NEDD8

Gene Symbol NEDD8
Entrez Gene 4738
Alt Symbol NEDD-8
Species Human
Gene Type protein-coding
Description neural precursor cell expressed, developmentally down-regulated 8
Other Description neddylin|ubiquitin-like protein Nedd8
Swissprots Q6LES6 Q3SXN8 Q15843
Accessions CDM22114 Q15843 AK125214 BC038290 BC064899 BC104200 AAI04201 BC104201 AAI04202 BC104664 AAI04665 BE548741 CF130271 CR407662 CAG28590 D23662 BAA04889 NM_006156 NP_006147
Function Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C- APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. {ECO:0000269|PubMed:10318914, ECO:0000269|PubMed:10597293, ECO:0000269|PubMed:11953428}.
Subcellular Location Nucleus {ECO:0000269|PubMed:9353319}. Note=Mainly nuclear.
Tissue Specificity Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes. {ECO:0000269|PubMed:10597293, ECO:0000269|PubMed:9353319}.

NEDD8 Antibody - 2745 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of Ubiquitin, NEDD8, ISG15 and SUMO-2/3 recombinant proteins (5 ng each), using NEDD8, Ubiquitin, ISG15 and SUMO-2/3 Antibodies.

NEDD8 (19E3) Rabbit mAb - 2754 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 19E3
Western blot analysis of ubiquitin, NEDD8, ISG15 and SUMO-2/3 recombinant proteins (5 ng each), using NEDD8 (19E3) Rabbit mAb, Ubiquitin (P4D1) Mouse mAb #3936, ISG15 Antibody #2743 and SUMO-2/3 Antibody #4974.

NEDD8 (H-2) - sc-373741 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NEDD8 (H-2): sc-373741. Western blot analysis of NEDD8 expression in BJAB whole cell lysate.

NEDD8 (FL-81) - sc-30077 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
NEDD8 (FL-81): sc-30077. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

NEDD8 (L-17) - sc-5478 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

NEDD8 (V-15) - sc-5480 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
NEDD8 (V-15): sc-5480. Western blot analysis of NEDD8 expression in BJAB (A) and K-562 (B) whole cell lysates.

NEDD8 (E-16) - sc-5479 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
NEDD8 (E-16): sc-5479. Western blot analysis of NEDD8 expression in Raji whole cell lysate.

Anti-NEDD8 antibody - ab38634 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-NEDD8 antibody (ab38634) at 1/100 dilution + HeLa cell lysates

Anti-NEDD8 antibody - ab131123 from Abcam

Prices $378.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human small intestine tissue, staining NEDD8 using ab131123 at a 1/100 dilution. This was followed by treatment with a biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-NEDD8 antibody - ab58399 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NEDD8 antibody (ab58399) at 1/500 dilutionLane 1 : Jurkat cell extractLane 2 : Jurkat cell extract with immunizing peptide

Anti-NEDD8 antibody - ab25988 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NEDD8 antibody [1A7] - ab124443 from Abcam

Host Mouse
Clonality Monoclonal
Clone 1A7
ab124443 at 1/200 dilution staining NEDD8 in Paraffin-embedded Human cervical cancer tissue by Immunohistochemistry followed by DAB staining.

Anti-NEDD8 antibody [Y297] - ab81264 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone Y297
All lanes : Anti-NEDD8 antibody [Y297] (ab81264) at 1/1000 dilutionLane 1 : C6 Cell LysateLane 2 : Raw264.7 Cell LysateLane 3 : PC-12 Cell LysateLane 4 : 3T3 Cell LysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-NEDD8 antibody [Y297] (Alexa Fluor® 647) - ab200849 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone Y297
ab200849 staining NEDD8 in A673 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab200849 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-NEDD8 Antibody (clone 5B8) IHC-plus™ - LS-B11079 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 5B8
Anti-NEDD8 antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11079 concentration 10 ug/ml.

Anti-NEDD8 Antibody - LS-C3368 from LifeSpan Bioscience

Prices $265.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-NEDD8 Antibody - LS-C9225 from LifeSpan Bioscience

Prices $515.00
Sizes 500 µg
Host Rabbit
Clonality Polyclonal

Anti-NEDD8 Antibody (N-Terminus) - LS-C9223 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-NEDD8 Antibody (aa20-32) - LS-C9226 from LifeSpan Bioscience

Prices $455.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NEDD8 Antibody (N-Terminus) - LS-C94349 from LifeSpan Bioscience

Prices $325.00
Sizes 500 µg
Host Sheep
Clonality Polyclonal

Anti-NEDD8 Antibody (aa2-77) - LS-C144032 from LifeSpan Bioscience

Prices $465.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-NEDD8 Antibody (N-Terminus, AP) - LS-C280019 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NEDD8 Antibody (N-Terminus, APC) - LS-C280018 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NEDD8 Antibody (aa20-32) IHC-plus™ - LS-B4950 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Anti-NEDD8 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4950 concentration 10 ug/ml.

Anti-NEDD8 Antibody (N-Terminus, Biotin) - LS-C280020 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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