CBL

Gene Symbol CBL
Entrez Gene 867
Alt Symbol C-CBL, CBL2, FRA11B, NSLL, RNF55
Species Human
Gene Type protein-coding
Description Cbl proto-oncogene, E3 ubiquitin protein ligase
Other Description Cas-Br-M (murine) ecotropic retroviral transforming sequence|E3 ubiquitin-protein ligase CBL|RING finger protein 55|casitas B-lineage lymphoma proto-oncogene|fragile site, folic acid type, rare, fra(11)(q23.3)|oncogene CBL2|proto-oncogene c-Cbl|signal transduction protein CBL
Swissprots P22681 A3KMP8
Accessions CAA57977 EAW67476 P22681 AA806454 AK092300 AL832396 BC132733 AAI32734 BC136463 AAI36464 BG722940 BX648139 DB457271 X57110 CAA40393 X69207 XM_011543057 XP_011541359 NM_005188 NP_005179
Function Adapter protein that functions as a negative regulator of many signaling pathways that are triggered by activation of cell surface receptors. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including KIT, FLT1, FGFR1, FGFR2, PDGFRA, PDGFRB, EGFR, CSF1R, EPHA8 and KDR and terminates signaling. Recognizes membrane-bound HCK, SRC and other kinases of the SRC family and mediates their ubiquitination and degradation. Participates in signal transduction in hematopoietic cells. Plays an important role in the regulation of osteoblast differentiation and apoptosis. Essential for osteoclastic bone resorption. The 'Tyr-731' phosphorylated form induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. May be
Subcellular Location Cytoplasm. Cell membrane. Note=Colocalizes with FGFR2 in lipid rafts at the cell membrane.
Top Pathways Proteoglycans in cancer, Ubiquitin mediated proteolysis, Bacterial invasion of epithelial cells, Endocytosis, ErbB signaling pathway

Phospho-c-Cbl (Tyr700) (D16D7) Rabbit mAb - 8869 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D16D7
Western blot analysis of extracts from Jurkat cells, untreated (-) or treated with pervanadate (1 mM; +), using Phospho-c-Cbl (Tyr700) (D16D7) Rabbit mAb (upper) or c-Cbl (D4E10) Rabbit mAb #8447 (lower).

Phospho-c-Cbl (Tyr731) Antibody - 3554 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from 293-T cells transfected with c-Cbl and Fyn (lanes 3 and 6) or without Fyn (lanes 2 and 5), untreated or calf intestinal phosphatase (CIP)-treated, using Phospho-c-Cbl (Tyr731) Antibody. (Lysates provided by Dr. A.L. Reddi, laboratory of Dr. Hamid Band, Harvard University, Massachusetts

Phospho-c-Cbl (Tyr774) Antibody - 3555 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from 293-T cells transfected with c-Cbl and Fyn (lanes 3 and 5) or without Fyn (lanes 2 and 4), untreated or calf intestinal phosphatase (CIP)-treated, using Phospho-c-Cbl (Tyr774) Antibody. (Lysates provided by Dr. A.L. Reddi, laboratory of Dr. Hamid Band, Harvard University, Massachusetts.)

c-Cbl Antibody - 2747 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of lysates from MCF-7, Raji, THP-1, RAW, COS and H-4-II-E cells, using c-CBL antibody.

c-Cbl (C49H8) Rabbit mAb - 2179 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C49H8
Western blot analysis of cell extracts from various cell types using c-Cbl (C49H8) Rabbit mAb.

c-Cbl (D4E10) Rabbit mAb - 8447 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D4E10
Western blot analysis of extracts from various cell lines using c-Cbl (D4E10) Rabbit mAb.

Cbl (A-9) - sc-1651 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cbl (A-9): sc-1651. Western blot analysis of Cbl p120 expression in K-562 whole cell lysate.

Cbl (C-3) - sc-393543 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cbl (C-3): sc-393543. Western blot analysis of Cbl expression in K-562 (A), HL-60 (B), A549 (C) and Hep G2 (D) whole cell lysates.

Cbl (G-12) - sc-398282 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cbl (G-12): sc-398282. Western blot analysis of Cbl expression in K-562 (A) and HL-60 (B) whole cell lysates.

Cbl (C-15) - sc-170 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Cbl (C-15): sc-170. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

Cbl (H-300) - sc-367372 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

p-Cbl (pY700.47) - sc-136013 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
p-Cbl (pY700.47): sc-136013. Western blot analysis of Cbl phosphorylation in untreated (A) and pervanadate-treated (B) Jurkat whole cell lysates.

p-Cbl (E-10) - sc-377571 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of Cbl phosphorylation in untreated (A,C) and lambda protein phosphatase (sc-200312A) treated (B,D) Jurkat whole cell lysates. Antibodies tested include p-Cbl (E-10): sc-377571 (A,B) and Cbl (A-9): sc-1651 (C,D).

Anti-CBL (phospho S669) antibody [EPR2226(2)] - ab108364 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2226(2)
All lanes : Anti-CBL (phospho S669) antibody [EPR2226(2)] (ab108364) at 1/1000 dilutionLane 1 : HeLa cell lysates, untreated Lane 2 : HeLa cell lysates, treated with pervanadateLysates/proteins at 10 µg per lane.

Anti-CBL (phospho Y674) antibody - ab58508 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-CBL (phospho Y674) antibody (ab58508) at 1/500 dilutionLane 1 : Extracts from HepG2 cells, treated with Na2VO3 (0.3nM, 40mins)Lane 2 : Extracts from HepG2 cells, treated with Na2VO3 (0.3nM, 40mins), plus immunising peptide

Anti-CBL (phospho Y674) antibody [EPR2227] - ab76536 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2227
All lanes : Anti-CBL (phospho Y674) antibody [EPR2227] (ab76536) at 1/1000 dilutionLane 1 : Jurkat cell lysateLane 2 : Jurkat cell lysate treated with pervanadateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/1000 dilution

Anti-CBL (phospho Y700) antibody - ab131345 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescent analysis of methanol-fixed HeLa cells labelling CBL with ab131345 at 1/100 dilution.

Anti-CBL (phospho Y700) antibody - ab202127 from Abcam

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescence analysis of methanol-fixed HeLa cells labeling CBL with ab202127 at 1/20 dilution. 

Anti-CBL (phospho Y700) antibody - ab63512 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab63512, used at a dilution of 1/100, staining HepG2 cells by immunofluoresence in the absence (1) and presence (2) of the phosphopeptide.

Anti-CBL (phospho Y700) antibody [EP2225Y] - ab76002 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2225Y
Flow cytometric analysis of ab76002 at 1/50 dilution staining permeabilized Jurkat cells, untreated (green) or pervanadate-treated (red) and pervanadate-treated Jurkat cells preincubated with immunising phospho-peptide (blue) or non-phospho peptide (orange).

Anti-CBL (phospho Y731) antibody [EP973Y] - ab52855 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP973Y
All lanes : Anti-CBL (phospho Y731) antibody [EP973Y] (ab52855) at 1/2000 dilutionLane 1 : Raji cell lysate untreatedLane 2 : Raji cell lysate treated with pervanadateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Anti-CBL (phospho Y774) antibody - ab58505 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-CBL (phospho Y774) antibody (ab58505) at 1/500 dilutionLane 1 : Extracts from HeLa cells, treated with EGF (200ng/ml, 30mins)Lane 2 : Extracts from HeLa cells, treated with EGF (200ng/ml, 30mins), plus immunising peptide

Anti-CBL (phospho Y774) antibody [E160] - ab32446 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone E160
All lanes : Anti-CBL (phospho Y774) antibody [E160] (ab32446) at 1/1000 dilutionLane 1 : Jurkat cell lysateLane 2 : Jurkat cell lysate + Pervanadate

Anti-CBL antibody - ab6077 from Abcam

Prices $378.00
Sizes 1 ml
Host Rabbit
Clonality Polyclonal
Ab6077 staining human normal colon tissue. Staining is localised to cytoplasm.Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-CBL antibody - ab93654 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-CBL antibody (ab93654) at 1/500 dilution + 293 cell lysate
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