ENPP2

Gene Symbol ENPP2
Entrez Gene 5168
Alt Symbol ATX, ATX-X, AUTOTAXIN, LysoPLD, NPP2, PD-IALPHA, PDNP2
Species Human
Gene Type protein-coding
Description ectonucleotide pyrophosphatase/phosphodiesterase 2
Other Description E-NPP 2|autotaxin-t|ectonucleotide pyrophosphatase/phosphodiesterase family member 2|extracellular lysophospholipase D|phosphodiesterase I/nucleotide pyrophosphatase 2|plasma lysophospholipase D
Swissprots Q15117 A8UHA1 Q13827 Q9UCR0 Q14555 Q13822 Q9UCR4 E9PHP7 Q9UCR1 Q9UCR3 Q9UCR2 Q9UCQ8
Accessions AAB00855 BAA08342 CCF77047 CCF77048 CCF77049 CCQ43347 EAW91986 EAW91987 EAW91988 EAW91989 EAW91990 Q13822 AA256687 AI339551 AI375195 AK124910 AK130313 AK296027 BAG58795 AK315253 AL544867 BC034961 AAH34961 BM694754 BQ365938 BU731264 CA390514 D45421 BAA08260 DA919550 DA922156 DB468753 DC338843 DQ891628 ABM82554 DQ894819 ABM85745 EU131011 ABW38316 L35594 AAA64785 XM_006716584 XP_006716647 XM_006716585 XP_006716648 XM_006716586 XP_006716649 XM_006716587 XP_006716650 NM_001040092 NP_001035181 NM_001130863 NP_001124335 NM_006209 NP_006200 NR_045555
Function Hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Also can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility- related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development. Tumor cell motility-stimulating factor. {ECO:0000269|PubMed:11559573, ECO:0000269|PubMed:1733949, ECO:0000269|PubMed:21240271}.
Subcellular Location Secreted {ECO:0000269|PubMed:15769751}. Note=Secreted by most body fluids including serum and CSF. Also by adipocytes and numerous cancer cells.
Tissue Specificity Predominantly expressed in brain, placenta, ovary, and small intestine. Expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. Expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. Not detected in leukocytes. Isoform 1 is more highly expressed in peripheral tissues than in the central nervous system (CNS). Adipocytes only express isoform 1. Isoform 3 is more highly expressed in the brain than in peripheral tissues. {ECO:0000269|PubMed:18175805, ECO:0000269|PubMed:8579579, ECO:0000269|PubMed:8586446}.
Top Pathways Ether lipid metabolism

Autotaxin (E-12) - sc-374222 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Autotaxin (E-12): sc-374222. Western blot analysis of Autotaxin expression in non-transfected: sc-117752 (A) and human Autotaxin transfected: sc-170647 (B) 293T whole cell lysates.

Autotaxin (H-180) - sc-66813 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Autotaxin (H-180): sc-66813. Western blot analysis of Autotaxin expression in non-transfected: sc-117752 (A) and mouse Autotaxin transfected: sc-118641 (B) 293T whole cell lysates.

Autotaxin (N-13) - sc-33075 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Autotaxin (N-13): sc-33075. Western blot analysis of Autotaxin expression in Hep G2 (A) and MOLT-4 (B) whole cell lysates.

Anti-ENPP2 antibody - ab133190 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of Formalin fixed, Paraffin-embedded Human brain cerebellum tissue labelling ENPP2 with ab133190 at 2.5 µg/ml, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-ENPP2 antibody - ab137590 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-ENPP2 antibody (ab137590) at 1/500 dilutionLane 1 : HepG2 whole cell lysateLane 2 : MOLT4 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-ENPP2 antibody - ab135527 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-ENPP2 antibody (ab135527) at 1/100 dilution + Mouse cerebellum tissue lysate at 35 µg

Anti-ENPP2 antibody - ab82383 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-ENPP2 antibody (ab82383) at 1/50 dilution + Y79 cell line lysates at 35 µg

Anti-ENPP2 antibody - ab181480 from Abcam

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-ENPP2 antibody (ab181480) at 1 µg/ml + Human placenta tissue lysate in RIPA buffer at 35 µgdeveloped using the ECL technique

Anti-ENPP2 antibody [1F8] - ab82590 from Abcam

Prices $378.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1F8
All lanes : Anti-HTR3C antibody (ab85290) at 1 µg/mlLane 1 : Ovary (Human) Tissue Lysate - adult normal tissue (ab30222)Lane 2 : Small Intestine (Human) Tissue Lysate - adult normal tissue (ab29276)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-ENPP2 antibody [1F8] (FITC) - ab150261 from Abcam

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 1F8

Anti-ENPP2 antibody [1F8] - BSA and Azide free - ab174725 from Abcam

Prices $372.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1F8

Anti-ENPP2 antibody [5H3] - ab77104 from Abcam

Prices $385.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 5H3
All lanes : Anti-ENPP2 antibody [5H3] (ab77104) at 10 µg/mlLane 1 : Placenta (Human) Tissue Lysate - adult normal tissue (ab29745)Lane 2 : Ovary (Human) Tissue Lysate - adult normal tissue (ab30222)Lane 3 : Small Intestine (Human) Tissue Lysate - adult normal tissue (ab29276)Lysates/proteins at 20 µg per lane.SecondaryMouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-ENPP2 antibody [5H3] (Alexa Fluor® 647) - ab196712 from Abcam

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5H3
ab196712 staining ENPP2 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196712 at a 1/50 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

Anti-ENPP2 antibody [5H3] - BSA and Azide free - ab175578 from Abcam

Prices $372.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 5H3

Anti-ENPP2 / Autotaxin Antibody (aa573-588) - LS-C37249 from LifeSpan Bioscience

Prices $465.00
Sizes 1 ea
Host Rabbit
Clonality Polyclonal

Anti-ENPP2 / Autotaxin Antibody (aa754-783, Biotin) - LS-C183850 from LifeSpan Bioscience

Prices $800.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ENPP2 / Autotaxin Antibody (aa754-783) - LS-C190758 from LifeSpan Bioscience

Prices $740.00
Sizes 200 µg
Host Rabbit
Clonality Polyclonal

Anti-ENPP2 / Autotaxin Antibody (aa369-391) - LS-C190757 from LifeSpan Bioscience

Prices $740.00
Sizes 200 µg
Host Rabbit
Clonality Polyclonal

Anti-ENPP2 / Autotaxin Antibody (aa754-783, FITC) - LS-C183851 from LifeSpan Bioscience

Prices $800.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ENPP2 / Autotaxin Antibody (aa754-783) - LS-C190759 from LifeSpan Bioscience

Prices $800.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ENPP2 / Autotaxin Antibody (aa377-406, AP) - LS-C277133 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ENPP2 / Autotaxin Antibody (aa377-406, Azide-free, HRP) - LS-C277135 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ENPP2 / Autotaxin Antibody (aa377-406, APC) - LS-C277134 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ENPP2 / Autotaxin Antibody (aa377-406, PE) - LS-C277136 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ENPP2 / Autotaxin Antibody (aa637-905, Biotin) - LS-C298179 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
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