAB32712 AAD14049 AAD14186 AAF28397 AAM44859 CAH59951 EAW58596 EAW58597 P42772 AF004819 AAB69989 AF488409 AF488410 AAO49471 AF488411 AF488731 AM392641 CAL37519 AM392962 CAL37840 AM393264 CAL38142 AM393391 CAL38269 BC014469 AAH14469 BC018984 BI560960 CR536529 CAG38766 JQ694046 AFN61601 JQ694047 AFN61602 JQ694048 AFN61603 JX391994 AGC10270 L36844 AAA50282 U17075 AAC50075 NM_004936 NP_004927 NM_078487 NP_511042
Function Interacts strongly with CDK4 and CDK6. Potent inhibitor. Potential effector of TGF-beta induced cell cycle arrest.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:9230210}. Note=Also found in the nucleus.
Tissue Specificity Isoform 2 is expressed in normal (keratinocytes, fibroblasts) and tumor cell lines. {ECO:0000269|PubMed:9230210}.
Top Pathways Cell cycle, TGF-beta signaling pathway, Small cell lung cancer, HTLV-I infection, Viral carcinogenesis

p15 INK4B Antibody - 4822 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

p15 (D-12) - sc-271791 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p15 (D-12): sc-271791. Western blot analysis of p15 expression in non-transfected 293T: sc-117752 (A), human p15 transfected 293T: sc-129373 (B), A549 (C) and HeLa (D) whole cell lysates.

p15 (DCS114.1) - sc-65223 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

p15 (C-20) - sc-612 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
p15 (C-20): sc-612. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.

p15 (K-18) - sc-613 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

p15 (R-20) - sc-611 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Anti-p15 INK4b antibody - ab53034 from Abcam

Prices $390.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
p15 INK4b was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to p15 INK4b and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab53034.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 15kDa; p15 INK4b

Anti-p15 INK4b antibody - ab94688 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of p15 INK4b staining in human colon formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab94688, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-p15 INK4b antibody [DCS-114] - ab44891 from Abcam

Prices $378.00
Sizes 250 µl
Host Mouse
Clonality Monoclonal
Clone DCS-114

Anti-p15 INK4b antibody - C-terminal - ab170851 from Abcam

Prices $384.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-p15 INK4b antibody - C-terminal (ab170851) at 1/100 dilutionLane 1 : Non-transfected 293 cell line lysateLane 2 : p15 INK4-transfected 293 cell line lysateLysates/proteins at 2 µg per lane.

Anti-p15 INK4b antibody - N-terminal - ab170846 from Abcam

Prices $384.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Flow cytometric analysis of 293 cells, labeling p15 INK4b using ab170846 at a 1/10 dilution (right histogram), compared to negative control cells (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Anti-CDKN2B / p15 INK4b Antibody (aa109-138, PE) - LS-C213237 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CDKN2B / p15 INK4b Antibody (aa102-131, Biotin) - LS-C213238 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CDKN2B / p15 INK4b Antibody (aa109-138) - LS-C168626 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Confocal immunofluorescent of CDKN2B Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Anti-CDKN2B / p15 INK4b Antibody (Gly113) - LS-C176155 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of p15 INK (G113) pAb in paraffin-embedded human lung carcinoma tissue.

Anti-CDKN2B / p15 INK4b Antibody (aa102-131, PE) - LS-C213241 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CDKN2B / p15 INK4b Antibody (aa102-131, Azide-free, HRP) - LS-C213240 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CDKN2B / p15 INK4b Antibody (aa102-131, FITC) - LS-C213239 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CDKN2B / p15 INK4b Antibody (C-Terminus) - LS-C186568 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
CDKN2B antibody (1 ug/ml) staining of Human Gastrointestinal cancer lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CDKN2B / p15 INK4b Antibody (aa109-138, AP) - LS-C213242 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CDKN2B / p15 INK4b Antibody (aa109-138, APC) - LS-C213243 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CDKN2B / p15 INK4b Antibody (aa109-138, Biotin) - LS-C213244 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CDKN2B / p15 INK4b Antibody (aa109-138, Azide-free, HRP) - LS-C213246 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CDKN2B / p15 INK4b Antibody (aa109-138, PE) - LS-C213247 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CDKN2B / p15 INK4b Antibody (aa109-138, FITC) - LS-C213245 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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