CDK4

Gene Symbol CDK4
Entrez Gene 1019
Alt Symbol CMM3, PSK-J3
Species Human
Gene Type protein-coding
Description cyclin-dependent kinase 4
Other Description cell division protein kinase 4
Swissprots Q6FG61 P11802 B4DNF9 O00576 B2R9A0
Accessions AAC39521 AAC50506 AAD13991 AAM23014 CDI30203 CDM63717 EAW97058 EAW97059 EAW97060 EAW97061 EAW97062 P11802 AK297901 BAG60221 AK310724 AK313701 BAG36447 BC003644 AAH03644 BC005864 AAH05864 BC010153 AAH10153 BC015669 AAH15669 BI832658 BQ773564 CR407668 CAG28596 CR542247 CAG47043 DQ893137 ABM84063 DQ896420 ABM87419 M14505 AAA35673 U79269 AAB50213 Z48970 CAA88834 NM_000075 NP_000066 NM_052984
Function Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. {ECO:0000269|PubMed:15241418, ECO:0000269|PubMed:18827403, ECO:0000269|PubMed:9003781}.
Subcellular Location Cytoplasm. Nucleus. Membrane. Note=Cytoplasmic when non-complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus.
Top Pathways p53 signaling pathway, Tight junction, PI3K-Akt signaling pathway, T cell receptor signaling pathway, Viral carcinogenesis

Cdk4 CRISPR/Cas9 KO Plasmid (h) - sc-400148 from Santa Cruz Biotechnology

Category CRISPR
Prices $345.00
Sizes 20 µg
Species Reactivities Human

Cdk4 HDR Plasmid (h) - sc-400148-HDR from Santa Cruz Biotechnology

Prices $345.00
Sizes 20 µg
Species Reactivities Human

Cdk4 Double Nickase Plasmid (h) - sc-400148-NIC from Santa Cruz Biotechnology

Prices $345.00
Sizes 20 µg
Species Reactivities Human

Cdk4 Double Nickase Plasmid (h2) - sc-400148-NIC-2 from Santa Cruz Biotechnology

Prices $345.00
Sizes 20 µg
Species Reactivities Human

Cdk4 CRISPR Activation Plasmid (h) - sc-400148-ACT from Santa Cruz Biotechnology

Category CRISPR
Prices $345.00
Sizes 20 µg
Species Reactivities Human

Cdk4 CRISPR Activation Plasmid (h2) - sc-400148-ACT-2 from Santa Cruz Biotechnology

Category CRISPR
Prices $345.00
Sizes 20 µg
Species Reactivities Human

Cdk4 Lentiviral Activation Particles (h) - sc-400148-LAC from Santa Cruz Biotechnology

Prices $345.00
Sizes 20 µg
Species Reactivities Human

Cdk4 Lentiviral Activation Particles (h2) - sc-400148-LAC-2 from Santa Cruz Biotechnology

Prices $345.00
Sizes 20 µg
Species Reactivities Human

Cdk4 siRNA (h) - sc-29261 from Santa Cruz Biotechnology

Category siRNA
Prices $258.00
Sizes 10 µm
Species Reactivities Human

Cdk4 shRNA Plasmid (h) - sc-29261-SH from Santa Cruz Biotechnology

Category siRNA
Prices $258.00
Sizes 10 µm
Species Reactivities Human

Cdk4 shRNA (h) Lentiviral Particles - sc-29261-V from Santa Cruz Biotechnology

Category siRNA
Prices $258.00
Sizes 10 µm
Species Reactivities Human

CDK4 RNAi - H00001019-R01V from Novus Biologicals

Category siRNA
Prices $399.00
Sizes 20 nmol
Species Reactivities Human

CDK4 RNAi - H00001019-R02 from Novus Biologicals

Category siRNA
Prices $399.00
Sizes 20 nmol
Species Reactivities Human

MISSION® esiRNA - EHU070511 from Sigma-Aldrich

Category siRNA
Prices $140.00, $250.00
Sizes 20 µg, 50 µg
<B>Immunoblotting</B><BR/>CDK4: <B>Cat. No. EHU070511</B>: Western blot analysis of CDK4 in HeLa cells.<BR/><B>Lanes</B><BR/>1. HeLa cells transfected with Renilla Luciferase esiRNA (RLUC, <B>Cat. No. EHURLUC</B>) as negative control.<BR/>2. HeLa cells transfected with CDK4 esiRNA (<B>Cat. No. EHU070511</B>). Quantitative-Immunoblotting: Lysates were separated on SDS-Page and probed with mouse Monoclonal Anti-Cdk4 (1:1000) Clone: DCS-31 (<B>Cat. No. C8218</B>) and goat Anti-GAPDH (1:7000) (Acris) antibodies. The signal intensities of indicated bands were quantified using an Odyssey infrared imaging system (Li-COR) and normalized to the GAPDH signal. At 72h post transfection, a 70% CDK4 protein reduction was measured between the negative control RLUC and the sample treated with CDK4 esiRNA.

MISSION® siRNA - SIHK0351 from Sigma-Aldrich

Category siRNA
Prices $25.00
Sizes 0.25 nmol

MISSION® siRNA - SIHK0350 from Sigma-Aldrich

Category siRNA
Prices $25.00
Sizes 0.25 nmol

MISSION® siRNA - SIHK0352 from Sigma-Aldrich

Category siRNA
Prices $25.00
Sizes 0.25 nmol

CompoZr® Knockout ZFN Kit - CKOZFN5850 from Sigma-Aldrich

Category ZFN Kit
Prices $4,999.00
Sizes 1 kt

CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND5850 from Sigma-Aldrich

Category ZFN Kit
Prices $3,999.00
Sizes 1 kt

CDK4 siRNA (Human) - CRH0732 from Cohesion Biosciences

Category siRNA
Prices $340.00, $510.00
Sizes 15 nmol, 30 nmol
Species Reactivities Human

CDK4 - MBS8210582 from MyBioSource

Prices $365.00, $515.00
Sizes 15 nmol, 30 nmol
Species Reactivities Human

shRNA set against Human CDK4 (NM_000075.3) - SHH260613 from Creative biogene

Category siRNA
Species Reactivities Human

shRNA set against Human CDK4(NM_000075.3) - SHG164341 from Creative biogene

Category siRNA
Species Reactivities Human

Human CDK4 siRNA - orb261234 from Biorbyt

Category siRNA
Prices $485.00, $629.00
Sizes 15 nmol, 30 nmol
Species Reactivities Human

CDK4 shRNA Plasmid (Human) - CHH0732 from Cohesion Biosciences

Category siRNA
Prices $500.00, $750.00
Sizes 150 µg, 300 µg
Species Reactivities Human
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